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Medical abortions have been a national as well as social concern for the society, especially in India. There have been certain laws, acts and amendments that have been designed to promote safe abortions. Despite, the rules in place, there is still need to have more awareness as the women still face difficulties in accessing abortion services. The reasons may include legal hurdles, stigma, financial concerns and many more. Through this article, we would like to emphasize the role of government, medical professionals, health care providers to come together and form more dependable guidelines that may help women to access safer abortions.
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Reform of the abortion laws in favour of the well-being of pregnant women is one aspect of the removal of gender discrimination. The Medical Termination of Pregnancy Act (MTP Act) 1971, was a breakthrough legislation in this regard, as it reduced the number of unsafe illegal abortions. With advancements in ultrasonography and genetic technologies, many foetal malformations and genetic disorders were being diagnosed after 20 weeks of gestation. The fact that termination of pregnancy was not legally permitted beyond 20 weeks of gestation caused great distress to such women, and highlighted the need to increase the upper limit of termination of pregnancy. Concurrently, there has been greater awareness around the world on the rights of women to take decisions regarding their own bodies. The MTP Bill, 2020, has come as a breath of fresh air extending the term limit for legal abortions to 24 weeks for certain categories of women, and removing the limit for abortion in the presence of a significant foetal abnormality. The amendments were recently approved by Parliament and the President of India, and have become law as of March 25, 2021. This paper presents the amendments made and their implications for obstetric, ultrasonographic and foetal medical practice. It also presents a critique of the various Acts and suggests further amendments that would enhance the value of the Act.
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Background: Unsafe abortions continue to cause maternal morbidity and mortality in developing countries. The practice of unsafe abortions by quacks needs to be checked. Our study aims to emphasize upon the unmet needs of medical termination pregnancies (MTP) services in rural India and to recognize the complications due to it and the efficient management of such cases at tertiary care center.Methods: A two years retrospective study of septic abortions from December 2009 to November 2011.Results: Among 1080 abortions reported, 44 were septic-4.07%. More commonly in the age group of >20years (81.9%). 77.3% of them were multiparous and 22.7% were nulligravidae, with an increased incidence of unmarried nulliparous pregnancies. Greater numbers occurred during 1st trimester (77.2% versus 22.7%), with 54.5%-grade I, 29.5%-grade II, 15.9% grade III in severity. Majority of cases were due to evacuation by quacks (72.7%). Among the 44 cases, emergency laparotomy was done for 5 cases of grade III severity. The mortality rates due to septic abortion were 6.25% (3) among the total of 48 maternal deaths.Conclusions: Septic abortion is totally preventable. Majority of uneducated rural women are not aware of MTP services. The reproductive and child health (RCH) services should effectively reach the underprivileged population like slum dwellers and migrants.
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Aims@#Biofilm formation by Methicillin-resistant Staphylococcus aureus on a variety of surfaces and detection of the biofilm-forming population by the most reliable method is very much essential to diagnose the nosocomial infection caused by S. aureus. @*Methodology and results@#This study is aimed to evaluate the biofilm producing ability of S. aureus by qualitative Congo red agar (CRA), and quantitative microtitre plate (MTP) methods. The morphological difference of biofilms analysis was done by SEM (Scanning Electron Microscope) and genotyping analysis of mecA and femA for determination of MRSA among isolated S. aureus strains and to check the biofilm producers among MRSA strains. Biofilm production was found to be at different intensities by MTP. The strong, moderate and weak biofilm producers were found to be 38.63%, 31.81%, and 29.54% respectively. The strong adherent biofilm formed by representative isolate developed a dense biofilm with thick mucus three-dimensional multilayered structure of macroscopic dimension. Conversely, SEM analysis of moderate and weak biofilm representative strain failed to form a monolayer of scattered single cells to three-dimensional structure. The 47.72% of S. aureus isolates have shown positive for the genotypic analysis of mecA and femA. The strong and moderate biofilm forming MRSA was found to be 38.63% and 9.09%, respectively. @*Conclusion, significance and impact of study@#The great challenge is associated with biofilm mediated infection caused S. aureus healthy and hospitalized individual hence the present study reinforces the need of precautionary measures to avoid the indiscriminate use of antibiotics in case of biofilm-forming MRSA.
