Protective Role of Pomegranate on kidney of Albino Rat Treated with Monosodium Glutamate

Background: Sodium mono glutamate (MSG), the sodium salt of glutamic acid, is a food flavoring agent that is widely used in many countries. Pomegranate is used as a traditional medication in numerous countries, it is planted in Asian countries, Mediterranean countries and the U.S.A. Aim of the work: The present study aimed to detect structural and functional changes in adult rat kidney tissue treated with sodium mono glutamate, and the possible protective effect of pomegranate on the kidney treated with MSG. Materials and Methods: This study was done by using 60 adult Wistar Albino rats of both sexes were divided into three equal groups: Group I (control group), Group II (sodium mono glutamate treated group), and Group III (combined MSG and pomegranate treated group) Doses were given once daily for 8 weeks every day. At the end of the treatment period, blood samples collected from each rat were used for measuring the values of urea and creatinine. Also animals of the different groups were sacrificed at the end of the experiment, quickly dissected and the kidneys were removed and stained with hematoxylin and eosin (H&E) for the histological examination by light microscopy, other tissue sections were evaluated using a transmission electron microscope. Both were used to examine the effect of sodium mono glutamate on cortex of the kidneys of albino rats ,compared with control group and the combined MSG and pomegranate group. Results: There was a major rise in blood urea level and blood creatinine level in sodium mono glutamate treated group in contrast to the control group. There was a significant reduction in blood urea level and blood creatinine level in combined sodium mono glutamate and pomegranate treated group in comparison to MSG treated group. Examination of kidney tissue of rats treated with sodium mono glutamate (Group. II) showed damaging changes of its structure. The glomerulus had markedly widened blood capillaries with thickened filtration membrane. The epithelial tubular cells had marked degenerative changes. Examination of rats kidney tissue treated with sodium mono glutamate and pomegranate (Group III) revealed improvement of the lesions in the glomeruli and renal tubules. Conclusion: Pomegranate protected the kidneys and restricted the histological and functional alterations caused by sodium mono glutamate, and thus, there is an advantage of usage of pomegranate with sodium mono glutamate.

Alterations in APC, BECN1, and TP53 gene expression levels in colon cancer cells caused by monosodium glutamate

Abstract Colorectal cancer (CRC) is a disease with high incidence worldwide. As of 2018, it is the second leading cause of cancer deaths in the world. In Saudi Arabia, the incidence of this disease has been increasing in the younger population. Both genetic and lifestyle factors may have contributed to its increased incidence and pathogenesis. Monosodium glutamate (MSG) is a food flavor enhancer that can be found in many commercial foods, and it can sometimes be used as a substitute to table salt. MSG has been investigated for its possible genotoxicity, yielding controversial results. In the present study, the effect of MSG on cell viability and its effect on expression of APC, BECN1, and TP53 genes in SW620 and SW480 colon cancer cell lines were studied. TP53 is a tumor suppressor gene that functions in modifying DNA errors and/or inducing apoptosis of damaged cells, and both APC and BECN1 genes are involved in CRC and are of importance in cellular growth and metastasis. Cancer cell viability was analyzed using MTT assay, and the results showed a significant increase in the number of viable cells after 24 h of treatment with MSG with different concentrations (0.5, 1.0, 10, 50, and 100mM). Moreover, gene expression results showed a significant increase in the expression levels of APC and BECN1 under specified conditions in both cell lines; conversely, TP53 showed a significant decrease in expression in SW620 cells. Thus, it can be concluded that MSG possibly confers a pro-proliferative effect on CRC cells.

Resumo O câncer colorretal (CCR) é uma doença com alta incidência mundial. Desde 2018, é a segunda principal causa de mortes por câncer no mundo. Na Arábia Saudita, a incidência dessa doença vem aumentando na população mais jovem. Tanto fatores genéticos quanto de estilo de vida podem ter contribuído para o aumento da sua incidência e patogênese. O glutamato monossódico (MSG) é um intensificador de sabor de alimentos que pode ser encontrado em muitos alimentos comerciais e às vezes pode ser usado como um substituto do sal de cozinha. O MSG tem sido investigado por sua possível genotoxicidade, produzindo resultados controversos. Neste estudo, foram estudados o efeito do MSG na viabilidade celular e seu efeito na expressão dos genes APC, BECN1 e TP53 em linhas de células de câncer de cólon SW620 e SW480. TP53 é um gene supressor de tumor que atua modificando erros de DNA e/ou induzindo apoptose de células danificadas, estando os genes APC e BECN1 envolvidos no CRC e sendo importantes no crescimento celular e metástase. A viabilidade das células cancerosas foi analisada por meio do ensaio MTT, e os resultados mostraram um aumento significativo no número de células viáveis após 24 h de tratamento com MSG em diferentes concentrações (0,5; 1,0; 10; 50 e 100mM). Além disso, os resultados da expressão gênica mostraram um aumento significativo nos níveis de expressão de APC e BECN1 sob condições especificadas em ambas as linhagens celulares. Por outro lado, TP53 mostrou uma diminuição significativa na expressão em células SW620. Assim, pode-se concluir que, possivelmente, o MSG confere um efeito pró-proliferativo às células CRC.

