Synthesis and antibacterial activities of phosphonate derivatives containing aminothiazoloxime fragment / 药学学报

Based on the principle of molecular hybridization, fifteen compounds were designed and synthesized through the combination of aminothiazoloxime and phosphonate fragment. The results showed that these compounds had better inhibitory effects on the tested bacteria. In particular, the activities of compounds Ⅲf and Ⅲi against S. aureus, E. coli, methicillin-resistant S. aureus (MRSA) and fluoroquinolone-resistant E. coli (FREC) were the most significant, the minimal inhibitory concentration (MIC) of Ⅲf was 1, 8, 4, 16 μg·mL-1 respectively, and the MIC of Ⅲi was 4, 4, 16, 8 μg·mL-1 respectively, which were slightly lower than that of the control drug oxacillin, and their anti-E. coli, MRSA and FREC activities were superior to that of the control drug oxacillin. Their activities to S. aureus were close to that of oxacillin, and to E. coli, MRSA and FREC were superior to that of oxacillin, which is worthy of further study.

Synthesis and antileishmanial activity of naphthoquinone-based hybrids / Síntesis y actividad antileishmania de híbridos naftoquinónicos / Síntese e atividade antileishmania de híbridos naftoquinónicos

SUMMARY Introduction: Leishmaniasis is a disease caused by protozoa of the genus Leishmania and is considered endemic in 98 countries. Treatment with pentavalent antimonials has a high toxicity, which motivates the search for effective and less toxic drugs. α- and β-lapachones have shown different biological activities, including antiprotozoa. In recent studies, the isonicotinoylhydrazone and phthalazinylhydrazone groups were considered innovative in the development of antileishmania drugs. Molecular hybridization is a strategy for the rational development of new prototypes, where the main compound is produced through the appropriate binding of pharmacophoric subunits. Aims: To synthesize four hybrids of α- and β-lapachones, together with the isonicotinoylhydrazone and phthalazinylhydrazone groups and to determine the antileishmania activity against the promastigotic forms of L. amazonensis, L. infantum and L. major. Results: β-lapachone derivatives were more active against all tested leishmania species. βACIL (IC50 0.044μM) and βHDZ (IC50 0.023μM) showed 15-fold higher activity than amphotericin B. The high selectivity index exhibited by the compounds indicates greater safety for vertebrate host cells. Conclusion: The results of this work show that the hybrids βACIL and (3HDZ are promising molecules for the development of new antileishmania drugs.

RESUMEN Introducción: Leishmaniasis es una enfermedad causada por protozoos del género Leishmania y se considera endémica en 98 países. El tratamiento con antimoniales pentavalentes tiene una alta toxicidad, lo que motiva la búsqueda de fármacos eficaces y menos tóxico. α- y β-lapachones han mostrado diferentes actividades biológicas, incluido los antiprotozoarios. En estudios recientes, los grupos isonicotinoilhidra-zona y ftalazinilhidrazona se consideraron innovadores en el desarrollo de fármacos antileishmania. La hibridación molecular es una estrategia para el desarrollo racional de nuevos prototipos, donde el compuesto principal se produce a través de la unión apropiada de subunidades farmacofóricas. Objetivos: Sintetizar cuatro híbridos de α- y β-lapachones, junto con los grupos isonicotinoilhidrazona y ftalazinilhidrazona y determinar la actividad antileishmania frente a las formas promastigotas de L. amazonensis, L. infantum y L. major. Resultados: Los derivados de β-lapachone fueron más activos contra todas las especies de leishmania probadas. La βACIL (CI50 0,044μM) y βHDZ (CI50 0,023μM) mostraron actividad 15 veces mayor que la anfotericina B. El alto índice de selectividad que presentan los compuestos indica una mayor seguridad para las células huésped del vertebrado. Conclusión: Los resultados de este trabajo demuestran que los híbridos (ACIL y (HDZ son moléculas prometodoras para el desarrollo de nuevos fármacos antileishmania.

