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Objective:To establish a high performance liquid chromatography （HPLC） fingerprint of branches of <italic>Juglans mandshurica</italic> and to evaluate the quality of the samples from different producing areas and in different harvest periods. Method:Chromatographic separation was performed on an Agilent Poroshell 120 SB-C<sub>18</sub> column （2.1 mm×100 mm， 2.7 μm） for gradient elution with mobile phase of 0.2% formic acid solution （A）-0.2% formic acid acetonitrile solution （B） （0-5 min， 5%-10%B； 5-25 min， 10%-16%B； 25-40 min， 16%-22%B； 40-45 min， 22%-45%B； 45-50 min， 45%-65%B； 50-52 min， 65%-100%B； 52-55 min， 100%B） at a flow rate of 0.3 mL·min<sup>-1</sup>. The column temperature was 30 ℃ and the detection wavelength was 270 nm. The quality of branches of <italic>Juylans mandshurica</italic> was evaluated by similarity evaluation， cluster analysis， principal component analysis and partial least squares-discriminant analysis. The chemical constituents of the samples were identified by HPLC coupled with quadrupole time-of-flight mass spectrometry （HPLC-Q-TOF-MS/MS）. The mass spectrometry was conducted in negative ion mode with electrospray ionization（ESI）. Data were acquired over a range of <italic>m</italic>/<italic>z</italic> 100-1 700 for MS and <italic>m</italic>/<italic>z</italic> 50-1 700 for MS/MS. Result:A total of 19 common peaks were confirmed in 40 batches of samples， and the similarity ranged from 0.430 to 0.995， of which the similarity of samples collected in spring and winter seasons （April， May and December） was greater than 0.90， while the similarity of most samples collected in summer （July to September） was low. Multivariate statistical analysis showed that the samples were divided into two groups according to the harvest time， but there was no obvious classification rule for the samples from different producing areas. The contents of most constituents in the samples collected in spring and winter were higher than those collected in summer. The result illustrated that different harvest periods had great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>. Compared with the samples collected in summer， the quality of samples collected in spring and winter was better. A total of 22 peaks were proved to be the main constituents that contributed to the difference between samples collected in different seasons. A total of 83 chemical components were identified by HPLC-Q-TOF-MS/MS， including 49 tannins， 7 organic acids， 14 naphthalene derivatives， 1 flavonoid， 6 anthracene derivatives， 2 lignans， 3 diarylheptanoids and 1 saccharide. Totally 13 common peaks were identified. Of the peaks that contributed to discriminate samples collected in different season， 19 peaks were identified and most of them were tannins. Conclusion:The established HPLC fingerprint can provide useful information for the quality evaluation of branches of <italic>J</italic>.<italic> mandshurica</italic>. Tannin is the main constituents in the samples. Harvest period has great influence on the quality of branches of <italic>J</italic>.<italic> mandshurica</italic>.