Alterations in APC, BECN1, and TP53 gene expression levels in colon cancer cells caused by monosodium glutamate / Alterações nos níveis de expressão gênica APC, BECN1 e TP53 em células de câncer de cólon causadas por glutamato monossódico

Colorectal cancer (CRC) is a disease with high incidence worldwide. As of 2018, it is the second leading cause of cancer deaths in the world. In Saudi Arabia, the incidence of this disease has been increasing in the younger population. Both genetic and lifestyle factors may have contributed to its increased incidence and pathogenesis. Monosodium glutamate (MSG) is a food flavor enhancer that can be found in many commercial foods, and it can sometimes be used as a substitute to table salt. MSG has been investigated for its possible genotoxicity, yielding controversial results. In the present study, the effect of MSG on cell viability and its effect on expression of APC, BECN1, and TP53 genes in SW620 and SW480 colon cancer cell lines were studied. TP53 is a tumor suppressor gene that functions in modifying DNA errors and/or inducing apoptosis of damaged cells, and both APC and BECN1 genes are involved in CRC and are of importance in cellular growth and metastasis. Cancer cell viability was analyzed using MTT assay, and the results showed a significant increase in the number of viable cells after 24h of treatment with MSG with different concentrations (0.5, 1.0, 10, 50, and 100mM). Moreover, gene expression results showed a significant increase in the expression levels of APC and BECN1 under specified conditions in both cell lines; conversely, TP53 showed a significant decrease in expression in SW620 cells. Thus, it can be concluded that MSG possibly confers a pro-proliferative effect on CRC cells.

O câncer colorretal (CCR) é uma doença com alta incidência mundial. Desde 2018, é a segunda principal causa de mortes por câncer no mundo. Na Arábia Saudita, a incidência dessa doença vem aumentando na população mais jovem. Tanto fatores genéticos quanto de estilo de vida podem ter contribuído para o aumento da sua incidência e patogênese. O glutamato monossódico (MSG) é um intensificador de sabor de alimentos que pode ser encontrado em muitos alimentos comerciais e às vezes pode ser usado como um substituto do sal de cozinha. O MSG tem sido investigado por sua possível genotoxicidade, produzindo resultados controversos. Neste estudo, foram estudados o efeito do MSG na viabilidade celular e seu efeito na expressão dos genes APC, BECN1 e TP53 em linhas de células de câncer de cólon SW620 e SW480. TP53 é um gene supressor de tumor que atua modificando erros de DNA e/ou induzindo apoptose de células danificadas, estando os genes APC e BECN1 envolvidos no CRC e sendo importantes no crescimento celular e metástase. A viabilidade das células cancerosas foi analisada por meio do ensaio MTT, e os resultados mostraram um aumento significativo no número de células viáveis após 24 h de tratamento com MSG em diferentes concentrações (0,5; 1,0; 10; 50 e 100mM). Além disso, os resultados da expressão gênica mostraram um aumento significativo nos níveis de expressão de APC e BECN1 sob condições especificadas em ambas as linhagens celulares. Por outro lado, TP53 mostrou uma diminuição significativa na expressão em células SW620. Assim, pode-se concluir que, possivelmente, o MSG confere um efeito pró-proliferativo às células CRC.