RESUMO Introdução: A leishmaniose é uma doença causada por protozoários do género Leishmania e é considerada endémica em 98 países. O tratamento com antimoniais pentavalentes apresenta alta toxicidade, o que motiva a pesquisa por medicamentos eficazes e menos tóxicos. α- e β-lapachones tém mostrado diferentes atividades biológicas, incluindo antiprotozoários. Em estudos recentes, os grupos isonicotinoilhi-drazona e ftalazinilhidrazona foram considerados inovadores no desenvolvimento de drogas antileishmania. A hibridização molecular é uma estratégia para o desenvolvimento racional de novos protótipos, onde o composto principal é produzido através da ligação apropriada de subunidades farmacofóricas. Objetivos: Sintetizar quatro híbridos de α- e β-lapachones, juntamente com os grupos isonicotinoil-hidra-zona e ftalazinilhidrazona e determinar a atividade antileishmania contra as formas promastigóticas de L. amazonensis, L. infantum e L. major. Resultados: Os derivados de β-lapachona foram mais ativos contra todas as espécies de leishmania testadas. BACIL (IC50 0,044 μM) e βHDZ (IC50 0,023 μM) apresentaram atividade 15 vezes maior do que a anfotericina B. O alto índice de seletividade dos compostos indica maior segurança para células hospedeiras de vertebrados. Conclusaõ: Os resultados deste trabalho mostram que os híbridos βACIL e βHDZ são moléculas promissoras para o desenvolvimento de novos fármacos antileishmania.

Hydrazones derived from natural aldehydes: in vitro cytotoxic evaluation and in silico pharmacokinetic predictions / Hidrazonas derivadas de aldehídos naturales: evaluación citotóxica in vitro y determinación del perfil farmacocinético in silico / Hidrazonas derivadas de aldeídos naturais: avaliação citotóxica in vitro e determinação de perfil farmacocinético in silico

SUMMARY Introduction: Recent research has reported the cytotoxic potential of hydrazones against various strains of cancer cells. Aim: To evaluate the anticancer activity in vitro and the pharmacokinetic profile of six synthesized hydrazonic compounds, identified as vanillin 1-phthalazinylhydrazone (VAN-1); 2,4-dinitrophenylhydra-zone vanillin (VAN-2); phenylhydrazone cinnamaldehyde (CIN-1); isonicotinoyl hydrazone cinnamaldehyde (CIN-2); cinnamaldehyde 1-phthalazinylhydrazone (CIN-3); and 2,4-dinitrophenylhydrazone cinnamaldehyde (CIN-4). The cytotoxic activity was evaluated against four strains of cancer cells. Methodology: The pharmacokinetic parameters of absorption, distribution, metabolism, excretion, and toxicity (ADME/T) of the hydrazones were evaluated using the PreADMET program. Results: Hydrazones derived from cinnamaldehyde (CIN-1 and CIN-2) showed high cytotoxic activity against leukemic (HL-60) and glioblastomas (SF-295) cell lines. The pharmacokinetic profile of the hydrazones showed that, in general, the hydrazones presented satisfactory characteristics of ADME/T. In addition, it was observed that CIN-2 presented the most promising in silico profile, showing high intestinal absorption, desirable distribution profile related to plasma protein binding, adequate renal excretion, and low toxicity. The ADME/T profile of the CIN-1 compound highlighted its potential as a promising antineoplastic agent with action of the CNS, more specifically against glioblastomas.

