ABSTRACT
To establish the fingerprint of Alpiniae Oxyphyllae Fructus by UPLC. The chromatographic fingerprint was obtained with ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) and gradient eluted with acetonitrile and aqueous formic acid. The flow rate was 0.5 mL/min, the sample room temperature and the column temperature were maintained at 10℃ and 30℃, respectively. The detection wavelength was set at 254 nm. The injection volume was 2 μ L. The common mode of UPLC fingerprint of Alpiniae Oxyphyllae Fructus was set up firstly. There were 20 common peaks in the fingerprints. Protocatechuic acid, α-hydroxymethyl furfural, oxyphyllenodiol A, teuhetenone A, teuhetenone A, chrysin, kaempferide, tectochrysin, and nootkatone were identified by comparing the retention time and their ultraviolet spectra. The similarities of 15 Alpiniae Oxyphyllae Fructus among 19 batches of Alpiniae Oxyphyllae Fructus were above 0.970. The method is rapid and efficiency. It can be used for the quality evaluation of Alpiniae Oxyphyllae Fructus.
ABSTRACT
OBJECTIVE: To establish a UPLC method for simultaneous determination of 5-hydroxymethyl furfural (1), leu-hetenone A (2), lectochrysin (3), and nootkatone (4) in Alpinia, oxyphylla Miq, and to compare the contents of the four components in different parts of this medicinal materials from different places. METHODS: The UPLC method was established on an HSS T3 Column (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of water containing 0.1% formic acid and acetonilrile in gradient elution mode at the flow rate of 0.5 mL · min-1 and the detection wavelength was set at 255 nm. RESULTS: The standard curves of compounds 1, 2, 3, and 4 showed good linearity in the ranges of 1.223-24.46, 2.016-40.32, 1.875-37.50 and 16.78-335.6 μg · mL-1 with the corresponding average recoveries of 99.5%, 101.5%, 100.9% and 101.2%, respectively. CONCLUSION: The method is precise and highly reproducible, which can be used to simultaneously determine the antioxidant components including 5-hydroxymelhyl furfural, teuhetenone A, tectochrysin, and nootkatone in Alpinia oxyphylla Miq; compounds 2 and 4 are mainly extracted from the seeds, while compounds 1 and 3 come from the seeds and putamens equally. There are significant differences among the samples from different production areas.