Effect Of AJI-NO-MOTO On Kidney Of Adult Albino Rat: A Histopathological Evaluation

Introduction: Aji-No-Moto (MSG), the wonder flavoring agent, has been reportedly overused in all packed food products and the cuisines being served in restaurants. This salt effect almost all the organs of the body but the evidences regarding its ill effects are very limited. Thus, no guidelines are there for the safe limits of Aji-No-Moto use. In current study we planned to analyses the histopathological effects of Aji-No-Moto on kidney. Methods: The study was conducted on 18 inbred adult albino rats of either sex. The rats of control group (C) received only standard diet with dis?lled water, low dose test group (T1) rats received 0.5mg/kg of MSG dissolved in dis?lled water and high dose test group (T2) rats received 1.5mg/kg of MSG dissolved in dis?lled water per orally for 28 days. A?er the experimental period, the rats were sacrificed to dissect out the renal ?ssue which was later subjected to histological processing and ?ssue sec?oning. Observa?ons: The kidney ?ssue sec?ons of the control group (C) revealed normal renal architecture consis?ng of cor?cal labyrinth and medulla. The cor?cal labyrinth consisted of glomeruli, PCT and DCT whereas the medulla consisted of the ascending and descending limbs of loop of henle and collec?ng ducts. On the other hand, renal sec?ons of low dose group (T1) showed focal shrinkage of renal glomerulus and widening of the Bowman’s space. Furthermore, these changes were more pronounced in high dose group (T2) along with hypercellularity of glomeruli, dilata?on, hyperaemia and conges?on in the intertubular cor?cal blood vessels and mononuclear cell infiltrate. Conclusion: Aji-No-Moto is the most widely used flavoring agent whose minimal dose for use has to be evaluated. The current study was planned to access the minimal low dose limit of MSG for use. The results of aforemen?oned study revealed that even small dose of 0.5mg/kg/day is capable of producing histopathological effects on kidney.

Analysis of Tooth Enamel Structure and Mechanical Properties in Rats Induced Mono Sodium Glutamate (MSG)

@#Introduction: Formation of enamel begins in intrauterine. The process is prone to disturbances, for example bad nutrition intake. Monosodium Glutamate (MSG) is a food additive that is added to meals to improve the taste. Unmeasured use can result in physical abnormalities, growth, and immune system disruption. This research aim of this study was to analyze MSG consumption on rats during gestation and gestation to lactation on enamel structure and mechanical properties in their first offspring. Methods: Three groups of male rats, aged 21 days, which were born from mice induced by MSG during gestation (group 1), during gestation to lactation (group 2) and those without MSG (group 3 as a negative control group). Monosodium Glutamate is given daily at the dose of 1.54 mg/gr (body weight/ BW) orally, which starts on the fifth day of gestation until partition (23 days) in the first group and until weaning time (44 days) in the second group. Analysis of the structure and properties of enamel was performed on the lower left first molar using scanning electron microscope (SEM) and Vikers microhardness test. Results: The average enamel hardness in MSG induced mice during gestation, gestation and lactation periods, and without MSG was 242.7 Vickers hardness (HV); 238.3 HV and 309.1 HV respectively, while the porosity in the enamel structure is 13,1909%, 18,147% and 7,039%. Conclusion: MSG intake in mice during gestation and gestation to lactation results in abnormalities in the structure of the enamel and its mechanical properties in offspring.

Biochemical Changes in the Malnourished Rats Serum and Liver Exposed to Dietary Monosodium Glutamate

Monosodium glutamate (MSG) is a flavor enhancer. Its toxicity in a malnourished state appears not to have been fully investigated. This study was carried out to determine the effects of MSG on malnourished rats. Rats were randomly assigned into four groups of five rats/group. Group 1 rats were fed with malnourished feed; Group 2 rats received malnourished feed with dosed 1.6 mg/g MSG per body weight; Group 3 rats were fed with normal feed and dosed 1.6 mg/g MSG per body weight and Group 4 rats served as the control group (normal healthy rats) and were fed with normal feed for 28 days. After 28 days, the rats were sacrificed with the liver harvested and blood samples collected. Results from the study showed that malnourished rats had significantly lower levels of oxidative stress biomarkers including, anti-oxidants compared with the control. The levels of malondialldehyde concentration and xanthine oxidase activity were high in malnourished fed rats. Aspartate aminotransferase and alanine transaminase levels of malnourished and normal rats administered MSG were significantly low compared to the normal healthy suggesting that labialization occurs in liver leading to leakage of these enzymes from the liver to the serum. Malnourished rats showed significant decrease in body weight losing 48 grams after 28 days compared to malnourished and normal rats fed with MSG which recorded significant increase in body weight after 28 days adding 26 g and 42 g respectively.