RESUMEN Introducción: Investigaciones recientes han informado del potencial citotóxico de las hidrazonas contra varias líneas de células cancerosas. Objetivo: Evaluar la actividad anticancerígena in vitro y el perfil farmacocinético de seis compuestos hidrazónicos sintetizados, identificados como vainillina 1-ftalazinilhidrazona (VAN-1); vainillina 2,4-dinitrofenilhidrazona (VAN-2); fenilhidrazona cinamal-dehído (CIN-1); cinamaldehído de isonicotinoil hidrazona (CIN-2); cinamalde-hído 1-ftalazinilhidrazona (CIN-3); y 2,4-dinitrofenilhidrazona cinamaldehído (CIN-4). Se evaluó la actividad citotóxica frente a cuatro líneas celulares cancerosas. Metodología: Los parámetros farmacocinéticos de absorción, distribución, metabolismo, excreción y toxicidad (ADME/T) de las hidrazonas se evaluaron mediante el programa PreADMET. Resultados: Las hidrazonas derivadas del cinamaldehído (CIN-1 y CIN-2) mostraron una alta actividad citotóxica contra las líneas celulares leucémicas (HL-60) y glioblastomas (SF-295). El perfil farmacocinético de las hidrazonas mostró que, en general, las hidrazonas mostraban características satisfactorias de ADME/T. Además, se observó que CIN-2 presentó el perfil in silico más prometedor, presentando alta absorción intestinal, perfil de distribución deseable relacionado con la unión a proteínas plasmáticas, excreción renal adecuada y baja toxicidad. El perfil ADME/T del compuesto CIN-1 destacó su potencial como agente antineoplásico prometedor con acción sobre el SNC, más específicamente contra los glioblastomas.

RESUMO Introdução: Pesquisas recentes relataram o potencial citotóxico das hidrazonas contra várias linhagens de células cancerígenas. Objetivo: Validara atividade anti-câncer in vitro e o perfil farmacocinético de seis compostos hidrazônicos sintetizados, identificados como vanilina 1-ftalazinil-hidrazona (VAN-1); vanilina 2,4-dinitrofenil-hidrazona (VAN-2); cinnamaldeído de fenil-hidrazona (CIN-1); cinamaldeído isonicotinoil-hidrazona (CIN-2); 1-ftalazinil-hidrazona de cinnamaldeído (CIN-3); e cinamaldeído de 2,4-dinitrofenil-hidrazona (CIN-4). A atividade citotóxica foi avaliada contra quatro linhagens de células cancerígenas. Metodologia: Os parâmetros farmacocinéticos de absorção, distribuição, metabolismo, excreção e toxicidade (ADME/T) das hidrazonas foram avaliados utilizando o programa PreADMET. Resulados: As hidrazonas derivadas do cinnamaldeído (CIN-1 e CIN-2) apresentaram alta atividade citotóxica contra as linhagens celulares leucêmicas (HL-60) e de glioblastomas (SF-295). O perfil farmacocinético das hidrazonas mostrou que, em geral, as hidrazonas apresentaram características satisfatórias de ADME/T. Além disso, observou-se que a CIN-2 apresentou o perfil in silico mais promissor, exibindo alta absorção intestinal, perfil de distribuição desejável relacionado à ligação às proteínas plasmáticas, excreção renal adequada e baixa toxicidade. O perfil ADME/T do composto CIN-1 destacou seu potencial como um agente antineoplásico promissor com ação do SNC, mais especificamente contra glioblastomas.

Application of PCR reverse dot blot in non-syndromic deafness gene detection / 临床耳鼻咽喉头颈外科杂志

Objective@#To detect 20 common deafness gene mutations in non- syndromic deafness patients in China using PCR- RDB, and analyze and summarize the mutation data to explore the clinical value of this method. @*Method@#The PCR- RDB and Sanger sequencing were used to detect 20 common mutations of four deafness genes（GJB2, GJB3, SLC26A4 and mtDNA） in 500 patients with non- syndromic hearing loss . The Sanger sequencing was used to compare the sensitivity, specificity, positive predictive value, negative predictive value, and total coincidence rate of the deafness mutation detected by PCR- RDB. @*Result@#A total of 500 samples were detected. 147 wild- type samples, 81 homozygous mutant samples, 240 heterozygous mutant samples, 32 composite heterozygous mutant samples were detected using the PCR- RDB within the range of 20 gene mutations, which were identical to the Sanger sequencing results. GJB2 c.235delC and SLC26A4 c.919- 2 A>G are the most common hotspot mutations in this study, followed by mtDNA m. 1555 A>G. Compared with the Sanger sequencing method, the sensitivity, specificity, positive predictive value, negative predictive value, and total coincidence rate of the real- time fluorescence PCR melting curve method were 100%, and the Kappa value was one. @*Conclusion@#PCR reverse dot-blot hybridization is a simple, rapid, sensitive and specific method for detecting 20 mutations of 4 common deafness genes in Chinese population, it is expected to be used in clinical detection of deafness genes in the future.

