ABSTRACT
Ochrosia elliptica Labill. is a small shrub belonging to family Apocynaceae and well-known as a promising anticancer agent. Botanical study of the plant was done for the young and old stems, stem bark, and leaves. Laticiferoustubes with yellowish brown content, isodiametric stone cells (sclereids), sclerenchyma (rod-like), and calcium oxalateclusters are the main diagnostic elements observed in this plant. Furthermore, DNA fingerprinting was done usingrapid amplified (RAPD) and inter simple sequence repeat (ISSR) techniques with the identification of a total of 30RAPD markers and 17 ISSR markers. Carbohydrates, sterols, catechol tannins, flavonoids, and alkaloids were presentin all the organs under investigation. This study could be valuable for quality control of the plant.
ABSTRACT
Objective: To study the non-alkaloid constituents from stems and leaves of Ochrosia elliptica. Methods: The chemical constituents from O. elliptica were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatography, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data in literature. Results: Fifteen compounds were isolated from the ethyl acetate fraction of 90% ethanol extract from O. elliptica, which were identified as hedyotisol A (1), (-)-(7R,7'R,7″S,8S,8'S,8″S)-4',4″-dihydroxy-3,3',3″,5-tetramethoxy-7,9',7',9-diepoxy-4,8″-oxy-8,8'-sesquineolignan-7″,9″-diol (2), 8-hydroxypinoresinol (3), lyoniresinol (4), balanophonin (5), formononetin (6), vestitol (7), mucronulatol (8), (3R)-violanone (9), 6-hydroxy-2-(2-phenylethyl) chromone (10), 6-hydroxy-2-[2-4'-(methoxyphenyl) ethyl] chromone (11), 4-hydroxyacetophenone (12), dehydrovomifoliol (13), loliolide (14), and 4,5-dihydroblumenol A (15). Conclusion: All compounds are isolated from the genus Ochrosia Juss. for the first time.