Clinical Value of serum CA125 ,CA72-4 and TSGF in ovarian cancer / 中国基层医药

Objective To investigate the clinical value of combined detection of the serum levels of CA125,CA72-4 and tumor specific growth factor(TSGF)in the diagnosis of ovary cancer and the curative effect.Methods Serum levels of CA125,CA72-4 and TSGF in 68 patients with ovary cancer,53 patients with benign ovary rumor and 50 normal controls were measured by chemiluminescent immunoassay(CLIA)and chemical colorimetry.Results and clinical data were statisticaly analyzed.Results The levels of CA125,CA72-4 and TSGF in ovary cancer group were higher than that in the benign ovarian tumor group and healthy controls(all P ＜0.01).When use three tumor markers united detection,sensitivity,specificity and accuracy were 91.2％,83.0％ and 87.4％ respectively.The sensitivity and accuracy was higher than any single detection.The specificity with single CA125 testing was consistently.The levels of CA125,CA72-4 and TSGF in patients with ovary cancer were significantly different after surgical treatment for five days(all P ＜0.05).Conclusion The combined detection of the three markers may increase the positive rate in the early diagnosis of ovarian cancer,and help to differentiate the benign and malignant ovarian tumor,but also is valuable to observe the curative rate and postoperative monitor of the ovary cancer.

Ovarian juvenile granulosa cell tumor: a clinicopathological study of 8 cases / 临床与实验病理学杂志

Purpose To investigate the clinicopathologic features, diagnosis and differential diagnosis of ovarian juvenile granulosa cell tumor (JGCT).Methods The history records, pathologic features and immunophenotype of 8 cases of JGCT were retrospectively evaluated and their prognosis was achieved by follow-up.Results The age of patients ranged from 6～21 years old,with an average age of 15.1 years.The main clinical manifestations included an abdominal mass, ascites and isosexual pseudoprecocity. Cut surface of the tumor was typically solid with cysts formed. The histopathological changes displayed solid nests, diffuse sheet, multiple round or ovoid follicles in variable size.Macrofollicles could be seen in some cases.The follicular pattern consisted of small cystic cavities containing eosinophilic secretions. The tumor cells were round or polygonal, medium in size. The tumor cells had abundant pale or slightly eosinophilic cytoplasm, round nuclei with fine chromatin. Nuclear grooves were inconspicuous.Mitosis figures could be found. Immunohistochemical results showed that the tumor cells expressed inhibin-α,CD99,vimentin; while Melan-A,calretinin and S-100 were positive staining in part of the cases.CKpan,EMA,PLAP,Syn and CgA were negative in all the cases.Conclusions Ovarian juvenile granulosa cell tumor is a rather rare, low malignant tumor with good prognosis. Its diagnosis depends on the histologic and immunohistochemical findings and clinical features. Its differential diagnosis includes adult granulose cell tumor, hypercalcaemic type small cell carcinoma, carcinoid and dysgerminoma.

Effect of anti-HER-2 engineered antibody chA21 on angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells / 临床与实验病理学杂志

Purpose To investigate the effect of anti-HER-2 engineered antibody chA21 on the angiogenesis in nude mice xenografts of human ovarian cancer SKOV3 cells that overexpresses HER-2.Methods An animal model with SKOV3 cells involved in nude mice was established and the mice were randomized into control group and chA21 group;chA21 was administrated via caudal vein for 5 weeks, meanwhile the same volume of chA21 buffer was injected in control group.The mice were terminated after 5 weeks and the xenografts dislodged,VEGF and DLL4 expression in two groups were quantitatively analyzed by immunohistochemistry on tissue microarray sections combined with a micro-image analyzing system, and microvessel density (MVD) values were compared between the two groups by immunohistochemical staining of CD31.Results The expression of VEGF, DLL4 and MVD values in chA21 group were lower than those in control group (P<0.05).Conclusion chA21 inhibits the angiogenesis of transplantations of human ovarian cancer SKOV3 cells effectively.