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OBJECTIVE：To esta blish a method for si multaneous determination of 5 components in the branch and root of Juglans mandshurica as gallic acid ，ellagic acid ，1，6-di-O-galloyl-β-D-glucose，1，2，6-tri-O-galloyl-β-D-glucose and 1，2，3， 6-tetra-O-galloyl-β-D-glucose，and to analyze the content difference of above 5 components between the branch and root samples. METHODS：HPLC method was adopted. The determination was performed on Agilent Poroshell 120 SB-C18 column with mobile phase consisted of water （containing 0.2％ formic acid ）-acetonitrile （containing 0.2％ formic acid ）. A gradient elution was performed at a flow rate of 0.3 mL/min. The column temperature was 30 ℃ and the detection wavelength was 270 nm. The sample size was 5 μL. Independent samples t-test and partial least squares-discriminant analysis （PLS-DA）were applied for statistical analysis of 5 components. RESULTS ：The linear range of gallic acid ，ellagic acid ，1，6-di-O-galloyl-β-D-glucose，1，2， 6-tri-O-galloyl-β-D-glucose and 1，2，3，6-tetra-O-galloyl-β-D-glucose were 0.989-63.3，1.58-101，1.01-64.7，3.31-212，3.34-214 μg/mL （r≥0.997 3），respectively. RSDs of precision ，reproducibility and stability tests （12 h）were all lower than 3.2％. The average recoveries of the 5 components were 103.2％（RSD＝4.85％），99.1％（RSD＝2.80％），101.5％（RSD＝1.31％），102.9％（RSD＝ 2.73％）and 104.7％（RSD＝1.28％），respectively. The average contents of the above components in the branch of J. mandshurica were 0.296 5，0.621 1，0.562 5，3.111 7 and 3.451 3 mg/g，respectively. The average contents of above components in the root were 0.673 4，2.755 5，0.964 0，2.946 6 and 4.836 4 mg/g，respectively. The total contents of the 5 components in the branch and roo t of J. mandshurica were 8.043 2 and 12.175 9 mg/g，respectively. The contents of gallic acid ，ellagic acid and 1，6-di-O-galloyl- β-D-glucose in roots were significantly higher than those in branches （P＜0.05 or P＜0.01）. There were no significant differences in the contents of the other 2 components and the total contents of the 5 components in branches and roots （P＞0.05）. The cumulative interpretability （R 2X，R 2Y） and cumulative predictability （Q 2） of the model established by PLS-DA were 0.943，0.745，and 0.710 respectively. The model load diagram showed that the distance between the ellagic acid and the origin was the farthest ，and only variable projection importance of the content of the ellagic acid was greater than 1. CONCLUSIONS：The established method can be used for the content determination of 5 components in the branch and root of J. mandshurica . Except for 1，2，6-tri-O-galloyl-β-D-glucose，the contents of other 4 components and total contents of the 5 components in the root of J. mandshurica are higher than those of the branch. Ellagic acid is selected as the potential marker for discriminating the branch and root samples.
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Green walnut husks of Juglans mandshurica is the exocarp of the immature fruit of Juglans mandshurica. It has been used for thousands of years as a traditional Chinese herbal medicine. It is mainly used for antipyretic and detoxification, antibacterial and anti-inflammatory, analgesic and anti-malarial, and so on. In recent years, its anticancer activity has attracted widespread attention from domestic and foreign scientists. Modern pharmacological studies have shown that naphthoquinones are the main anticancer active ingredients of green walnut husks of J. mandshurica. In this review, we combed the naphthoquinone chemical components extracted from green walnut husks of J. mandshurica and described the chemical constituents of naphthoquinones with strong anticancer activity. It provides an important scientific basis for clarifying the pharmacodynamic basis of the anti-cancer effect of Chinese medicine green walnut husks of J. mandshurica.
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The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.
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Chromatography, High Pressure Liquid , Databases, Factual , Diarylheptanoids , Chemistry , Drugs, Chinese Herbal , Chemistry , Flavonoids , Chemistry , Fruit , Chemistry , Hydroxybenzoates , Chemistry , Juglans , Chemistry , Molecular Structure , Naphthoquinones , Chemistry , Reproducibility of Results , Tandem Mass Spectrometry , Triterpenes , ChemistryABSTRACT
Objective To analyze the chemical constituents from rat kidney tissue of Juglans mandshurica by UPLC-Q-TOF/MS. Methods The kidney tissue was collected after oral administration of ethanol extract of J. mandshurica. The gradient elution was performed using a Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), 0.1% formic acid water (A), and 0.1% formic acid acetonitrile (B) mobile phase system. An electrospray (ESI) ion source was used for mass spectrometry to collect data in positive ion mode. Combined with Peakview 2.0/masterview 1.0 and Metabolitepilot data analysis software, the kidney tissue components of J. mandshurica were identified by comparing the retention time, isotope kurtosis ratio, exact mass of the parent ion, and MS/MS fragment. Results Twenty-four chemical constituents including 16 prototypic components and eight metabolites were identified from rat kidney tissue, which contains 12 naphthoquinones, five flavonoids, three diarylheptanoids, and four triterpenoids. Conclusion The prototype components and metabolites of kidney tissue of J. mandshurica were determined. In further study, it provides reference for the safety and effectiveness of the treatment of J. mandshurica and provides a methodological reference for its in vivo composition and tissue distribution.