Application of PCR reverse dot blot in non-syndromic deafness gene detection / 临床耳鼻咽喉头颈外科杂志

To detect 20 common deafness gene mutations in non- syndromic deafness patients in China using PCR- RDB, and analyze and summarize the mutation data to explore the clinical value of this method. The PCR- RDB and Sanger sequencing were used to detect 20 common mutations of four deafness genes（, and ） in 500 patients with non- syndromic hearing loss . The Sanger sequencing was used to compare the sensitivity, specificity, positive predictive value, negative predictive value, and total coincidence rate of the deafness mutation detected by PCR- RDB. A total of 500 samples were detected. 147 wild- type samples, 81 homozygous mutant samples, 240 heterozygous mutant samples, 32 composite heterozygous mutant samples were detected using the PCR- RDB within the range of 20 gene mutations, which were identical to the Sanger sequencing results. GJB2 c.235delC and SLC26A4 c.919- 2 A>G are the most common hotspot mutations in this study, followed by mtDNA m. 1555 A>G. Compared with the Sanger sequencing method, the sensitivity, specificity, positive predictive value, negative predictive value, and total coincidence rate of the real- time fluorescence PCR melting curve method were 100%, and the Kappa value was one. PCR reverse dot-blot hybridization is a simple, rapid, sensitive and specific method for detecting 20 mutations of 4 common deafness genes in Chinese population, it is expected to be used in clinical detection of deafness genes in the future.

Uso de técnicas moleculares na detecção de DNA parasitário e no estudo da variabilidade genética em diferentes fragmentos teciduais de cães naturalmente infectados por Leishmania (Viannia) braziliensis / Use of molecular techniques in parasite DNA detection and genetic variability study in different tissue fragments from naturally infected dogs by Leishmania (Viannia) braziliensis

A leishmaniose tegumentar americana (LTA), em cães, costuma estar associada à resposta humoral baixa ou mesmo negativa, o que inviabiliza os métodos sorológicos convencionais, como ferramenta única no diagnóstico. [...] Métodos convencionais de diagnóstico parasitológico, não têm sido capazes de detectar a presença do parasito em outros sítios anatômicos diferentes da lesão cutânea, em cães naturalmente infectados por Leishmania (Viannia) braziliensis. Diante dos questionamentos sobre o papel do cão doméstico no ciclo de transmissão da LTA e sobre o valor de métodos diagnósticos aplicados na rotina, principalmente em áreas de sobreposição da forma tegumentar e visceral, faz-se necessário a avaliação desses animais sob diversos aspectos. O presente estudo teve como objetivo empregar a PCR específica associada à hibridização molecular e a técnica da reação em cadeia da polimerase com primer único em condições de baixa estringência (LSSP-PCR) visando detectar a presença de DNA parasitário e investigar a variabilidade genética de populações parasitárias presentes em diferentes tecidos de cães naturalmente infectados por L. (V.) braziliensis. Os animais foram selecionados entre os cães sororeatores para Leishmania procedentes de cidades do estado do Rio de Janeiro e encaminhados para eutanásia ao Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos do Instituto de Pesquisa Clínica Evandro Chagas da Fundação Oswaldo Cruz. Durante a necropsia, foram obtidas amostras de lesão cutânea, pele íntegra (região escapular e abdominal), baço, fígado e linfonodos (poplíteo e cervical)...

