ABSTRACT
Objective To investigate the degradation of three kinds of saponins (notoginseng saponin R1, ginsenoside Rg1, and ginsenoside Rb1) from total saponins of Panax notoginseng (tPNS) by intestinal flora in male and female rats in vitro. Methods tPNS were incubated for 24 h with male and female rats intestinal flora separately in vitro under anaerobic environment. The content of three kinds of saponins was measured at different treatment time. Results The results showed that the intestinal flora both in male and female rats all had the degradation action against ginsenosides Rb1, in which the degradation of male rats was slightly faster than that of female rats. But the intestinal flora both in male and female rats had no obvious degradation to notoginseng saponin R1 and ginsenoside Rg1. Conclusion Under the condition of in vitro, tPNS can be degraded by rats intestinal flora, of which Rb1 was degraded most, while notoginseng saponin R1 and ginsenoside Rg1 were more stable.
ABSTRACT
Objective To establish a method of determining effective components in Tangzhiqing Capsule.Methods Gin- seng saponin Rg_1,Rb_1,Re and notoginseng saponin R_1 in the capsule were separated and purified by D_(101)macroporous absorption resin,and then determined by HPLC.Results The linearity arrange of ginseng saponin Rg_1,Re,Rb_1 and no- toginseng saponin R_1 were 1.88～11.28?g,1.76～10.56?g,0.294～1.764?g,0.752～2.256?g and the recov- eries were 101.51%(RSD=0.75%),100.58%(RSD=0.46%),100.29%(RSD=1.01%),98.64% (RSD = 0.73%)respectively.Conclusion The method is simple,feasible and reproducible,and can be used for the determination of effective components in Tangzhiqing Capsule.
ABSTRACT
AIM:To establish a method of determining effective components in Qianjin Shenanning Pills(Flemingia Philippinensis Merr.et Rolfe,Radix et Rhizoma Ginseng rubra,Radix et Rhizowa Notoginseng,etc). METHODS: The contents of ginseng saponin Rg_1,Rb_1 and notoginseng saponin R_1 were determined by HPLC. RESULTS: The four effective components could be separated and purified by macroporous adsorptive resins of D_(101) and n-butanol,and their contents could be determined. CONCLUSION: The method is simple,feasible and reproducibility is good,it can be used as quality control in medicinal preparation.