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Objective: To investigate the effect of total glycosides from Ranunculus japonicus (TGRJ) on vasculogenic mimicry of human gastric cancer MGC803 cell line in vitro and its mechanisms. Methods: The effect of TGRJ on the cell adhesion of MGC803 was observed by cell attachment assay. The effect of TGRJ on the cell migration of MGC803 was studied by cell wound healing assay. The function of TGRJ on the tube-like structures formation of MGC803 in vitro was verified by tube formation assay. The effect of TGRJ on the expression of vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. Results: TGRJ effectively inhibited the cell adhesion, cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry in vitro in a dosage-dependent manner. Furthermore, the semi-quantitative RT-PCR showed that TGRJ markedly inhibited the expression of MGC803 vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5. Conclusion: TGRJ could effectively inhibit the cell attachment and cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry by down regulating vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5.
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Objective To investigate the proliferation inhibition and apoptosis induction effect of thalidomide(Thal) and its joint effect with 5-Fu on MGC-803 cell line. Methods The morphological changes of MGC-803 cells with AO/EB stain were observed under fluorescence microscope. The proliferation inhibition effeet was evaluated with MTT. The apoptosis induction effect was determined by FAM. Results The results of MTr array were as below: the difference was significant between Thai groups of 25 mg/L or above concentration and the control group (P＜0.05), 5-Fu of all testing concentrations showed significant difference compared with the control gToup(P＜0.01). For combined groups with the same concentration of 25 mg/L 5-Fu,combined groups showed distinct difference from the corresponding 5-Fu group (P＜0.01), but two combined groups showed no distinct difference with each other (P＞0.05). For combined groups with the same concentration of 5-Fu 12.5 mg/L, 5-Fu plus 50 mg/L or 100 mg/L Thai showed no distinct difference from 5-Fu group of 25 mg/L(P＞0.05). With FAM study, all test groups showed significant difference compared with the control group (P＜0.01). There was also significant difference between two test groups arbitrarily (P＜0.01).Conclusion Both Thai and 5-Fu could inhibit the proliferation and induce the apoptosis of MGC-803 cells.The effect is reinforced with the combination significantly. Combination with Thai could decrease the concentration of 5-Fu but have the similar effect.
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Aim To investigate whether DATS induce MGC803 cell apoptosis and the relationship betweenapoptosis and Ca~(2+) disruption. Methods MGC-803 cell growth inhibition was measured by MTT assay. Tunnel and flow cytometry methods were used to determine the induction of apoptosis and Ca2+ homeostasis disruption. Result MTT assay showed that the inhibitory rates on MGC-803 cell growth of different concentrations of DATS 4,8,12,16 and 24 mg?L-1 were 0.231?0.037,0.305?0.036,0.455?0.029,0.607?0.058,0.751?0.019 respectively. Flow cytometry analysis showed that treating MGC803 cell with DATS significantly increased the percentage of apoptosis cells and intracellular Ca2+. Treatment of cells with 1,2-bis(2-aminophenoxye-thane)-N,N,N-tetraacetic acid tetrakis acetoxymethyl ester (BAPTA-AM), cellular Ca2+ chelator, resulted in abolishment of the elevation of intracellular Ca~(2+) and blockage of DATS induced apoptotic of MGC-803. Conclusoin DATS could induce apoptosis of MGC-803 cells through the mechanism of Ca~(2+) homeostasis disruption.
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AIM: To study the effects of growth inhibition of different concentrations of diallyl trisulfide(DATS) on gastric cancer MGC-803 cell line in vitro. METHODS: The influence of different concentrations of DATS were examined by MTT assay, clonal formation rates and cell growth curve. RESULTS: Suppression and decrease of MGC-803 cell proliferation was found after treatment by DATS in vitro. The inhibitory rates on MGC-803 cell growth of different concentration of DATS, 4, 8, 12, 16 and 24 mg·L-1, were 26%, 46%, 65%, 76% and 89% respectively, and its half inhibitory concentration (IC50) was 8.2 mg·L-1. The clonal formation rates and clonal formation relative counts of 8, 12, 16 and 24 mg·L-1 were 32.4% and 58.7%, 24.8% and 42.5%, 19.0% and 33.5%, 8.8% and 15.1% respectively. There was significant correlation between dose and effect in all, and the cell growth culve became lower and flatter when concentration of DATS increase gradually. CONCLUSION: The effect of growth inhibition of DATS for gastric cancer MGC-803 cell in vitro is remarkable.
ABSTRACT
AIM To study the effects of growth inhibition of different concentrations of diallyl trisulfide(DATS) on gastric cancer MGC 803 cell line in vitro. METHODS The influence of different concentrations of DATS were examined by MTT assay, clonal formation rates and cell growth curve. RESULTS Suppression and decrease of MGC 803 cell proliferation was found after treatment by DATS in vitro. The inhibitory rates on MGC 803 cell growth of different concentration of DATS,4, 8, 12, 16 and 24 mg?L -1 , were 26%,46%,65%,76% and 89% respectively, and its half inhibitory concentration (IC 50 ) was 8 2 mg?L -1 . The clonal formation rates and clonal formation relative counts of 8, 12, 16 and 24 mg?L -1 were 32 4% and 58 7%,24 8% and 42 5%,19 0% and 33 5%?8 8% and 15 1% respectively.There was significant correlation between dose and effect in all, and the cell growth culve became lower and flatter when concentration of DATS increase gradually. CONCLUSION The effect of growth inhibition of DATS for gastric cancer MGC 803 cell in vitro is remarkable.