Hepatoprotective Potentials of Ethanolic Extract of Solanum melongena Linn Fruit on Monosodium Glutamate- Intoxicated Rats' and Liver Section Histology

This study evaluated the hepatoprotective potentials of ethanolic extract of Solanum melongena Linn fruit on monosodium glutamate-intoxicated rats and liver section histology using standard protocols. Twenty four Wistar rats that weighed 105.00 ± 7.00 g were divided into 6 groups of 4 each. The six groups were thus fed: Group 1 (control, feed and distilled water only), Group 2 (8000 mg/kg body weight MSG), Group 3 (300 mg/kg body weight the sample extract), Group 4 (8000 mg/kg body weight MSG+ 100 mg/kg body weight the sample extract), Group 5 (8000 mg/kg body weight MSG+ 300 mg/kg body weight the sample extract) and Group 6 (8000 mg/kg body weight MSG+ 500 mg/kg body weight the sample extract) daily for 14 days. The MSG only fed group showed a significant (P=0.05) increase in the activities of serum alanine aminotransferases (ALT), aspartate aminotransferases (AST), alkaline phosphatases (ALP) and total bilirubin compared to the control group. MSG and ethanolic extract of Solanum melongena Linn fruit co-treated groups at different concentration were significantly (P=.05) reduced to become comparable to the control group. The concentration of serum albumin and total protein were significantly (P=.05) reduced for group 2 compared to group 1. The co-administration of MSG and ethanolic extract of Solanum melongena Linn fruit resulted to a significant (P =0.05) increase notably at 500 mg/kg body weight sample extract and the same was observed for the histology. This suggests the improved synthetic function of the liver. MSG at a high concentration (8000 mg/kg body weight) adversely affected the rats liver biofunction and the hepatoprotective potentials of ethanol extract of Solanum melongena Linn fruit was notably at 500 mg/kg of body weight in rats. However, further studies in this direction are warranted.

Biochemical and Histological Effects of Low Dose of Monosodium Glutamate on the Liver of Adult Male Sprague-Dawley Rats / Jurnal Sains Kesihatan Malaysia

@#Monosodium glutamate (MSG) is widely used as an additive in food. Excess consumption of MSG was reported to cause oxidative stress on brain, liver and renal resulted in increased production of reactive oxygen species (ROS). This study aims to determine the biochemical and histological effects of low dose MSG on the liver of adult male Sprague-Dawley rats. Animals (n=6 per group) were randomly divided into three groups with two treatment groups: 60mg/kg (MSG60) and 120mg/kg (MSG120), and one control group (distilled water). The substances were administered to the rats via force feeding for 28 consecutive days. On day 29, all rats were killed, and liver tissues were biopsied for the biochemical (total protein, liver enzymes, and the status of oxidative stress) and histological analysis. The total protein appeared significantly decreased (p<0.05) while alanine aminotransferase (ALT) and aspartate aminotransferase (AST) demonstrated a significant increased (p<0.05) in the MSG120 treatment group as compared to the control group. Malondialdehyde (MDA) levels and the antioxidant levels of superoxide dismutase (SOD) were significantly increase (p<0.05) in the MSG120 group as compared to the MSG60 and control groups. The histological findings revealed changes to normal liver architecture and accumulation of red blood cells in the central veins in both MSG groups. This study indicates that the MSG consumption at a dose of 120 mg/kg may alter the biochemical and histological parameters of the liver.

1,25-Dihydroxyvitamin D3 protects obese rats from metabolic syndrome promoting regulatory T cell-mediated resolution of inflammation

Vitamin D has been found to produce therapeutic effects on obesity-associated insulin resistance and dyslipidemia through its potent anti-inflammatory activity, but the precise immunomodulatory mechanism remains poorly understood. In the present study we found that 1,25-dihydroxyvitamin D [1,25(OH)D], the biologically active form of vitamin D, significantly attenuated monosodium glutamate (MSG)-induced obesity and insulin resistance as indicated by body weight reduction, oral glucose tolerance improvement, and a glucose infusion rate increase as detected with hyperinsulinemic-euglycemic clamp. Moreover, 1,25(OH)D not only restored pancreatic islet functions but also improved lipid metabolism in insulin-targeted tissues. The protective effects of 1,25(OH)D on glycolipid metabolism were attributed to its ability to inhibit an obesity-activated inflammatory response in insulin secretory and targeted tissues, as indicated by reduced infiltration of macrophages in pancreas islets and adipose tissue while enhancing the expression of in liver tissue, which was accompanied by increased infiltration of Treg cells in immune organs such as spleen and lymph node as well as in insulin-targeted tissues such as liver, adipose, and muscle. Together, our findings suggest that 1,25(OH)D serves as a beneficial immunomodulator for the prevention and treatment of obesity or metabolic syndrome through its anti-inflammatory effects.