In dogs, American tegumentary leishmaniasis (ATL) is usually associated to a low humoral responseor even to negative results which turns not unfeasible the conventional serological methods. [...] Conventional methods ofparasitological diagnosis have failed to detect the presence of the parasite anatomic sites others thanthe cutaneous lesion, in dogs naturally infected by Leishmania (Viannia) braziliensis. Regarding thequestions on the rule of domestic dogs in the transmission cycle of ATL and, on the applicability ofdiagnostic methods mainly in areas where both visceral and tegumentary disease are found, theevaluation of these animals under different aspects is needed. The objective of this project is to applyspecific PCR assays associated to molecular hybridization and the technique of Low-StringencySpecific-Single Primer – PCR (LSSP-PCR) in order to detect the presence of parasite DNA andevaluate the genetic variability of parasite populations found in different tissues of dogs naturallyinfected by Leishmania (V.) braziliensis...

Detection of hepatitis B virus genotype and the relationship between hepatitis B virus genotype and the function of liver / 西安交通大学学报(医学版)

Objective To investigate the relationship between hepatitis B virus (HBV) genotype and liver function. Methods The method of microboard nucleate molecular hybridization was employed to detect the genotype in 93 HBV patients of different clinical types and the function of liver. Results Among the 93 HBV patients of different clinical types, there were 24 cases of genotype B (25.81%), 59 cases of genotype C (63.44%), 5 cases of genotype D (5.38%), and 5 cases of mixed type (3 cases of B/D, 2 cases of C/D, 5.38%). Therefore, genotype C took up the largest proportion, followed by genotype B, and then D and mixed genotypes, but there was no genotype A, E or F. The detection rate of genotype C increased according to the sequence of chronic hepatitis B, subacute severe hepatitis and hepatocirrhosis while the detection rate of genotype B decreased gradually. However, the detection rate of genotype C in hepatocellar carcinoma did not rise correspondingly. The levels of ALT, AST and TBIL of genotype C were higher than those of genotype B, but the level of ALB in genotype C was lower than that of genotype B. None of the differences had significance. Conclusion Most of HBV genotypes in Xi'an were C, some of them were B, D and mixed genotypes, but no genotype A, E or F was detected. Except hepatocellar carcinoma, the detection rate of genotype C rose according to the severity of clinical type.

The detection of hepatitis B virus genotype and its clinical dependability / 西安交通大学学报(医学版)

0.05). The positive rate of HBeAg in genotype C(69.7%) was significantly higher than that in genotype B(42%) (P

Observation on the Expression of HBV-DNA in the Umbilical Cord Tissuesof the Died Fetus Delivered From Puerpera with Hepatitis Virus B / 中国医师杂志

Objective To observe the expression of HBV-DNA and whether there were copy of HBV in the umbilical cord tissues of the died fetus delivered from puerpera with hepatitis virus B. Methods 40 cases such died fetus were collected by routine autopsy to obtain umbilical cord tissues.And using in situ molecular hybridization technique detected HBV-DNA. Results For the umbilical cord tissues, there were 40%(16/40) cases detected out HBV-DNA.HBV-DNA mainly localization in the surface of the umbilical cord vessel and in the cytoplasma of the cord vessel's endothelial cells. They were not in the cord vessel's endothelial cells nuclei. Conclusions There were HBV replication in the umbilical cord tissues of the died fetus. But the expression of HBV-DNA in the umbilical cord tissues of the died fetus is not related with the HBV replication status in the pregnant woman veins.

Expression of HBV-DNA in the Umbilical Cord Tissues of The Dead Fetus Delivered From Puerperant With Hepatitis Virus B Infection / 中国医师杂志

0 05). HBV-DNA were mainly localized on the surface of the umbilical cord vessel and in the plasma of the cord vessel's endothelial cells. HBV-DNA in the cord vessel's endothelial cells nuclei was not seen.Conclusion There were HBV replication in the umbilical cord tissues of the died fetus. But expression of HBV-DNA in the umbilical cord tissues of the died fetus was not related with the HBV replication status in the pregnant woman veins.

