Chemical constituents from stems and leaves of Artabotrys hongkongensis / 中草药

Objective: To study the chemical constituents from the stems and leaves of Artabotrys hongkongensis. Methods: The chemical constituents of A. hongkongensis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatography, and preparative HPLC. Their chemical structures were identified by physicochemical properties and spectral data. Results: A total of 16 compounds were isolated from the 90% ethanol extract of the stems and leaves of A. hongkongensis, which were identified as melilotocarpan A (1), medicarpin (2), 5,7,8-trimethoxyflarone (3), 2’-hydroxy-3’,4’,6’-trimethoxychalcone (4), piperolactam C (5), uvarilactam (6), artacinatine (7), p-coumaroyl-β-phenethylamine (8), artapilosol A (9), uvarigranol B (10), 1,6-desoxypipoxide (11), 1β,11-dihydroxy-5-eudesmen (12), loliolide (13), 4-hydroxy-3-methoxybenzaldehy (14), syringlaldehyde (15), and rabdosia acid A (16). Conclusion: All compounds are isolated from A. hongkongensis for the first time. In addition to compounds 2, 7, and 9, the other compounds are isolated from the genus Artabotrys for the first time.

Chemical constituents from stems and leaves of Mappianthus iodoides / 中草药

Objective To study the chemical constituents of the stems and leaves of Mappianthus iodoides. Methods The chemical constituents of M. iodoides were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Sixteen compounds were isolated from the 90% ethanol extract of the stems and leaves of M. iodoides, which were identified as 9-hydroxy-4,6-megastigmadien-3-one (1), 9-hydroxy-4,7-megastigmadien-3-one (2), blumenol A (3), 9,10-dihydroxy-4,7-megastigmadien-3-one (4), 5,12-epoxy-9-hydroxy-7-megastigmen-3-one (5), 5,12-epoxy-6,9-hydroxy-7- megastigmen-3-one (6), loliolide (7), lariciresinol (8), isolariciresinol (9), 5′-methoxylariciresinol (10), olivil (11), dehydrodiconiferyl alcohol (12), chushizisin I (13), 3,3-didemethoxyverrucosin (14), 4-epi-larreatricin (15), and glycerol monolinoleate (16). Conclusion All compounds are isolated from the genus Mappianthus for the first time.

Chemical constituents from leaves of Magnolia delavayi / 中草药

Objective To study the chemical constituents from the leaves of Magnolia delavayi. Methods The chemical constituents were isolated and purified by column chromatography on silica gel, Sephadex LH-20, MCI, and HPLC. Their structures were identified based on spectroscopic data. Results Fourteen compounds were isolated from 95% ethanol aqueous extract of M. delavayi and the structures were identified as (2E)-3,7,11-trimethyl-2,10-dodecadien-l,6,7-triol (1), (2E,6E)-3,7,11-trimethyl-2,6-dodecadien-l,10,11- triol (2), loliolide (3), pinoresinol (4), syringaresinol (5), medioresinol (6), eudesmin (7), phillygenin (8), coniferaldehyde (9), 3,4,5-trimethoxycinnamyl alcohol (10), indole-3-carboxaldehyde (11), indole-3-ethanol (12), 3,4-dimethoxy-benzoic acid (13), and 3,4-dimethoxyphenol (14). Conclusion All compounds are isolated from this plant for the first time. In addition, the 1H- and 13C-NMR spectroscopic data are reported for the first time in the current study.

Chemical constituents of Glechoma longituba / 中草药

Objective To study the chemical constituents of Glechoma longituba. Methods Chemical constituents were extracted by organic solvents, isolated and purified by chromatographic techniques with silica gel, Sephadex LH-20, semi-preparative HPLC, and recrystallization. Their structures were elucidated on the basis of physiochemical and spectral analyses. Results Thirteen compounds were obtained and elucidated as ursolic acid (1), asoleanolic acid (2), protocatechualdehyde (3), betulic acid (4), luteolin (5), β-sitosterol (6), lutin (7), syringic acid (8), 4-acetoxy-3,5-dimethoxybenzoicacid (9), 2,5-dihydroxy-1,4-benzenedicarboxylic acid (10), (E)-3-[4-(carboxymethoxy)-3-methoxyphenyl] acrylic acid (11), emodin (12), and loliolide (13). Conclusion Compounds 8-10 and 12 are isolated from this plant for the first time. Compounds 11 and 13 are isolated from the plants of Glechoma Linn. for the first time.

