ABSTRACT
The spectrophotometric multi-component analysis involves spectrum recording and mathematical equations. However, spectral interference poses a major limitation when mixture samples are encountered. To overcome this derivative spectrophotometry (DS) has been introduced for the resolution of overlapping peaks. In this review modified methods like derivative quotient spectra, double divisor ratio spectra derivative method, double divisor means centering of ratio spectra method, derivative subtraction coupled with the constant multiplication method (DS-CM), amplitude subtraction (AS), modified amplitude subtraction (MAS), amplitude factor method (P-Factor), amplitude modulation method (AM), amplitude summation method (A-Sum), simultaneous derivative ratio spectrophotometry (S1DD), derivative compensation ratio via regression equation, differential dual wavelength (D1 DWL), differential derivative ratio (D1DR), successive derivative subtraction method (SDS) and derivative transformation (DT) of derivative spectrophotometry theories and applications are reviewed. These methods were applied to solve different complex pharmaceuticals mixtures. These developed methods were simple and cost-effective.
ABSTRACT
Objective: To study the dynamic processes of in vivo metabolism of multicomponent in the decoction of Angelicae Sinensis Radix (ASR), select the quality control components, look for a new quality evaluation method for Chinese materia medica (CMM). Methods: The blood was taken from mesenteric vein and abdominal aorta. By analyzing HPLC fingerprints of intestinal metabolism and liver metabolism, we could infer multicomponent changes in the metabolic process of water extract from ASR. Results: Totally 15 components were basically stable in the digestive juice, four of them may be metabolized by intestine, while seven of them were metabolized by liver. And we found one new component was the metabolite of liver, seven new components were the metabolites of intestine, but five of them were metabolized by liver and could not be absorbed into blood. Finally, we identified that four prototype components of water extract from ASR and three metabolites could be absorbed into blood. Conclusion: Based on the multicomponent metabolic profiling of ASR, we initially select the four prototype components including senkyunolide I as the quality control component, and we finally establish a quality evaluation method for "correlation in quality and metabolism".