Suppression of insulin-like growth factor-1 receptor by RNA interference inhibits cell growth in vitro and induces chemosensitization of HO8910PM cell to cisplatin / 中华妇产科杂志

Objective To assess the effect of suppression of insulin-like growth factor-1 receptor (IGF1R)in HO8910PM cell line by small interference RNA(siRNA).Methods Transfection of siRNA using liporectamine 2000 was conducted to silence IGF1R gene expression,the expression levels of IGF1R mRNA and protein were evaluated,and the effects on the cell cycles at 48 hours of transfection were assessed by real-time PCR,western blot and flow cytometry(FCM)assay respectively.The cell growth was detected by cell counting kit-8(CCK-8)at 24,48,72,96 hours of transfection.After 24 hours of transfection,the cells were cuhured with difierent concentrations of cisplatin(DDP)for 24 hours,the cell growth inhibition rate Was evaluated by CCK-8.Following incubation with 10μg/ml DDP for 24 hours after 24 hours of transfection,the apoptosis cells and the protein expression level of apoptosis-related gene,B cell leukemia/lymphoma 2(Bcl-2),were identified by FCM and western blot respectively.Resuits (1)Expression levels of IGF1R mRNA and protein were markedly decreased respectively at 48 hours of transfection IGF1R siRNA.(2)Suppression of IGF1R accompanied the reduction of cell growth at 48,72,96 hours of transfection with IGF1R siRNA,absorbance were 1.71±0.13,2.32±0.23,2.79±0.28 respectively (P＜0.01).(3)IGF1R siRNA induces arrest of G2 phase,the G2 phase rate of cells were 24.37%(P＜0.05).(4)Following treatment with 2.5,5,10,20μg/ml DDP for 24 hours after 24 hours of transfection,the cell growth inhibition rates were(25.94±0.08)%,(40.25±0.05)%,(59.48±0.03)%and(74.18±0.08)%respectively(P＜0.01).(5)Treatment with 10μg/ml DDP for 24 hours after 24 hours of transfection,induces 17.95%of cells apoptosis(P＜0.05),and decreases Bcl-2 protein level.Conclusion RNA interference of IGF1R gene induces the IGF1R silence in HO8910PM cell line significantly,inhibits cell growth in vitro, arrests the G2 phase, and enhances the chemosensitization to DDP.

Rectal Teratoma coexistent with an Ovarian Teratoma: A Case Report

We report a case of rectal teratoma coexistent with an ovarian teratoma. To our knowledge, this is the first radiologic report of rectal teratoma. Computed tomography (CT) showed a sharply demarcated cystic and fatty mass with amorphous calcification in the rectum. A double-contrast barium study showed a well-defined intraluminal rectal mass without mucosal destruction. Imaging findings of rectal teratoma allow for correct preoperative diagnosis. CT was helpful in differentiating rectal teratoma from other rectal lesions.

Ovarian Involvement of Peripheral T-Cell Lymphoma as Initial Manifestation of Extranodal Disease

We report here on a case of peripheral T-cell lymphoma of the ovary as the initial manifestation of extranodal disease. A 45-year-old woman presented with an incidentally detected, ovarian mass. Contrast-enhanced CT scans showed a 6x5.3 cm diameter, heterogeneously enhancing mass in the right adnexa that invaded the adjacent uterus. An exploratory laparotomy revealed a solid mass in the right adnexa, which invaded the uterine cornus. Histological diagnosis was made as a peripheral T-cell lymphoma of the ovary.

MR Imaging of Pregnancy Luteoma: a Case Report and Correlation with the Clinical Features

We report here on a 26-year-old pregnant female who developed hirsutism and virilization during her third trimester along with a significantly elevated serum testosterone level. Abdominal US and MR imaging studies were performed, and they showed unique imaging features that may suggest the diagnosis of pregnancy luteoma in the clinical context. After the delivery, the serum testosterone level continued to decrease, and it returned to normal three weeks postpartum. The follow-up imaging findings were closely correlated with the clinical presentation.