Chemical constituents of whole plants of Patrinia villosa (Thunb.) Juss / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Whole Plants of Patrinia villosa (Thunb.) Juss. METHODS: Compounds were isolated by a combination of various chromatographic techniques including column chromatography over macroporous resin, Sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by physiochemical properties and spectral analysis. RESULTS: Nine compounds were isolated and identified as (E, 4R)-4-hydroxy-4, 5, 5-trimethyl-3-(3-oxobut-1-enyl)cyclohex-2-enone (1), vomifoliol (2), cis-4-hydroxymellein (3), dehydrovomifoliol (4), 3R, 5S, 6R, 7E, 9S-3, 5, 6, 9-tetrahydroxy-7-megastigmane (5), loliolide (6), 4-methoxybenzyl alcohol (7), sphenanthin A (8), and urea (9). CONCLUSION: Compounds 1-7 are isolated fromPatrinia genus for the first time.

Terpenoids from Euphorbia micractina / 药学学报

From an ethanol extract of Euphorbia micractina roots, sixteen terpenoids were isolated by a combination of various chromatographic techniques, including column chromatography over macroporous resin, silica gel, and Sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by spectroscopic data analysis as loliolide myristate (1), 24-methylenetirucall-8-en-3β,11α-diol-7-one (2), loliolide (3), 3β-hydroxy-5α,6α-epoxy-7-megastigmen-9-one (4), jolkinol A (5), jolkinol D (6), latilagascene F (7), helioscopinolide A (8), helioscopinolide B (9), 3-O-acetylhelioscopinolide B (10), helioscopinolide D (11), helioscopinolide E (12), (+)-11-acetoxyatis-16-en-3,14-dione (13), erythrodiol (14), uvaol (15) and betulin (16). All of the compounds were obtained from this plant for the first time, in which 1 and 2 are new compounds.

Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (-)-Loliolide by HPLC

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β-sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, ¹H-, and ¹³C-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.

Chemical constituents from the stem bark of pandanus tectorius / 中国药学杂志

OBJECTIVE: To study the chemical constituents in the stem bark of Pandanus tectorius Soland. METHODS: Compounds were isolated by repeated chromatography. Their structures were elucidated by spectroscopic methods. RESULTS: Thirteen compounds were isolated and identified as 3-hydroxy-2-isopropenyldihydrobenzofuran-5-carboxylic acid methyl ester (1), pinoresinol (2), epi-pinoresinol(3), salicifoliol(4), thero-2, 3-bis-(4-hydroxy-3-methoxyphenyl) -3-ethoxypropan-1-ol(5), erythero-2, 3-bis-(4-hydroxy-3-methoxyphenyl) -3-ethoxypropan-1-ol(6), eudesmin A (7), loliolide (8), pinoresinol-4-O-β-D-glucoside (9), protocate-chuic acid(10), caffeic acid(11), p-hydroxybenzoic acid (12), and ferulic acid (13). CONCLUSION: All the compounds are isolated from this plant for the first time.

Sesquiterpenoids from branches and leaves of Cephalotaxus lanceolata / 中草药

Objective: To isolate and identify the chemical constituents from the branches and leaves of Cephalotaxus lanceolata. Methods: The compounds were separated and purified by column chromatography with silica gel, Sephadex LH-20, MPLC, and HPLC. Their structures were elucidated on the basis of spectroscopic methods. Results: Eleven sesquiterpenoids were isolated from 95% ethanol extract of the branches and leaves of C. lanceolata and identified as lanceoloside A (1), lanceoloside B (2), lanceoloside C (3), 9-hydroxy-4,7-megastigmadien-3-one (4), corchoionol C (5), 9,10-dihydroxy-4,7-megastigmadien-3-one (6), 5,12-epoxy-9- hydroxy-7-megastigmen-3-one (7), 5,12-epoxy-6,9-hydroxy-7-megastigmen-3-one (8), loliolide (9), (3S,5R,8S)-5,8-epoxy-6- megastigmadien-3,9-diol (10), and clovandiol (11) respectively. Conclusion: Compounds 1 is identified as a new sesquiterpenoid, while compounds 2-3 are two new natural products. Compounds 4-11 are isolated from this plant for the first time.

