ABSTRACT
Background: \r\n', u'In the antituberculosis chemotherapy, shortening treatment course, and applying new high effective methods are top prioritized. Of which,the immunotherapy with M vaccea an environmental saprophyte, combined with the antituberculosis medicines all over the world has been applied since 1985, aimed to enhance the host immune responses.\r\n', u'Objectives:\r\n', u'To evaluate the results after 6 months of shortened course anittuberculosis chemotherapy by combining the immunotherapy withM vaccae.\r\n', u'Subjects and method: \r\n', u'233 newly diagnosed pulmonary tuberculosis patients with AFB smear (+) selected from Centers for Tuberculosis Control in some districts of Ha Tay and Ha Noi. They are 15 above, and not the pregnant, breastfeeding, diabetes, HIV infections, and hepatitis B (Unnecessary) \r\n', u'Dividing randomly into 2 groups, group A was in treatment of the standard 8 month chemotherapy regimen, and group B was treated in a shortned 6 month chemotherapy regimen in combination with immunotherapy by using M. vaccae vaccine. \r\n', u'Results:\r\n', u'The rates of AFB (-) sputum of group B were 98,2% and 100% respectively, the rates of the group A were 97,4% and 99% respectively after 2 and 6 months of treatment. There are 88.5% and 100% for group B, 85.6% and 98.8% for group A at the same time to achieve a negative sputum culture for M. tuberculosis. IgM responses against M. tuberculosis sonicated antigen before and after the follow-up time in Group A are not changed, whereas this level of the immunotherapy group had reduced significantly at the 6th moth (P<0.05). There is no responses in IgC to M. tuberculosis of both groups during the same follow-up period. The mean weight of patients in group B was higher than that of group A after 2 and 6 months of treatment.\r\n', u'Conclusion:\r\n', u'M. vaccae vaccine brings good benefits when be combined with the chemo-immunotherapy for newly diagnosed pulmonary tuberculosis patients with AFB smear (+). The negative sputum indicators and clinic improvements in the chemo- immunotherapy group of treatment are better than that of chemotherapy only. This study created more effective method for treating tuberculosis infection.\r\n', u'
Subject(s)
Tuberculosis , Tuberculosis , Mycobacterium tuberculosisABSTRACT
In order to enhance the capability of antiserum production rabbits, Mycobacterium vaccae was used to stimulate rabbit’s immune system unspecifically before immunization with Haemophilus influenzae type b (Hib). The results showed that only single immunization with Hib antigen: the number of rabbits had response fulfill depended-time requirements (harvested after 1 or 6 months) varied from 30% to 70%, harvesting time for antiserum with fulfilled quantity and quality was 24 weeks (6 months) (2 courses induced immunization). Stimulating rabbit’s immune system by M. vaccae, then inducing rabbit’s immunization with Hib antigen: the number of rabbits had response fulfiling requirements varied from 71.4% to 100%; harvesting time for antiserum with fulfilled quantity and quality was 5 weeks (only after 1 course induced immunization). Sensitivity and specificity of antiserum were unchanged
Subject(s)
Mycobacterium , Helicobacter pylori , Immune Sera , Adjuvants, ImmunologicABSTRACT
AIM: To explore the immunotheraptical drugs for multi-drug resistant pulmonary tuberculosis (MDR-TB). METHODS: 79 patients with MDR-TB were randomly assigned to two groups. 46 cases in M.vaccae group were treated with "3AkPaThLevL/15PaThLevL" and M. vaccae, and 33 cases in control group were treated only with "3AkPaThLevL/15PaThLevL". The clinical effect and T-lymphocyte subsets in patients were observed after being treated for 1,2 and 3 months, respectively. RESULTS: The sputum negative rates ((41.3)%, (63.0)%, and (80.4)%) and the X-ray resolution rates ((30.4)%, (50.0)%, and (67.4)%) of the M.vaccae group were superior to those ((12.1)%, (27.3)%, and (39.4)%, P
ABSTRACT
AIM: To study the effects of M. vaccae on eosinophil apoptosis and Bcl-2 protein expression in lung tissues of asthmatic guinea pigs. METHODS: 30 guinea pigs were divided into normal saline (NS) group, asthma group and M. vaccae treatment group at random, every group included 10 guinea pigs. Guinea pigs in M. vaccae treatment group were injected intramuscularly with 22 5 ?g M. vaccae 10 days before OVA immunization. TdT-mediated dUTP nick end labeling (TUNEL) technique was used to investigate the apoptosis of eosinophils and immunohistochemistry method was used to study the expression of Bcl-2 protein in lung tissues. RESULTS: The apoptosis index (AI) of eosinophils in lung tissues in M. vaccae treatment group was significant higher than that in asthma group (23 78?5 42)% vs (4 56?0 68)%, P