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Objective To study the chemical constituents of the n-butanol part which extracted from green walnut husks of Juglans mandshurica with antitumor activity. Methods The chemical constituents from 95% EtOH extract of green walnut husks of J. mandshurica at room temperature were isolated and prepared by silica gel column, reversed-phase ODS, and pre-HPLC after n-butanol extraction, and their structures were elucidated by a variety of spectral and spectroscopic techniques. Results Eight compounds were isolated and identified as 4-hydroxy-4-(3’-hydroxyphenol) butanoic acid-4-O-β-D-glucopyranoside ethyl ester (1), 4-hydroxy- 4-(3’-hydroxyphenyl) butyric acid-4-O-β-D-glucopyranoside methyl ester (2), 1,4,8-trihydroxynaphthalene-1-O-β-D-glucopyranoside (3), 1,4,8-trihydroxy-3-naphthoic acid ethyl ester-1-O-β-D-glucopyranoside (4), (4S)-4,5,8-trihydroxy-α-tetralone-4-O-β-D- glucopyranoside (5), (4S)-4,5,8-trihydroxy-α-tetralone-5-O-β-D-[6’-O-(3″,4″,5″-trihydroxybenzoyl)] glucopyranoside (6), (4S)-4- hydroxy-α-tetralone-4-O-β-D-(6’-O-4’’-hydroxylbenzoyl)-glucopyranoside (7), and (4S)-4,5-dihydroxy-α-tetralone-4-O-β-D-(6’-O- 4’’-hydroxylbenzoyl)-glucopyranoside (8). Conclusion Compound 1 named juglanoside P and compound 2 namded juglanoside Q are both new compounds.
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The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C column (2.1 mm × 100 mm, 1.7 μm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identifled by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.
Subject(s)
Chromatography, High Pressure Liquid , Databases, Factual , Diarylheptanoids , Chemistry , Drugs, Chinese Herbal , Chemistry , Flavonoids , Chemistry , Fruit , Chemistry , Hydroxybenzoates , Chemistry , Juglans , Chemistry , Molecular Structure , Naphthoquinones , Chemistry , Reproducibility of Results , Tandem Mass Spectrometry , Triterpenes , ChemistryABSTRACT
Objective To optimize extraction process of walnut quinone from Juglans green peel. Methods On the basis of single factor tests, taking material-liquid ratio, extracting temperature and ethanol concentration as independent variables, yield of walnut quinone in response to as a value, according to Box-Behnken experiment design principle, extraction process of walnut quinone from Juglans green peel was optimized by response surface analysis. Results The optimum extraction process of walnut quinone was: 14.30 times the amount of 89.81% ethanol;30.28 ℃ constant temperature. The extracting amount of walnut quinone was 2.034 mg/g, which was close to the experimental results of 1.957 mg/g. Conclusion The optimized extraction process is reasonable and feasible, which can provide reference for the extraction of walnut quinone from Juglans green peel.
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To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods. Twenty-six batches of exocarp of J. mandshurica samples from thirteen browning periods were assessed by UPLC-Q-TOF-MS/MS. The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 2.0/masterview1.0 software. Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references. The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred. Of the 13 naphthoquinone compounds, the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly, and 4 naphthoquinone derivatives such as regiolone were produced; the contents of four flavones and two phenolic acids compounds were decreased significantly; and the contents of 6 diarylheptanoids compounds were increased significantly. UPLC-Q-TOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately, and analyze the rules of dynamic changes, to reveal the browning of Chinese medicinal materials and its effects on compositions of fruits and vegetables.