Evaluation of the Changes in Some Liver Function and Haematological Parameters in MSG Fed Rats.

The effect of Monosodium glutamate (MSG) was evaluated on hepatic functions and haematological parameters in adult Wistar albino rats. The rats were randomly assigned into four (4) groups of five rats each. Group 1 served as the control while groups 2, 3 and 4 were fed diets supplemented with MSG at doses of 0.5, 1.0 and 5.0% per Kg of diet respectively. Following the 28 days of feeding, the rats were sacrificed and blood samples were collected for analyses. The haematological parameters except PCV were estimated using haematocytometer while the biochemical parameters were determined using Randox enzymatic kit. There was no significant difference (p>0.05) in PCV, WBC, RBC, total protein, albumin, direct and conjugated bilirubin in the rats fed with MSG at all levels of supplementation when compared with the control group. The Alkaline phosphatase (ALP) and Alanine aminotransferase (ALT) activities were increased with increase level of MSG and ranged from 35.00±0.17 - 59.00±1.23 U/L and 6.4-11.9 U/L respectively. There was no significant (p>0.05) difference in the levels of both serum ALP and ALT activities in the group fed 0.5% MSG when compared with the control group while there were significant (p>0.05) increase in the other treatment groups (1 and 5% MSG supplemented diets groups). The results therefore suggest that MSG at the levels of supplementation in the diets of the rats had no effect on the haematological indices but increased the liver function enzymes in the serum as the level of MSG increased in the diet.

Preparation and quality control of methyl salicylate glycoside tablets / 中国药学杂志

OBJECTIVE: To establish the method of the preparation and test for methyl salicylate glycoside (MSG) tablets. METHODS: MSG tablets were prepared with MSG as main drug and microcrystalline cellulose and sodium carboxymethyl starch as exipients. Its detection method was studied. RESULTS: After experimental study, the high performance liquid chromatography method for related substances detection and content determination of MSG tablets were established. CONCLUSION: The prescription is reasonable and the preparation technology is reliable. The HPLC method has high accuracy and sensitivity and can be used for MSG tablets test.

Chronic Administration of Monosodium Glutamate under Chronic Variable Stress Impaired Hypothalamic-Pituitary-Adrenal Axis Function in Rats

The hypothalamic-pituitary-adrenal (HPA) axis is the primary endocrine system to respond to stress. The HPA axis may be affected by increased level of corticotrophin-releasing factors under chronic stress and by chronic administration of monosodium glutamate (MSG). The purpose of this study was to investigate whether chronic MSG administration aggravates chronic variable stress (CVS)-induced behavioral and hormonal changes. Twenty-four adult male Sprague-Dawley rats, weighing 200~220 g, were divided into 4 groups as follows: water administration (CON), MSG (3 g/kg) administration (MSG), CVS, and CVS with MSG (3 g/kg) administration (CVS+MSG). In addition, for the purpose of comparing the effect on plasma corticosterone levels between chronic stress and daily care or acute stress, 2 groups were added at the end of the experiment; the 2 new groups were as follows: naive mice (n=7) and mice exposed to restraint stress for 2 h just before decapitation (A-Str, n=7). In an open field test performed after the experiment, the CVS+MSG group significant decrease in activity. The increase in relative adrenal weights in the CVS and CVS+MSG group was significantly greater than those in the CON and/or MSG groups. In spite of the increase in the relative adrenal weight, there was a significant decrease in the plasma corticosterone levels in the CVS+MSG group as compared to all other groups, except the naive group. These results suggest that impaired HPA axis function as well as the decrease in the behavioral activity in adult rats can be induced by chronic MSG administration under CVS rather than CVS alone.