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Abstract@#Introduction: The practice of uncrossmatched red blood cells (URBC) transfusion has been accepted as part of resuscitation efforts in Emergency Departments (ED), especially in the case of hypovolemic shock secondary to blood loss. This study aimed to evaluate the outcome of patients who received URBC during the resuscitation process. Methods: This was a retrospective cross-sectional study among patients who received at least one unit of URBC in the ED at Hospital Universiti Sains Malaysia between March 1, 2016 and December 31, 2017. The outcome of patients who received URBC were analysed descriptively and presented as numbers and percentages. Simple log regression was used to analyse the association between the number of URBC given and the outcome of the patients. Results: A total of 106 patients were involved in this study, with 63 (59.4%) of them were male and 43 (40.6%) were female. The mean age of these patients was 41.58 years old. For the outcome, 25 patients (23.6%) had an activation of massive transfusion protocol (MTP), 37 patients (34.9%) received emergency surgical procedure, and 28 patients (26.4%) died during hospitalisation. The mean length of hospital stay was 10.65 days (SD: 17.4). Numbers of URBC received have a statistically significant association with mortality status (p = 0.015) and activation of MTP (p = 0.02), but no significant association with the need for emergency procedure (p = 0.469). Conclusion: The numbers of URBC transfused can be associated with mortality and the activation of MTP.
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OBJECTIVE:To investigate the effects of MTP gene polymorphism on the lipid-regulating effect of simvastatin in the treatment of T2DM complicating with lipid metabolism disorder. METHODS:120 T2DM inpatients with hypercholesterolemia were selected from our hospital during Jun. to Dec. 2015 and given Metformin hydrochloride sustained-release tablets,Enalapril ma-leate tablets and Simvastatin tablets. PCR-RFLP was used to detect MTP G493T genotype. The levels of TG,TC,LDL-C and HDL-C were detected by AU 400 automatic biochemistry analyzer before treatment and the 4th week after treatment. The effects of different genotype on the change of blood lipid level was analyzed. RESULTS:Among 120 patients,the patients with MTP G493T GG,GT,TT genotypes accounted for 61.67%,26.67% and 11.67%,respectively,meeting Hardy-Weinberg balance(P>0.05). Before treatment,there was no statistical significance in TC,TG,LDL-C and HDL-C among different genotypes(P>0.05). After 4 weeks of treatment,the decrease of TC and LDL-C in TT genotype patients were lower than that in GG and GT genotype,with statistical significance(P>0.05). There was no significant difference in the decrease of TC and LDL-C between GT and GG geno-type,the decrease of TG and HDL-C(P>0.05). There was no significant difference in the incidence of ADR among different geno-types(P>0.05). CONCLUSIONS:After simvastatin treatment,the improvement of TC and LDL-C in patients with TT genotype is poor. MTP G493T gene polymorphism may be associated with the lipid-regulating effect of simvastatin in the treatment of T2DM patients with lipid metabolism disorder.
