ABSTRACT
To investigate the effect of Fuke Qianjin tablets on the pharmacokinetics of Azithromycin(AZM) in rats with chronic pelvic inflammation, and evaluate the rationality of the drug combination. The animal models of chronic pelvic inflammatory disease were established by intrauterine injection of mixed bacterial suspension plus mechanic injury in female Sprague-Dawley(SD) rats. The model rats were randomly divided into control group and drug combination group. The rats in control group were given with Azithromycin 10 mg•kg⁻¹•d⁻¹ by intragastric administration, while the rats in drug combination group were given with Fuke Qianjin tablets 1.66 g•kg⁻¹•d⁻¹ by intragastric administration 2 hours after the equivalent dose of azithromycin, once a day, consecutively for 7 days. Plasma samples were collected at different time points and the concentration of AZM in plasma was determined by high performance liquid chromatography coupled with mass spectrometry(HPLC-MS). ADAPT 5.1 software was used to calculate the pharmacokinetic parameters of each group by Inverse Gaussian Function model. SPSS software was used for the statistical analysis of data in each group. The results showed that Fuke Qianjin tablets had no significant effects on the main pharmacokinetic parameters of AZM, including CLt, CLd, MIT and F. The study showed that Fuke Qianjin tablets can be combined with azithromycin for treatment of chronic pelvic inflammation disease.
ABSTRACT
Objective: To carry out the pharmacokinetic study in awake animal after multiple dosing of Danshen (Salvia miltiorrhiza) Decoction by blood microdialysis method combined with LC-MS. Methods: The pharmacokinetic study was carried out with awake rabbits as experimental animal, Danshen Decoction as tool drug, and Danshensu as evaluation index combining with microdialysis sampling and LC-MS detection technology after intraday multiple dosing. The pharmacokinetic parameters were obtained by non-compartmental model method. Results: The pharmacokinetic study pattern of multiple dosing was established in awake animal, and the whole process dynamic characteristics were collected. The method was stable, reliable, and simple. Conclusion: This study can make the reference to similar study for other drugs.