Hepatitis B virus (HBV) infections in turtles

Thirty turtles (15 Clemys mutica and 15 Geoclemys reevesii) which were inoculated with human sera those were positive for hepatitis B surface antigen (HBsAg) and hepatitis B "e" antigen (HBeAg) were found to be infected with hepatitis B virus (HBV). The levels of HBV infection markers, such as HBsAg and antibody to HBsAg (anti-HBsAg), were retinely monitored in the turtles' serum for 46 weeks. Within two weeks of the inoculation, 42% of the turtles tested were positive for HBsAg, and their reciprocal titers as measured by reverse passive hemagglutination (RPHA) and enzyme linked immunoabsorbance assay (ELISA) ranged from 16 to 96. Within 20 weeks, the remaining turtles tested HBsAg positive, as confirmed by ELISA. At 20 weeks, all but one of the turtles exhibited changes in HBV blood marker from HBsAg to anti-HBs; the one exception was positive for both HBsAg and anti-HBs. At the 47th week, 7 animals were killed and their organs were examined for HBV infected cells utilizing an immunofluorescent technique. Numerous fluorescent cells which reacted with human anti-HBs nad anti-HBc were observed in the following organs: pancreas, liver, kidney, and brain. Histopathologically, edematous changes in hepatocytes and minor cellular infiltration attributed to an inflammatory response were noted. Liver and kidney cells from the infected animals were cultured, and HBV antigen positive cells for HBsAg and HBcAg were detected in the cultures. Throughout the experiment, HBsAg was detected in the supernatant by ELISA. Virus particles which were indistinguishable from Dane particles were seen in the cytoplasmic vacuoles of the cultured cells by electron microscopy. Finally, the presence of HBV DNA was established by molecular hybridization techniques in the culture supernatants of kidney cells from the infected turtles.

Investigation of HBV-DNA in peripheral blood mononuclear cells and hepatocytes with molecular hybridization / 第三军医大学学报

The existence of HBV-DNA in the mononuclear cells of peripheral blood and in the serum of 61 patients with HBV infection was determined with southern blot and dot blot hybridization,and that in the liver tissue of 31 patients out of the 61 with southern blot and in situ hybridization.The positive rate of HBV-DNA in the serum,mononuclear cells and hepatocytrs was 26.2% (16/61),24.6% (15/61) and 44.8% (13/31) respectively.There was no concordance of the existence of HBV-DNA in the serum,peripheral mono-nuclear cells and hepatocytes in an individual.For example,HBV-DNA was absent in the serum but present in mononuclear cells and hepatocytes in 11 cases.In fact,peripheral mononuclsar cells can serve as the reservoir for HBV to replicate.It cannot be denied that HBV can replicate in an individual even though HBV-DNA is negative in the serum.

The Induction of hsp70 mRNA under Heat Stress / 第二军医大学学报

The induction of hsp70 mRNA in human osteosarcoma cells (HOS-8603) and intacl rats under heat stress was studied using hsp70 cDNA labeled with a-32P dATP. The results showed that the induction of hsp70 mRNA was evident in liver, lung, spleen and the most evident was found in brain when the core body temperature of rats was brought to 42℃ for 15 min. The induction of hsp70 mRNA in HOS-8603 was also significant when cells were cultured at 42℃ for 30 min. These results indicated that hsp70 mRNA could be induced both in vitro and in vivo under heat stress condition and the induction of hsp70 mRNA in intact rats was of tissue-specific fashion.