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Background: Unplanned and unwanted pregnancies are common occurrences in all societies, regardless of the level of medical, economic, educational or religious development present within them. Despite wider availability of contraceptive methods, the incidence of induced abortion is increasing. Aim: To study the efficacy of Mifepristone 200 mg orally followed 48 hours later by Misoprostol 600 µg per vaginally in women undergoing medical termination of early pregnancy (up to 73 days of gestational age). Materials and methods: The present study included 50 pregnant women requesting termination of pregnancy in first trimester attending the department of Obstetrics and Gynecology. Women with gestational age up to 73 days from the first day of the last menstrual period with previous regular cycles were studied. Patients without medical or surgical contraindications to Mifepristone and Misoprostol were included. Women without prior caesarean section were studied. Results: Majority of the patients were of age group between 21-25 years. 92% of these women were married, 15% were nulliparous and 35% were multiparous. The period of gestation varied from 38-73 days. Majority of women were between 36-50 days of gestational age, constituting 50%. 94% of the patients had complete abortion and 4% had incomplete abortion. Success rate was not affected by the parity or gestational age, 72% of the patients expelled the products of conception within 10 hours, 84% in 15 hours and almost all the patients within 24 hours of prostaglandin administration. Most of the adverse effects reported were of the gastrointestinal system of which nausea was reported by 40%, abdominal pain by 70%, vomiting by 16% and diarrhoea by 4% of the cases. 6% of the patients reported excess bleeding at the time of abortion but none of them required hospitalization or blood P. Yamini Shobha Vani, A. Niranjani Devi. Efficacy of Mifepristone and Misoprostol combination in termination of early pregnancy. IAIM, 2016; 3(12): 48-54. Page 49 transfusion. The mean duration of bleeding was 7.24 days. A significant fall in hemoglobin was not observed in any patient (less than 1 gm/dl). This combination has the advantage of high complete abortion rate with low frequency of side effects. Conclusion: The combination of RU 486 200mg orally and misoprostol 600 µg intravaginally appears to offer safe, efficient, acceptable, out-patient procedure and an alternative to surgical abortion in early termination of pregnancy (up to 73 days of gestation).
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Background: Maternal mortality is an important indicator of women’s health and social well-being. According to the WHO report, each year, an estimated 190 women for every 100,000 live births die due to maternal causes. In India, unsafe abortion and related complications accounts for 9% of all maternal deaths. Abortion was made legal in India by the Medical Termination of Pregnancy (MTP) act, which was enacted by Indian Parliament in the year 1971. But the number of unsafe abortions has not declined. Methods: A present study was conducted by the Department of Obstetrics and Gynaecology of Pondicherry Institute of Medical Sciences, in Puducherry, India. All undergraduate students of VIIth semester MBBS participated in the study. A pre-designed semi-structured questionnaire was used to collect information regarding the awareness and perceptions on abortion care and Medical Termination of Pregnancy Act in India. Data were entered in Microsoft Excel 2007. Averages were calculated and chi square test was applied to find the significant difference. Results: Among a total of 75 participants, 41(54.7%) were female and 34 (45.3%) were male students. Majority of the students (97.3%) were aware of the fact that unsafe abortions are a serious health problem in India and all of the students (100.0%), were aware of the MTP Act of India. But only 36.0% students were aware of the fact, that consent from husband was not required to undergo abortion. To reduce the number of unsafe abortions in India majority of the medical students (45.3%) were of the opinion that easy access to MTP services in the community is an effective measure. Conclusions: Legalization of abortions through the MTP Act in India resulted in a considerable decrease in maternal mortality through the decline in abortions but it has failed to ensure effective implementation and access to medically safe abortion services. Training in basic contraceptive counseling and abortion care should be incorporated in basic medical education in India.