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Objective: To study the chemical constituents from the exocarps of Juglans mandshurica which was an anti-tumor herb. Methods: The chemical constituents were isolated from the EtOAc and CHCl3 extracts by solvent extraction, repeated silica gel, Sephadex LH-20 column chromatography, and preparative high performance liquid chromatography. Their structures were elucidated by physicochemical properties and spectral data analysis. Results: Thirteen compounds were identified as 4(R)-ethoxy-8- hydroxy-α-tetralone (1), 4(R)-regiolone (2), ethyl gallate (3), Methyl 4-hydroxyphenylacetate (4), 8-hydroxyl anthraquinone-1- carboxylic acid (5), 4(R),5-dihydroxy-α-tetralone (6), myricanol (7), Juglanin B (8), 5-deoxymyricanone (9), 5-hydroxy-1- (4'-hydroxyphenyl)-7-(4″-hydroxy-3″-methoxyphenyl)-3-heptanone (10), broussonol E (11), Kaempferol-3-O-α-L-rhamnoside (12), quercetin-3-O-α-L-rhamnoside (13). Conclusion: Compounds 3-5, 7, and 9-13 are isolated from the exocarps of J. mandshurica for the first time, and the prenylated flavonoids were firstly reported in this genus.
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Objective: To identify the major chemical compounds in 78 batches of the exocarp of Juglans mandshurica from different origins by ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UFLC-Q-TOF/MS) and determine the major active chemical components. Methods: The separation was performed on Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), with a mobile phase using water with 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B) for gradient elution; Q-TOF/MS and electrospray ion (ESI) source were applied for the analysis under the positive ion mode; One thousand ions were extracted through Markerview 1.2.1 software from 78 batches. And common ions (compounds) were selected according to the following principles: One ion can be detected in all samples, and the relative strength is greater than the e4. Then the formula of common ions were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 2.0/masterview1.0 software. Their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references. Results: Thirty-one major chemical compounds including eleven naphthalene quinones, three diarylheptanoids, three flavonoids, eight triterpenes, and six other compounds were identified or inferred in the exocarp of J. mandshurica. Conclusion: UPLC-Q-TOF/MS method which develops a new strategy can identify the main chemical constituents from the exocarp of J. mandshurica rapidly and accurately, main chemical constituents can be used for the quality evaluation and efficacy material research.
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The changes in effective components of Juglans mandshurica at different harvest periods were analyzed by UPLC-Q-TOF-MS/MS. Eighteen batch samples of J. mandshurica from six harvest periods were assessed by multivariate statistical analysis with Markerview software. The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 2.0/Masterview1.0 software. Then their structure were determined by analysis of MS/MS fragment or comparison with standard substances and references. Naphthoquinone are the major markers in samples of Juglans mandshurica from different harvest periods. Thirty-eight of naphthalenequinones were identified or inferred in J. mandshurica and contents decline gradually. UPLC-Q-TOF-MS method which develops a new strategy can identify and analyze chemical constituents from J. mandshurica rapidly and accurately, main chemical constituents can be used for quality evaluation and efficacy material research. The dynamic changes in the metabolite accumulation of J. mandshurica the basic data for harvesting medicinal plants at different times.
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Two new naphthalenone compounds were isolated from green walnut husks of Juglans mandshurica and their structures were identified as 4-butoxybutoxy-5,8-dihydroxy-3,4-dihydro-2H-naphthalen-1-one (1), 4-ethoxyethoxy-5,8-dihydroxy-3,4-dihydro-2H-naphthalen-1-one (2). Compounds 1 and 2 were named as Juglanstetralone A (1) and Juglanstetralone B (2). Compound 1 showed more significant anti-tumor activity than 2 against gastric cancer BGC-823 cells, wih the IC50 of 125.89 (g(mL(-1).
