Chemical constituents from Mangifera indica seed kernel and their effects on sperm quality of asthenospermia rats in vitro / 中草药

Objective: To study the chemical constituents of mango (Mangifera indica) seed kernel and their effects on sperm quality of asthenospermia rats in vitro. Methods: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, and recrystallization. Their structures were elucidated by spectral analyses such as 1H-NMR, 13C-NMR, and MS. The rats were treated with a single ig administration tripterygium glycosides to induce asthenospermia rat model, so that the effects of the compounds could be evaluated on the sperm motility and sperm survival rate of asthenospermia rats in vitro. Results: Nine compounds were isolated and purified from mango seed kernel, whose structures were elucidated as vitamin E (1), methyl gallate (2), kaempferol (3), quercetin (4), 3,4-O-isopropylidene shikimic acid (5), 5-hydroxy-4-oxopyran-2-carboxylic acid (6), ferulic acid (7), isoquercitrin (8), and 1,2,3,4,6-penta-O-galloylglucose (9). Compounds 1, 7, and 9 could increase the sperm motility and sperm survival rate of asthenospermia rats in vitro. Conclusion: Compounds 1, 3-6 and 8 are obtained from mango seed kernel for the first time; Compounds 1, 7, and 9 could improve the sperm quality of asthenospermia rats in vitro.

Growth of macrobrachium rosenbergii fed with mango seed kernel, banana peel and papaya peel incorporated feeds.

The growth promoting potential of fruits wastes, mango seed kernel, banana peel and papaya peel on the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) was evaluated. Basal diet equated to 35% protein was prepared by using soybean meal, groundnut oilcake, horse gram and wheat flour. Each fruit waste powder was separately incorporated with basal diet at a proportion of 10%. Sunflower oil was used as lipid source. Egg albumin and tapioca flour were used as binding agents. Vitamin B-complex with Vitamin-C was also mixed. Feed without any fruit waste was served as control. M. rosenbergii PL (length: 1.2-1.4 cm; weight: 0.09- 0.13 g) was fed with these feeds for a period of 90 days. Significant improvements in the nutritional indices (survival rate, weight gain, biomass index, specific growth rate and condition factor), concentrations of biochemical constituents (total protein, carbohydrate and lipid), levels of non-enzymatic antioxidants (vitamin-C and E), content of minerals (Na+ and K+), activities of digestive enzymes (protease, amylase and lipase), and profiles of essential amino acids and fatty acids were recorded in fruits wastes incorporated feeds fed PL when compared with control (P< 0.003 – 0.878). The overall results indicated the fact that mango seed kernel incorporated feed was produced the best performance, followed by better performance of banana peel and good performance of papaya peel. These fruits wastes incorporated feeds enhance digestive enzymes activities and act as appetizer, which in turn enhances food utilization and ultimately yielded better survival and growth of M. rosenbergii PL. Therefore, these fruits wastes have considerable potentials in sustainable development of Macrobrachium culture.