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Background: Medical termination of pregnancy is defined as “willful termination of pregnancy before the age of viability of the foetus. The objective was to study the socio-demographic & obstetric profile and the reasons for procuring an abortion & choice of health care provider. Methods: A Cross-sectional study was done in urban slum in Jamnagar municipal corporation area by using 30 clusters sampling technique. Every women in the reproductive age group (15-49 years) was included in the study till the sample size of 15 was completed in each cluster and thus total 450 women were interviewed. 48 women out of the total of 450 ever married women in the reproductive age group who had ever undergone induced abortion were studied in detail Results: In the present study out of 450 eligible women, 48 (10.67%) had undergone an induced abortion and once only. 72.91% (35/48) of the women belonged to the age group 25-34 years and 89.59% (43/48) of the women were Hindus. Almost half of the total women (45.83%) had primary education, higher proportion of women were housewife 77.09% (37/48). According to Modified Prasad’s classification, 54.17 % of women belonged to lower social class (IV and V). One third (33.33%) of the women who underwent MTP had 1 living child. Majority 79.16% of the women preferred government hospital and in 45.83% cases both husband and wife acted as decision maker for MTP. Most common reason given for terminating the pregnancy was “birth spacing”. (52.09%) Other reasons given were, “family completed” (22.91%), “medical” (14.58%) & social (4.17%). Majority of the women (70.83%) accepted post abortion contraception. Conclusion: There is need to counsel women of reproductive age group that MTP is not a way to control unwanted birth and it is not free from risk. They should be motivated for various methods of contraception.
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Statins have been shown to be very effective and safe in numerous randomized clinical trials, and became the implacable first-line treatment against atherogenic dyslipidemia. However, even with optimal statin treatment, 60% to 80% of residual cardiovascular risk still exists. The patients with familial hypercholesterolemia which results in extremely high level of low density lipoprotein cholesterol (LDL-C) level and the patients who are intolerant or unresponsive to statins are the other hurdles of statin treatment. Recently, new classes of lipid-lowering drugs have been developed and some of them are available for the clinical practice. The pro-protein convertase subtilisin/kexintype 9 (PCSK9) inhibitor increases the expression of low density lipoprotein (LDL) receptor in hepatocytes by enhancing LDL receptor recycling. The microsomal triglyceride transport protein (MTP) inhibitor and antisense oligonucleotide against apolipoprotein B (ApoB) reduce the ApoB containing lipoprotein by blocking the hepatic very low density lipoprotein synthesis pathway. The apolipoprotein A1 (ApoA1) mimetics pursuing the beneficial effect of high density lipoprotein cholesterol and can reverse the course of atherosclerosis. ApoA1 mimetics had many controversial clinical data and need more validation in humans. The PCSK9 inhibitor recently showed promising results of significant LDL-C lowering in familial hypercholesterolemia (FH) patients from the long-term phase III trials. The MTP inhibitor and antisesnse oligonucleotide against ApoB were approved for the treatment of homozygous FH but still needs more consolidated evidences about hepatic safety such as hepatosteatosis. We would discuss the benefits and concerns of these new lipid-lowering drugs anticipating additional benefits beyond statin treatment.
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Humans , Apolipoprotein A-I , Apolipoproteins , Apolipoproteins B , Atherosclerosis , Cholesterol, HDL , Cholesterol, LDL , Dyslipidemias , Hepatocytes , Hydroxymethylglutaryl-CoA Reductase Inhibitors , Hyperlipoproteinemia Type II , Lipoproteins , Receptors, LDL , Recycling , TriglyceridesABSTRACT
Radial longitudinal deficiency, also known as radial club hand, is a congenital deformity of the upper extremity which can present with a spectrum of upper limb deficiencies. The typical hand and forearm deformity in such cases consists of significant forearm shortening, radial deviation of the wrist and hypoplasia or absence of a thumb. Treatment goals focus on the creation of stable centralized and functionally hand, maintenance of a mobile and stable wrist and preservation of longitudinal forearm growth. Historically centralization procedures have been the most common treatment method for this condition; unfortunately centralization procedures are associated with a high recurrence rate and have the potential for injury to the distal ulnar physis resulting in a further decrease in forearm growth. Here we advocate for the use of a vascularized second metatarsophalangeal joint transfer for stabilization of the carpus and prevention of recurrent radial deformity and subluxation of the wrist. This technique was originally described by the senior author in 1992 and he has subsequently been performed in 24 cases with an average of 11-year follow-up. In this paper we present an overview of the technique and review the expected outcomes for this method of treatment of radial longitudinal deficiency.