Chemical constituents from Pothos chinensis / 中草药

Objective: To investigate the chemical constituents in the whole herbs of Pothos chinensis. Methods: The chemical constituents were isolated and purified by various column chromatographic methods and preparative HPLC. Their structures were identified by physicochemical properties and spectral analyses. Results: Seventeen compounds were isolated from the methanol extract and identified as N-trans-cinnamoyltyramine (1), N-trans-p-coumaroyltyramine (2), N-cis-p-coumaroyltyramine (3), N-trans- feruloyltyramine (4), N-cis-feruloyltyramine (5), loliolide (6), dehydrovomifoliol (7), (3S, 5R, 8R, 9R)-5, 8-epoxy-6-megastigmene-3, 9-diol (8), (3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one (9), (3R)-4-[(2R, 4S)-2-hydroxy-2, 6, 6-trimethylcyclohexylidene]-3- buten-2-oneiol (10), 7-methoxycoumarine (11), imperatorin (12), 7-hydroxy-8-methoxycoumarin (13), 6-hydroxy-7-methoxycoumarin (14), indole-3-aldehyde (15), (-)-medioresinol (16), and daidzein (17). Conclusion: All the compounds are isolated from the plants in Pothos L. for the first time.

Chemical constituents in leaves of Ostryopsis nobilis and their anti-oxidant activities / 中草药

Objective: To study the chemical constituents of Ostryopsis nobilis and their anti-oxidant activities. Methods: The chemical constituents were isolated and purified by various column chromatographic methods. Their structures were identified by physicochemical properties and spectral analyses. Results: Twenty-three compounds were isolated from the 95% ethanol extract and identified as loliolide (1), maslinic acid (2), vanillic acid (3), 3β-(3, 4-dihydroxycinnamoyl)-erythrodiol (4), dammarenediol II 3-caffeate (5), pinoresinol (6), quercetin (7), daucosterol (8), kaempferol (9), 3, 5-dihydroxy-1, 7-bis (4H-hydroxyphenyl) heptane (10), alnusdiol (11), casuarinondiol (12), quercetin-3-O-α-L-arabinoside (13), isoquercetin (14), 2, 3-dihydroxylbenzoic acid (15), isoquercetin-6″-butyl acetate (16), isoquercetin-6″-benzoate (17), 4″-trans-p-coumaroyl-kaempferol-3-O-α-L-rhamnoside (18), 4″-cis-p-coumaroyl-kaempferol-3-O-α-L-rhamnoside (19), maslinic acid-28-O-β-D-glucoside (20), gallic acid (21), betulatetraol (22), and L-chiroinositol (23). Conclusion: All the compounds are isolated from the plants in this genus for the first time. Diarylheptanoid compounds and other ten monomer compounds exhibit the good scavenging activities against 2, 2-diphenyl-1-picryhydrazyl (DPPH), and O. nobilis extracts show moderate anti-oxidant effects.

Chemical constituents from marine alga Sargassum thunbergii / 中国药学杂志

OBJECTIVE: To study the chemical constituents of Sargassum thunbergii. METHODS: The compounds were isolated and purified upon extensive column chromatography using silica gel, ODS, Sephadex LH-20, and prepative HPLC, while their structures were identified on the basis of physicochemical properties and spectroscopic data analysis. RESULTS: Fifteen compounds were isolated from the EtOH extract. The structures were identified as(+)-isololiolide(1), (-)-loliolide(2), loliolide acetate(3) grasshopper ketone(4), apo-9β-fucoxanthinone(5), 3α-hydroxy-5, 6-epoxy-7-megastigmen-9-one(6), deacetylate-apo-13'-fucoxanthinone(7), apo-13'-fucoxanthinone(8),(R)-(-)-3-hydroxy-β-ionone(9) and 6 other compounds sargassum ketone(10), 2-heptenoicacid, 4,4-dihydroxy-2,3-dimethyl-7-lactone(11), (R)-2-hydroxy-3-phenylpropanamide(12), thymidine(13), fucosterol(14) and hexade-canoic acid-2β, 3β-dihydroxypropyl ester(15). CONCLUSION: Deacetylate-apo-13'-fucoxanthinone is a new compound, and compounds 3,5,6,8,9,15 are obtained from the genus Sargassum for the first time.