Down-regulation of Gucocorticoid Receptor mRNA by Glucocorti-coids in Rats / 第二军医大学学报

The effect of glucocorticoids on the down-regulation of glucocorticoid receptor (GR) mRNA was studied in intact rats. GR mRNA was characterized by Northern blot hybridization and quantitated by dot blot hybridization using a human GR cDNA fragment as a probe. Administration of hydrocortisone in polyvinyl alcohol resulted in a rapid increase in plasma glucocorticoids which maintained at stress levels (20 to 40 ?g/dl) for about 3 d. Hepatic GR mRNA decreased significantly to 73.5?63% of control values 6 h following hydrocortisone treatment, after which the decline of GR mRNA was gradual, reaching a minimum of 44.0?5.0% of control levels 3 d after the treatment The effect of hydrocortisone on the down-regulation of hepatic GR mRNA lasted up to 11 d. In contrast, hydrocortisone treatment had no effect on GR mRNA in rat brain. These results are consistent with the changes in GR in rats as reported previously, except that even though the hepatic cytosol GR decreased markedly, no significant changes in hepatic GR mRNA were found 1 h after hydrocortisone treatment, strongly suggesting that the down-regulation of GR by its ligands in vivo occurs at both transcriptional and posttranscriptional levels and is of tissue-specific fashion.

Application of Dialysis-type Molecular Species Hybridizable Method in the Genetic Monitoring Studies / 第二军医大学学报

By means of the dialysis-type method of molecular species hybridization the individual differ ences of polypeptide chains of globin of SMMC/B (abbreviated as B) mice at the filial generations of F28 and F34, and at the same parental generation Bp (?) and Bp ( + ) as well as F35 (Bf) were investigated. The results of hybridization between mouse Hb and sheep Hb are as follows: All the hybrid groups exhibited four zones on polyacrylamide gel electrophoretogram, but all the control groups only two zones. The comparison of electrophoretograms showed that the electrophoretic positions of the new components were the same, indicating ? and ? chains assayed were quite close to each other, and no significant differences among individual and intergenerations were observed in B mice globins. By electrophoretogram analysis of the genetic expression products, we might distinguish preliminarily the genetic variation of ?- and ?-genes.

IDENTIFICATION OF HUMAN PAPILLOMAVIRUSES DNA IN LESIONS FROM WOMEN WITH CONDYLOMA ACUMINATA BY MOLECULAR HYBRIDIZATION / 西安交通大学学报(医学版)

HPV DNA were detected in 60 biopsies from women with condyloma acuminata (vulva 47,Vagina 12,cervix 1)by dot blot hybridiztion;physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization. HPV 11 was found in 19biopsies of vulva (13 ). vagina (5)and cervix (1),and HPV 16 and 18 in lvaginal and 1 vulvar biopsy respectively. All of HPV 11 DNA were episomal in cells . Our results provided the data for further studying the relationship between HPV and genital cancer in our country.

STAGE-DEPENDENT EXPRESSION OF ANDROGEN RECEPTOR AND FSH RECEPTOR IN ADULT RAT TESTIS / 解剖学报

Objective To determine the cellular localization and expression pattern of AR and FSHR in adult rat testis must be helpful to understand the action site and mechanisms that T and FSH regulate spermatogenesis. Methods We applied in situ Hybridization to detect the expression of AR and FSHR on adult testis, in which Dig labeled cRNA probe was used to carry out the experiment on frozen sections; at the same time, following the technique of transillumination assisted microdissection we separated seminiferous epithelium into four stages(Ⅱ Ⅵ,Ⅶ Ⅷ,Ⅸ Ⅻ and ⅩⅢ Ⅰ), extracted total RNA and carried out dot hybridization, using ? 32 P labeled cDNA probe, in order to test qualitatively and quantitatively the location of AR and FSHR mRNA and their expression pattern in adult rat testis. Results Our results showed that the positive signal of AR mRNA was located in Sertoli cells and Leydig cells. The signal in Sertoli cells began to appear in Ⅱ Ⅵ stages, strongest in Ⅶ Ⅷ stages and weakest in Ⅸ Ⅰ stages ( P