ABSTRACT
Objective To detect the expression level and correlation of interleukin 35(IL-35) and micro RNA-21(miR-21) in septic patients and to explore the role of their mutual regulation in sepsis .Methods The serum was collected from the patients with sepsis and healthy controls .The expressions of serum IL-35 and miR-21 were detected by ELISA and real-time PCR ,and their correlation was analyzed ;mice were intraperito-neally injected by lipopolysaccharide(LPS) to establish the sepsis model ,then the mice were given anti-IL-35 p35 antibody ,IgG ,sepsis miR-21 mimics(agomir-21) and sepsis miR-21 negative control(agomir-21 NC) by in-traperitoneal injection ,after that ,the serum miR-21 expression level in each group was detected by real-time PCR and IL-35 ,TNF-αand IL-6 levels were detected by ELISA .Results The expression levels of serum IL-35 and miR-21 in sepsis patients were significantly increased compared with the healthy control group ,the difference was statistical significance (P<0 .05) ,moreover their expression levels showed the positive correla-tion (P<0 .05);after septic mice antagonizing to IL-35 ,the serum miR-21 expression was significantly in-creased ,meanwhile the expressions of inflammatory factor TNF-α and IL-6 were increased obviously ,the difference was statistical significance (P<0 .05) .After treating the septic mice by agomir-21 ,the serum IL-35 level was decreased significantly ,while the expression of inflammatory factors TNF-αand IL-6 were increased significantly ,the difference was statistical significance(P<0 .05) .Conclusion Serum IL35 and miR-21 in the patients with sepsis are jointly participate in the septic inflammatory response by mutual regulation .
ABSTRACT
MicroRNA (miRNA) is an endogenous nonprotein-coding short-chain RNA,and it involves in the evolution of a variety of malignant tumors through targeted inhibition of mRNA translation or mRNA cleavage of gene expression regulation cancer or tumor suppressor genes,meanwhile,is also an important molecule in the occurrence and development processes of tumor.In the recent studies,miRNA has played an important role in the proliferation,apoptosis and invasion of cholangiocarcinoma cells,and it maybe become a new biomarker for the early diagnosis,assessment of prognosis and chemotherapy of cholangiocarcinoma.
ABSTRACT
ObjectiveTo investigate the expression of miR-21 in diffuse large B cell lymphoma (DLBCL)and normal lymph tissues and its potential relevance with clinicopathological characteristics.MethodsThe expression levels of miR-21 in 50 primary DLBCL and 12 normal lymph node tissue specimens were examined by TaqMan real-time polymerase chain reaction.The expression of bcl-2 and p53 was detected by immunohistochemistry staining. ResultsThe expression of miR-21 was significantly higher in tumor tissues than that in normal tissues, in GCB subtypes higher than in non-GCB subtypes. And it was negatively correlated with bcl-2(P=0.020),while positively correlated with p53(P=0.022). Up-regulated miR-21 expression was low in three years of survival rate. ConclusionMiR-21 may indicate a more aggressive phenotype and serve as a molecular prognostic marker in DLBCL. High-expression of miR-21 is a key feature that is correlated with cell proliferation in DLBCL.miR-21 may have some guiding significance in prognosis.bcl-2,p53 is possibly one of the targets of miR-21 in DLBCL.