ABSTRACT
Recently, studies on Lycoris Herb. type alkaloids received the attention of scholars home and abroad. Lycoris Herb. type alkaloids can be devided into seven types according to their molecular structure, including lycorine type, crinine type, galanthamine type, tazettine type, narciclasine type, lycorenine type, homolycorine type, and montanine type. Researches have shown that Lycoris Herb. type alkaloids possess multiple phamocology activities, such as strong antitumor activity on human breast cancer cell (MCF-7), human leukemia cell (HL-60); and strong inhibitory effect of flu virus, measles virus, polio virus, and SARS virus; Lycorine type and crinine type alkaloids have antimalaria effect; Besides, lycorine type has strong anti-acetylcholinesterase effect. In a word, lycorine type and lycoris type alkaloids carry multiple pharmacological effect and belong to promising substances.
ABSTRACT
Objective: To investigate the interspecific relationships among thirteen species (include two varieties) of Lycoris Herb, from different habitats and provide the evidence for evaluation and exploitation of the germplasm resources for Lycoris Herb. Methods: The genetic diversities of 20 accessions of Lycoris Herb, from different habitats were investigated with the technique of amplified fragment length polymorphism (AFLP). Results: Six pairs of AFLP primers were selected from 33 pairs of combinations, and a total of 330 amplified clear DNA bands were obtained, among them 320 were polymorphic bands. The percentage of polymorphic bands was 96.8%. The coefficient ranges of interspecific genetic similarity (GS) in AFLP analyses for the 20 materials were 0.38-0.89. AFLP clustering results showed that the materials from the same habitats had a closer genetic relationship. There were abundant genetic diversity in species of Lycoris Herb. Conclusion: AFLP technique is efficient in generating more accurate information on the genetic diversity and relationship in plants of Lycoris Herb.