Attenuated Sympathetic Activity and Its Relation to Obesity in MSG Injected and Sympathectomized Rats

In order to characterize the role of sympathetic activity in obesity, we repeatedly assessed sympathetic activity via power spectral analyses of heart rate variability in the same subjects at 7, 11, 25, and 60 weeks, using monosodium glutamate (MSG)-induced obese and control rats. The effects of lower sympathetic activity on obesity were also evaluated. Fat mass in MSG rats was already higher at 7 weeks, but the sympathetic activity did not differ between 7 and 25 weeks. Between 25 and 60 weeks, the increase in fat mass, food efficiency, and body weight gain was higher in MSG rats. The increase in sympathetic activity between 25 and 60 weeks and sympathetic activity at 60 weeks were lower in MSG rats. Fat mass at 60 weeks was inversely correlated with changes in sympathetic activity between 25 and 60 weeks. Reduced plasma epinephrine levels by bilateral adrenal demedullation induced increase of fat mass. In summary, an attenuated increase of sympathetic activity with age may partly be responsible for aggravated obesity in MSG rats. Additionally, reduced sympathetic activity per se induced obesity in rats. These results suggest that lower sympathetic activity contributes to obesity in rats.

THE EFFECT OF MSG ON THE EXPRESSION OF 5-HT IN GASTROENTERIC MUCOSA OF RAT / 解剖学报

Objective To explore the effect of monosodium glutamate (MSG) on the expression of 5\|HT in gastroenteric mucosa of rat. Methods Immunohistochemical technique was used to detect the expression of 5\|HT. The immuno\|activity and the density of positive cells were measured by image analysis. Results The immuno\|activity and the density of 5\|HT positive cells(EC cell) in the experimental group are higher than that in control group. The effect by 120d is the most significant, followed by that of 52d. Statistical analysis showed a significant difference ( P

The Effects on the MSG with Phenylalanine Treatment in the Area Postrema of the Rat Medulla / 체질인류학회지

Glutamate is an amino acid neurotransmitter capable of producing widespread receptor-mediated neuronal excitation. In this experiment, we examined the effect of saline, monosodium glutamate (MSG), phenylalanine and MSG-phenylalanine treatment on TH immunoreactivity in area postrema (AP) of medulla oblangata. An immunocytochemical method was used to visualize catecholaminergic neurons in the AP. Damage of TH neurons in the AP of adult Sprague-Dawley rats was induced by injection of MSG (4 mg/g bw) and was decreased by administration of MSG following phenylalanine treatment (15 mg/g bw). We conclude that phenylalanine protect from the neuroexcitotoxic effect of systemic glutamate.

Preliminary study of insulin resistance induced by neonatal monosodium glutamate treatment in normal Wistar rats / 中国药理学通报

AIM To study the glucose and lipids metabolism and insulin sensitivity of MSG rats during their growing period, and to evaluate the effects of insulin sensitizer pioglitazone on the model rats. METHODS Body weights were measured regularly, and glucose and insulin tolerance tests were taken. In their 3 and 10 months old, rats were given insulin sensitizer pioglitazone orally, then the effects on serum glucose, triglyceride, cholesteral, free fatty acid and insulin concentrations were determined. RESULTS Compared with normal rats, a slight but significant increase of glucose in MSG rats was revealed. The serum triglyceride, cholesteral, free fatty acid and insulin concentrations were significantly higher in model rats. Moreover, gluconeogenesis increased significantly, and insulin tolerance showed abnormal. However, glucose tolerance was nearlly normal. Pioglitazone could ameliorate all these metabolic disorders. CONCLUSION Obesity and insulin resistance were induced by injecting monosodi- um glutamate (MSG) to neonatal Wistar rats. Piogli- tazone can significantly improve the insulin sensitivity of Msc rats. These results suggested that MSG obese rats can be used as an easily accessible and inexpensive insulin resistance animal model for evaluating the efficacy and mechanisms of antidiabetic agents.

Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii major surface glycoprotein gene's upstream conserved sequence / 重庆医科大学学报

Objective:To construct and identify a plasmid vector of short interfering RNA(siRNA)on pneumocystis carinii(PC) major surface glycoprotein(MSG)gene's upstream conserved sequence(UCS).Methods:Short hairpin RNA(shRNA) oligonucleotide targeting PC MSG UCS gene which was chemically synthesized and inserted into RNAi-Ready plasmid vector pTZU6+1 after annealing.The recombinant plasmid,PC-UCS,transformed into E.coil.TOP10 and amplified,was digested by restriction endonucleases HindⅢand EcoRI and identified by gel electrophoresis and DNA sequencing.Results:Gel electrophoresis and DNA sequencing showed that the recombinant plasmid containing the correct and full shRNA oligonucleotide. Conclusion:The siRNA plasmid,pPC-UCS,was constructed successfully.