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Humans , Forearm/abnormalities , Hand Deformities, Congenital/surgery , Joints/transplantation , Metatarsophalangeal Joint/surgery , Radius/abnormalitiesABSTRACT
Radial longitudinal deficiency, also known as radial club hand, is a congenital deformity of the upper extremity which can present with a spectrum of upper limb deficiencies. The typical hand and forearm deformity in such cases consists of significant forearm shortening, radial deviation of the wrist and hypoplasia or absence of a thumb. Treatment goals focus on the creation of stable centralized and functionally hand, maintenance of a mobile and stable wrist and preservation of longitudinal forearm growth. Historically centralization procedures have been the most common treatment method for this condition; unfortunately centralization procedures are associated with a high recurrence rate and have the potential for injury to the distal ulnar physis resulting in a further decrease in forearm growth. Here we advocate for the use of a vascularized second metatarsophalangeal joint transfer for stabilization of the carpus and prevention of recurrent radial deformity and subluxation of the wrist. This technique was originally described by the senior author in 1992 and he has subsequently been performed in 24 cases with an average of 11-year follow-up. In this paper we present an overview of the technique and review the expected outcomes for this method of treatment of radial longitudinal deficiency.
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Humans , Forearm/abnormalities , Hand Deformities, Congenital/surgery , Joints/transplantation , Metatarsophalangeal Joint/surgery , Radius/abnormalitiesABSTRACT
PURPOSE: The aim of this study was to investigate the radiological characteristics of the osteoarthritis of the second metatarsophalangeal (MTP) and tarsometatarsal (TMT) joint. MATERIALS AND METHODS: Between January 2002 and August 2010, 27 patients (33 feet) who had second metatarsal osteoarthritis (OA) were reviewed retrospectively. Group 1 was 14 patients (17 feet) with second MTP joint OA. Group 2 was 13 patients (16 feet) with second TMT joint OA. Group 3 was 24 patients (25 feet) had hallux valgus without second metatarsal (MT) OA as control. Weight bearing foot anteroposterior (AP) and lateral view were checked, and measured hallux valgus angle, metatarsus adductus angle (MAA), second MT functional length, first and second MT length by Hardy & Clapham method on AP view, angle of second MT with horizontal plane, calcaneal pitch, talo-first MT on lateral view. RESULTS: On weight bearing foot AP view, second MT functional length of group 1, 2, 3 was 2.4 mm, -0.1 mm, 0.7 mm and MAA of group 1, 2, 3 was 17.7degrees, 17.7degrees, 14.5degrees. Second MT functional length of group 1 was longer than control group and it was statistically significant. MAA was significant different between group 1-3 and group 2-3. Angle of second MT with horizontal plane of group 2 was smaller than control group and it was statistically significant. Other radiographic parameters have no statistical significance. CONCLUSION: Group 1 has long functional length of second MT and group 2 has small angle of second MT with horizontal plane.
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Humans , Foot , Hallux Valgus , Joints , Metatarsal Bones , Metatarsus , Osteoarthritis , Retrospective Studies , Weight-BearingABSTRACT
An orphan, mentally retarded woman, above 18 years age, when suffered pregnancy as a result of rape posses a serious challenge before the Chandigarh Administration on the issue of MTP. Law on abortion in India as per the MTP Act, 1971, (Amendment 2002) prohibits MTP without consent, if the woman is above 18 years of age. Several subject-experts to contend that with the advancement of Medical Science, universal recognition of the Fundamental Rights of the mentally retarded persons, recent theory of mixing them in the main social stream instead of barricading at a secluded place. The legislative transformation has also taken place whereby purposefully and knowingly, the competence to give consent for MTP in the cases of mentally ill pregnant woman on one hand and mentally retarded pregnant woman on the other hand, has now been distinguished. A critical review of prolife and prochoice support for abortion, statutory provisions in India and abroad, views of Hon’ble Court are discussed in detail. Paper tried to raise the debate for policy makers, higher judiciary and medical fraternity especially dealing with MTP.