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Humans , Antineoplastic Agents, Phytogenic , Pharmacology , Therapeutic Uses , Carcinoma , Drug Therapy , Cell Line, Tumor , Juglans , Chemistry , Naphthols , Pharmacology , Therapeutic Uses , Nuts , Chemistry , Phytotherapy , Plant Extracts , Chemistry , Pharmacology , Therapeutic Uses , Stomach Neoplasms , Drug TherapyABSTRACT
Objective: To study the chemical constituents in n-butanol extracts from the epicarp of green fruits of Juglans mandshurica. Methods: The compounds were isolated and purified by silica gel, ODS, Sephadex LH-20 columns, high preparative performance liquid chromatography, etc. And their structures were identified by spectral analysis. Results: Fourteen compounds were obtained and identified as 5-O-cafffeoyl quinic acid butyl ester (1), 3,5-di-O-caffeoylquinic acid butyl ester (2), vanillic acid-4-O-β-D-(6'-O-galloyl) glucopyranoside (3), 4-hydroxy-2,6-dimethoxyphenol-1-O-β-D-glucopyranoside (4), 4,5,8-trihydroxy-α-tetralone-5-O-β-D-glucopyranoside (5), 1,4,8- trihydroxynaphthalene-1-O-β-D-glucopyranoside (6), 1,4,5-trihydroxynaphthalene-1,4-di-O-β-D-glucopyranoside (7), kaempferol-3-O-β- D-glucopyranoside (8), quercetin-3-O-β-D-glucopyranoside (9), quercitrin (10), myricitrin (11), afzelin (12), hyperin (13), and daucosterol (14). Conclusion: Compounds 1-4 are isolated from the plants of Juglans L. for the first time, Compounds 5, 8-12 are isolated from the epicarps of J. mandshurica for the first time.
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The present study was designed to isolate and characterize novel chemical constituents of the stem bark of Juglans mandshurica Maxim. (Juglandaceae). The chemical constituents were isolated and purified by various chromatographic techniques. The structures of the compounds were elucidated on the basis of spectral data (1D and 2D NMR, HR-ESI-MS, CD, UV, and IR) and by the comparisons of spectroscopic data with the reported values in the literatures. Two long chain polyunsaturated fatty acids (1 and 2) were obtained and identified as (S)-(8E,10E)-12-hydroxy-7-oxo-8,10-octadecadienoic acid (1) and (S)-(8E, 10E)-12-hydroxy-7-oxo-8,10-octadecadienoic acid methyl ester (2). To the best of our knowledge, this is the first report on the isolation and structural elucidation of the two new conjugated ketonic fatty acids from this genus.
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Fatty Acids, Unsaturated , Chemistry , Juglans , Chemistry , Plant Bark , Chemistry , Spectrum AnalysisABSTRACT
Objective: To study the chemical constituents from the effective fraction in pericarps of Juglans mandshurica. Methods: The compounds were isolated and purified by various chromatographic methods, including silica gel, Sephadex LH-20, and preparative high performance liquid chromatography. Their structures were elucidated by NMR and MS analyses. Results: Fifteen compounds were obtained and identified as kaempferol (1), oleanolic acid (2), chrysophanol (3), pinostrobin (4), (2S)-onysilin (5), juglanin B (6), (S)-regiolone (7), chlorogenic acid (8), (4S)-4-hydroxy-1-tetralone (9), (4S, 5S, 7R, 8R, 14R)-8, 11-dihydroxy-2, 4-cyclo-eudesmane (10), 5-hydroxy-3, 7, 3', 4'-tetramethoxyflavone (11), (2S)-5, 7, 4'-trihydroxy-dihydroflavonol (12), p-hydroxybenzonic acid (13), p-methoxyphenylacetic acid (14), and 1, 4-dihydroxybenzene (15). Conclusion: Compounds 8-15 are first isolated from the pericarps of J. mandshurica and compounds 11, 12, 14, and 15 are obtained from the plants of Juglans L. for the first time.