Usefulness of Apparent Diffusion Coefficient in Ovarian Cystic Tumors Using Diffusion-Weighted Magnetic Resonance Imaging

PURPOSE: To evaluate the usefulness of apparent diffusion coefficient (ADC) values using diffusion-weighted magnetic resonance imaging (DWI) in patients with ovarian cystic tumors. MATERIALS AND METHODS: During past 12 months, we studied 30 patients who were clinically suspected of having ovarian cystic tumors and who underwent DWI using a 1.5 T MR unit. Eight patients with small cystic ovarian lesions of less than 3 cm in diameter and insufficient DWI were excluded from the calculation of the ADC values. The remaining twenty-six cystic ovarian lesions in 22 patients were classified into four groups; ovarian cysts, cystadenomas, other benign tumors, and malignant tumors. DWI was obtained using single-shot spin echo planar imaging and two gradient steps (b values of 0, 800 sec/mm2). The ADC values were measured using regions-of-interest (ROI) in the cystic components of the DWI located in the same section as the T2-weighted image and away from the septation and solid components. RESULTS: The mean ADC values were 0.196+/-0.105x10(-3) mm2/sec in the ovarian cysts, 1.312+/-1.064x10(-3) mm2/sec in the cystadenomas, 0.274+/-0.124x10(-3) mm2/sec in the other benign tumors, and 1.011+/-0.080x10(-3) mm2/sec in the malignant tumors. The differences in the ADC values between the ovarian cysts and cystadenomas, the ovarian cysts and malignant tumors, the cystadenomas and other benign tumors, and the other benign tumors and malignant tumors were statistically significant (p 0.05). CONCLUSION: The calculated ADC values using DWI should be helpful in the differential diagnosis of cystic ovarian tumors.

Detection of Contralateral Normal Ovary in Patients with Large(>10 cm) Unilateral Ovarian Mass by Using MRI

PURPOSE: To assess the usefulness of MRI for determining the presence of a large (>10 cm) unilateral ovarian tumor by detecting the existence of a normal contralateral ovary, and to establish the difference in detection rates between premenopausal and postmenopausal women, and benign and malignant tumors. MATERIALS AND METHUDS: Materials and Methods: Forty-two patients who underwent MR imaging and in whom the intraoperative gross and pathologic findings indicated the preseace of a unilateral ovarian mass and a normal contralateral ovary were included in this study. The images obtained were retrospectively analyzed by two radiologists, who determined the detection rate of the normal contralateral ovary and whether this differed between premenopausal and postmenopausal women, and benign and malignant tumors. RESULTS: Contralateral normal ovaries were detected in 35 (83.3%) of 42 patients [22 of 23 ovaries (95.7%) in premenopausal women and 13 of 19 (68.4%) in postmenopausal women], with a statistically significant difference (p=0.018). Twelve of 16 of these ovaries (75%) were present in women with malignant tumors, and 23 (88.5%) of 26 in those with benign tumors, but the difference was not statistically significant (p=0.256). CONCLUSION: MR imaging is useful for detecting a normal contralateral ovary and for determining the site at which a large (>10 cm) unilateral ovarian tumor originates, especially in premenopausal women.

Effect of VEGF-C ASODN on adhesion and invasion of ovary cancer cells / 重庆医科大学学报

Objective:To investigate the effect and the mechanism of VEGF-C antisense oligodeoxynucleotide(ASODN) on the adhesion and invasion of human ovary cancer cell line SKOV3 in vitro.Methods:The ASODN sequence targeting the coding region of VEGF-C was synthesized and introduced into ovary cancer cells by liposomes.The expression of VEGF-C protein was detected by inmmnohistochemistry;the adhesion of SKOV3 cells to artifical basement membrane Matrigel was measured by MTT assay.The invasion was evaluated by transwell chambers attached with polycarbonate filters and reconstituted basement membrane(Matrigel).The expression of MMP-2 protein was measured by western blot.Result:In ASODN group,the adhesive rate and the invasive cell numbers were decreased markedly(P