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Os pontos gatilhos miofasciais (PGMs) são manifestações comumente encontradas na prática clínica e estão relacionados à alteração de tônus e à síndrome dolorosa miofascial (SDM). OBJETIVO: Avaliar a capacidade da EMG de superfície e detectar alterações da atividade neuromuscular no PGM, em situações de repouso e de contração isométrica voluntária máxima. MÉTODO: Participaram do estudo 56 indivíduos distribuídos em dois grupos: grupo Saudável com 28 indivíduos, que necessariamente não possuíam PGM, e o grupo Dor, constituído de 28 indivíduos que possuíam necessariamente PGM no músculo escolhido. O grupo Dor apresentava indivíduos com PGM latente e ativo, e com e sem fenômenos autonômicos (FA). RESULTADOS: O sinal EMG da porção muscular com PGM mostrou-se significativamente maior quando comparado com a porção muscular sadia do grupo Dor, e do grupo Saudável durante o repouso (26,56 ± 44,54, 5,39 ± 6,29 e 1,56 ± 0,76, respectivamente, p = 0,0001). Os indivíduos com PGM ativo obtiveram maior intensidade do sinal EMG do que aqueles que apresentaram PGM latente (17,85 ± 30,25 versus 3,74 ± 1,52, p = 0,04). Além disso, os indivíduos que apresentaram fenômenos autonômicos tiveram maior intensidade do sinal EMG do que aqueles que não os apresentaram (16,78 ± 28,44 versus 3,51 ± 3,65), na condição de repouso. CONLUSÃO: A EMG de superfície é capaz de mensurar a atividade do PGM, principalmente na condição de repouso.
There are examples of common clinical conditions that clinical signals are related to alterations in muscle tone, including myofascial pain syndrome. OBJECTIVE: to discuss the application of surface EMG to detect effect of miofascial trigger point (MTP) on neuromuscular activity at rest and maximum voluntary contraction of the trapezoid muscle. METHODS: Fifty-six subjects participated in the study and were divided into two groups: Healthy group (n = 28), with subjects who necessarily did not present MTP, and PAIN group (n = 28), with subjects who necessarily had MTP. RESULTS: The EMG signal was significantly higher in the muscle portion containing MTP than in the healthy muscle portion in PAIN group and than Healthy group (26,56 ± 44,54, 5,39 ± 6,29 and 1,56 ± 0,76, respectively, p = 0,0001) during rest. In addition, a higher intensity of the EMG signal was observed in subjects with active MTP than in latent MTP (17,85 ± 30,25 versus 3,74 ± 1,52, p = 0,04. Besides, the subjects who presented autonomic phenomena had higher EMG signal than those who had no autonomic phenomena (16,78 ± 28,44 versus 3,51 ± 3,65. CONCLUSION: The surface EMG is reliable to measure the muscular activity of the MTP, mainly at rest condition.
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Humans , Male , Female , Electromyography , Muscle Tonus , Myofascial Pain SyndromesABSTRACT
OBJECTIVE To study the application effectiveness of monitoring-training-planning(MTP) mode in the antibiotics use of gynecological and obstetric surgery.METHODS MTP together with propaganda and education,supervision and management and other strategies were employed to make three rounds circulatory intervention with the antibiotics use for by hysteromyoma and cesarean section operation.By analyzing the days of the antibiotics use,the cost of antibiotics,the rate of combined use and the nosocomial infection(NI) rate of same wards during the same period before and after MTP respectively.RESULTS The days of the antibiotics use,the cost of antibiotics and the rate of triple use have all decreased after each round MTP(P
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OBJECTIVE To study the effect of the intervention of monitoring-training-planning(MTP) on use of antibacterials and injections for pediatric acute upper respiratory infection.METHODS In accordance with selected drug use indicators(SDUIS),the pediatric outpatient prescription of acute upper respiratory infection was selected in our hospital from Jun to Aug 2006 as the baseline,and intervented by MTP,then the investigation of post-interfervention was carried out after a month,and then re-intervention research was made,so the cycle continued.RESULTS The percentage ratio of antibacterials,and injection usage,and the average drug fee in pediatric outpatient prescription for acute upper respiratory infection in our hospital was decreased from 75.00%,61.67%,40.33% and 54.29 yuan to 7.00%,5.00%,3.00% and 39.16 yuan,respectively after four rounds of MTP(all P
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We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.
