Method for determination of the effective dose ED50

There are 4 principle of determination of ED50, the dose effective on 50% of experimental animals. Animals had been distributed into various analogue for diverse doses of studied substance. The evaluation will be carried according to the principle of "yes/no". For 1st example: To determine ED50 concerning the anti-fibrillation effect of venous infective propranolol on BaCl2 included heart fibrillation experimental model of rabbit.Result showed an efficacious dose of ED50 = 1,97mg/kg. For 2nd example: to determine ED50 concerning the hypoglycemic effect of glibenclamid on alloxan included hyperglycemic experimental model on white rat. Result showed on efficacious dose of ED50 = 6,02mg/kg