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Objective To evaluate the effects of alcohol extract of juglans mandshurica maxim (AEBJ) on immune function of ionizing irradiated mice.Methods The mice were randomly divided into normal control group,irradiating control group,low AEBJ(300 mg?kg-1)irradiating group,high AEBJ(600 mg? kg-1)irradiating group,low AEBJ plus drugs only group and high AEBJ plus drugs only group.The number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation were examined.Results Compared with normal control group,the number of WBC and LYMPH,LYMPH%,viscera index,ability of lymphocytes transformation in irradiating control group were decreased significantly (P
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Objective To study the chemical constituents from the bark of Juglans mandshurica. Methods The compounds were isolated and purified by column chromatography on silica gel,HPLC,and recrystallization.Their structures were elucidated by the physicochemical and spectroscopic evidences. Results Fifteen compounds were identified as:4,8-dihydroxynaphthalenyl-O-?D-(6′-acetoxyl)gluco- pyranoside(Ⅰ),dihydrokaempferol(Ⅱ),juglone(Ⅲ),daucosterol(Ⅳ),kaempferol(Ⅴ),4,8-dihy- droxynaphthalenyl-1-O-?-D-[6′-O-(3″,5″-dimethoxy-4″-hydroxybenzoyl)] glucopyranoside(Ⅵ), kaempferol-3-O-?-L-rhamnoside(Ⅶ),3,3′-dimethoxylellagic acid(Ⅷ),naringenin(Ⅸ),quercetin (Ⅹ),reginolone(Ⅺ),quercetin-3-O-?-L-rhamnoside(Ⅻ),naringenin-7-O-?-D-glucoside(ⅩⅢ),4,8- dihydroxynaphthalenyl-1-O-?-glucoside(ⅩⅣ),4,5,8-trihydroxy-?-tetralone-5-O-?-D-[6′-O-(4″-hy- droxy-3″,5″-dimethoxy-benzoyl)] glucoside(ⅩⅤ).Conclusion CompoundⅠ(juglamanol)is a new compound.CompoundsⅡ,Ⅶ—Ⅸ,Ⅻ,andⅩⅢare isolated from plants of Carya Nutt.for the first time.
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Objective To study the chemical components from the pericarps of Juglans mandshurica with anti-tumor activity.Methods It showed that pericarps of J.mandshurica had a good performance in treating cancer and low toxicity from CHCl3 and EtOAc portions by a lot of pharmacological experiments and modern clinical application in the past.Compounds were isolated by chromatography on silica gel columns and AB-8 macroporous resin.On the basis of spectral evidences including 1D-NMR and 2D-NMR,the structures of these compounds were determined.Results These compounds from CHCl3 portion were identified as 20(S)-protopanaxadiol-3-one(1),dammara-20,24-dien-3?-ol(2),galeon(3),juglanin A(4),2?,3?,23-trihydroxy olean-12-en-28-oic acid(5),2?,3?,23-trihydroxy urs-12-en-28-oic acid(6),oleanolic acid(7),ursolic acid(8).Conclusion Compound 1 is a new natural product.Compounds 2 and 5-8 are isolated firstly from the plants of Juglans L.which are due to pentacyclic triterpane.Compound 3 is isolated from the pericarps of J.mandshurica for the first time.
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Objective To study the chemical constituents from the exocarp of Juglans mandshurica.Methods The compounds were isolated by Diaion HP-20,Sephadex LH-20,MCI CHP-20,and silica gel column chromatography.Their structures were identified by physicochemical properties and spectroscopic analysis.Results Twelve compounds were elucidated as pinostrobin (Ⅰ),quercetin (Ⅱ),quercetin-3-O-?-D-glucoside (Ⅲ),chrysophanol (Ⅳ),gallic acid (Ⅴ),4-hydroxyl-cinnamic acid methyl ester (Ⅵ),vanillin (Ⅶ),caffeic acid (Ⅷ),4-hydroxylcinnamic acid (Ⅸ),?-sitosterol (Ⅹ),daucosterol (Ⅺ),and 5-hydroxyl-1,4-naphthoquinone (ⅩⅡ).Conclusion The compounds Ⅰ,Ⅳ,and Ⅵ—Ⅸ are isolated from J.mandshurica for the first time.