PCR utilizando vários oligonucleotídeos para detecção de espécies de micobactérias em espécimes clínicas foi padronizado. Três diferentes fragmentos genômicos: da seqüência de inserção IS6110, presente no complexo M. tuberculosis, do gene responsável por proteína específica (32kDa) do gênero e do gene mtp40 espécie-específico do M. tuberculosis foram estudados. A sensibilidade e especificidade do método em 135 amostras clínicas foram de 94.5% e 95.9%, respectivamente, comparados com os resultados da cultura em meio Löwenstein-Jensen, e o limite de detecção foi de 0.05 ng of DNA. O ensaio mostrou-se confiável e rápido para detecção de espécies de Mycobacterium em amostras clínicas, diferenciando M. tuberculosis de M. bovis em uma única reação.
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Objective: To investigate how fatty liver was developed in ventromedial hypothalamus(VMH)-lesioned obese rats. Methods: Two groups of rats were prepared: (1)VMH-lesioned obese rats, and (2)sham VMH-lesioned rats. One week after VMH lesions, livers of all rats were isolated for morphological observation and for determination of microsomal triglyceride transfer protein(MTP), phosphatidate phyosphohydrolase (PAP), malic enzyme (ME), and glucose-6-phosphate dehydrogenase(G6PDH). Results: Triglyceride contents in livers of VMH-lesoned obese rats increased significantly, and were about 1.8-fold of control group. Activities of ME, G6PDH and PAP in the livers were also enhanced markedly compared to their controls. Many lipid droplets in cytoplasm of hepatocytes from VMH-lesioned obese rats were observed, while there was no similar finding in hepatocytes of control rats. MTP activity in livers of VMH-lesioned obese rats was higher than that in livers of sham-operated non-obese rats [0.201?0.013 vs. 0.175?0.014 ?g/(mg protein?h),[WTBX]P0.05). Conclusion: Hepatic triglyceride production and activity of MTP were increased in VMH-lesioned obese rats, but magnitude of the latter did not exceed the former. This resulted in hepatic triglyceride accumulation in spite of increase in transport of triglyceride out of liver by MTP. This may contribute to the development of fatty liver in VMH-lesioned obese rats.
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Objective To investigate the mechanism of orotic acid-induced fatty liver in rats. Method Rats were randomly divided into 2 groups,and fed AIN93 diet with or without 1% orotic acid (OA) for 10d. Serum total cholesterol (TC),triglyceride (TG),high density lipoprotein-cholesterol (HDL-C),hepatic lipids concentrations (TG,TC and phospholipids),hepatic enzymes activities and mRNA levels of key enzymes related to lipids metabolism,as well as hepatic genes expression of transcription factors were determined. Results OA administration significantly increased serum and hepatic TG concentration. The activity and mRNA level of fatty acid synthase (FAS) were obviously up-regulated by OA treatment,whereas the activities and mRNA concentrations of carnitin palmitoyl transferase (CPT) and microsomal triglyceride transfer protein (MTP) were depressed significantly. Furthermore,OA also stimulated the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c),but did not alter the mRNA concentrations of peroxisome proliferator-activated receptor alpha (PPAR?) in liver. Conclusion:The stimulation of TG synthesis caused by enhancement of SREBP-1c and its target genes-FAS,which could be responsible for development of fatty liver. On the other hand,the inhibition of fatty acid beta-oxidation and VLDL secretion were related to the observed lipids accumulation.