ABSTRACT
OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophather-um gracile. METHODS:HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile- water (gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 280 nm,column temperature was 35 ℃,and the injec-tion volume was 10 μl. RESULTS:The linear range was 0.041 0-1.228 8 μg for chlorogenic acid(r=0.999 8)and 0.264 0-7.920 0μg for vitexin(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.6%-102.3%(RSD=1.85%,n=9) and 97.1%-101.3%(RSD=1.19%,n=9),respectively. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.
ABSTRACT
Objective To study the optimum ethanol extraction process of total flavonoids in Lophatherun gracile Brongn. Methods The extraction of total flavonoids was choosen as the assessment index. Ethanol volumn extracting time and times were the studing factors. The optimum ethanol extraction process was selected with the orthogonal design. Results The optimum ethanol extracting process conditions were as follows:adding 18 fold of 75% ethanol,extracting for 1.0 h and one in all. Ethanol dosage was the predominant influencing factor. Conclusion This optimum extradion process of total flavonoids in Lophatherum gracle Brongn is reliable.