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Abstract Background: Panic disorder has long been associated with the changes in various neurotransmitters, such as Neuropeptide-S (NPS). Objective: In this study we aimed to determine whether there is a relationship between blood NPS levels and panic disorder. Methods: Twenty nine patients with panic disorder and thirty two healthy control subjects who were age and gender matched were enrolled to the study. Blood samples were taken from participants and plasma NPS levels were quantified by using an ELISA kit. Results: In the study group, median NPS blood level was 16.7 pg/mL and in the control group it was 32.5 pg/mL. There was a statistically significant difference (p = 0.021). Using receiver operating characteristics (ROC) curve, sensitivity and specificity of NPS blood level, for diagnosing panic disorder was calculated, and it was found 79.3% and 56.25% respectively (AUC:0.672, 95% CI: 0.540-0.787). Discussion: Malfunction at the NPS modulatory system in the cortical areas (which is causing excitations in brain areas, such as amygdala and hypothalamus) does not only increase anxiety symptoms and risk of panic disorder but also causes panic disorder patients to have lower plasma NPS levels than the control group. Therefore it can be argued that such malfunction can be treated with a systemic treatment. Baykan H et al. / Arch Clin Psychiatry. 2018;45(4):79-81
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Objective To explore the mediating effects of neuropeptide S receptor 1 and negative coping in chronic stress induced emotional responses. Methods A survey of 366 local residents who were chosen from County of Nandan in Guangxi Province by stratified random sampling method. Questionnaires of the life events scale (LES),the simplified symptom checklist and the Simplified Coping Style Questionnaire (SCSQ) were used. The level of NPSR1 was measured by Elisa. Data was analyzed by SPSS 21 and AMOS 21 statistical software for statistical analysis. Results The level of chronic psychological stress was (25. 24± 29. 26) and score of negative coping was(14. 84±4. 35). Somatic factor,anxiety factor,depressive factor and hostile factor were (1. 58±0. 58),(1. 43±0. 56),(1. 44±0. 55),(1. 23±0. 37). The total score of life e-vents was positively correlated with negative coping,somatic factor score,anxiety factor score,depression fac-tor score(r=0. 23-0. 46,P<0. 05),while negatively correlated with the expression level of detection of NPSR1 in plasma(r=-0. 26,P<0. 05). NPSR1 level was negatively correlated with body factor score,anxiety factor score,depression factor score and hostility factor score(r=-0. 18--0. 61,P<0. 05). Negative coping was positively correlated with body factor score,anxiety factor score,depression factor score and hostility fac-tor score(r=0. 12- 0. 27,P<0. 05). Both neuropeptide S receptor 1 and negative response had significant me- diating effects on emotional responses induced by chronic psychological stress (P<0. 05),and the effect val-ues were -0. 08 and 0. 05,respectively. According to the Bootstrapping method,the results of the mediation model showed that Bias-Corrected of 95% CI of the total standardization effect,the indirect standardization effect,the direct standardization effect were not including 0. Conclusion NPSR1 and negative coping plays a dual mediating effect on stress induced emotional responses.
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Background:Studies showed that neuropeptide S receptor 1(NPSR1)gene polymorphisms were associated with the susceptibility of inflammatory bowel disease( IBD)in Europe’s populations,however,there is no study on the relevance in Chinese population. Aims:To investigate the association of NPSR1 gene polymorphisms with IBD in Chinese Han population. Methods:A total of 457 IBD patients[355 cases of ulcerative colitis(UC)and 102 cases of Crohn’s disease ( CD)]from Zhongnan Hospital of Wuhan University and 500 healthy controls were recruited. Genotyping of 2 single nucleotide polymorphisms(SNPs)of NPSR1,rs323922(C→G mutation)and rs740347(G→C mutation)was performed by using PCR and sequencing techniques. Results:Differences of the frequencies of genotypes and alleles for rs323922 and rs740347 between UC,CD patients and controls didn’t reach statistical significance(P >0. 05). Genotype-phenotype analysis showed that there were some impact of genotypes for rs323922 and rs740347 on clinical phenotypes of IBD:( i) For rs323922,mutant CG genotype was correlated with male CD patients(OR:0. 441,95% CI:0. 230-0. 844)and CD involving the colon(OR:0. 425,95% CI:0. 199-0. 911),and might be a protective factor.(ii)For rs740347,mutant CC genotype was correlated with early onset CD patients( <16 years old)(OR:15. 019,95% CI:2. 634-86. 470)and mutant C allele was correlated with CD involving the colon(OR:2. 142,95% CI:1. 709-4. 294),both were risk factors. Conclusions:Polymorphisms of NPSR1 rs323922 and rs740347 are not contributors of IBD susceptibility in Chinese Han population,but might be correlated with some clinical phenotypes of IBD.
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Objective To observe the changes of the neuropeptide S (NPS) expression in dorsomedial nucleus of rat hypothalamus after rapid eye movement (REM) sleep deprivation. Methods Totally 24 adult male rats were evenly randomized into 3 groups: normal control group (CC), tank control group (TC) and REM sleep deprivation group (SD). Modified multiple platform method (MMPM) was used to establish REM sleep deprivation model in rats. Immunohistochemistry and hybridization in situ were applied to examine the expression of NPS protein and mRNA in the rat hypothalamus. Results There was no significant differences in the expression of NPS protein or mRNA in rats hypothalamus between CC group and TC group. After REM sleep deprivation, NPS protein and mRNA positive cells in rats hypothalamus in SD group were (27. 86 ± 2. 47) and (25. 75±2. 12), respectively, which were significantly more than those in CC group and TC group (16. 75 ± 2. 12 and 19. 63 ± 1. 85 in CC group, 18. 60±1.60 and 18. 50±1.69 in TC group; P
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@#Objective To investigate the effects of intracerebroventricular injection of neuropeptide S (NPS) on pain and anxiety behaviors in rats with neuropathic pain, and explore the possible mechanism. Methods 40 male Sprague-Dawley rats were randomly divided into Sham-Vehicle group, Sham-NPS group, chronic constriction injury (CCI) group, Neuropeptide S low-dose group (NPSl group, 0.1 nmol/L) and Neuropeptide S high-dose group (NPSh group, 1 nmol/L), with 8 rats in each group. Pain-related behaviors, anxiety-related behaviours,and expression of NPS receptor (NPSR) in the left amygdaloid nucleus were measured on the 14th day via intracerebroventricular injection.Results Compared with the Sham-Vehicle group, the CCI group demonstrated significant pain and anxiety behaviors 14 days after operation (P<0.01), the NPSh group significantly relieved (P<0.01). And there was no significant difference between the NPSl group and the CCI group (P>0.05). Compared with the CCI group, NPSR expression in amygdaloid nucleus increased in the NPSh group. Conclusion NPS can dose-dependently relieve the pain and anxiety behaviors in CCI rats, which may be related with the increase of NPSR in amygdaloid nucleus.