MR Findings of Malignant Struma Ovarii Associated with Mature Cystic Teratoma of Contralateral Ovary: Case Report

Malignant struma ovarii is a rare form of ovarian carcinoma. Sincc it is often seen as a nonspecific complex mass, its preoperative diagnosis is difficult. The MR imaging findings of benign struma ovarii have been reported, but no published account has described the malignant form. In this case, MR images depicted multiple cysts with well enhanced solid portions in the left ovary, and the typical apperarance of mature cystic teratoma in the right ovary. We describe a case of malignant struma ovarii associated with mature cystic teratoma of the contralateral ovary.

Sclerosing Stromal Tumor of the Ovary: MR-Pathologic Correlation in Three Cases

Sclerosing stromal tumor (SST) of the ovary is a very rare sex cord stromal tumor occurring in a younger age group than other types of stromal tumors and most commonly accompanied by menstrual irregularity. Several unique histologic features including pseudolobulation, sclerosis and prominent vascularity are clearly reflected at ultrasonography and MRI. We report the ultrasonographic and MR features of three cases of histologically confirmed SSTs, and relate them to the pathological findings.

In-vivo Proton Magnetic Resonance Spectroscopy in Adnexal Lesions

OBJECTIVE: To explore the in-vivo 1H- MR spectral features of adnexal lesions and to characterize the spectral patterns of various pathologic entities. MATERIALS AND METHODS: Thirty-one patients with surgically and histopathologically confirmed adnexal lesions underwent short echo-time STEAM (stimulated echo acquisition method) 1H- MR spectroscopy, and the results obtained were analysed. RESULTS: The methylene present in fatty acid chains gave rise to a lipid peak of 1.3 ppm in the 1H- MR spectra of most malignant tumors and benign teratomas. This same peak was not observed, however, in the spectra of benign ovarian epithelial tumors: in a number of these, a peak of 5.2 ppm, due to the presence of the olefine group (-CH=CH-) was noted. The ratios of lipid peak at 1.3 ppm to water peak (lipid/water ratios) varied between disease groups, and in some benign teratomas was characteristically high. CONCLUSION: An intense lipid peak at 1.3 ppm is observed in malignant ovarian tumors but not in benign epithelial tumors. 1H- MRS may therefore be helpful in the differential diagnosis of adnexal lesions.

Sclerosing Stromal Tumor of the Ovary: A Case Report

Sclerosing stromal tumor of the ovary is a rare benign neoplasm, with distinctive clinical and pathologic features. It occurs predominantly in females during the second and third decades of life. Histologically, it is composed of cellular and acellular collagenized areas, and edematous stromal areas, and at ultrasonography and computed tomography is seen as a distinctive mixed solid and cystic mass lesion. We report a case of sclerosing stromal tumor of the ovary in a 15-year-old girl with a history of menorrhagia since menarche. Ultrasonography revealed the tumor as a well-defined, lobulated, heterogenous echogenic pelvic mass, while at CT, a huge pelvic mass 9x9x10 cm in size, was seen. This comprised a well-enhanced internal solid portion, a capsule, septa, and a non-enhanced cystic portion.

The comparative study of MRI appearances of ovarian thecoma with pathologic findings / 中华放射学杂志

Objective To analyze and compare the MRI appearances of ovarian thecoma with pathologic findings in order to improve the knowledge of the disease.Methods Nineteen cases of ovarian thecoma confirmed by histopathology were analyzed.MRI morphological characteristics and signal intensity of the lesions were observed and compared with findings of pathomorphology.Correlation analysis between tumor size and amount of ascites was made.Results Ovarian thecoma displayed iso-or hypointense signal on T_1WI and significant hypointensity in the focal lesion on T_2WI.Hyperintensity occurred when cystic degeneration of the lesions existed.Fibrous septation was detected in some lesions.After enhancement,most lesions showed mild early enhancement with slight increase on the delayed phase.Pathological necrosis and cystic degeneration were seen in 9 cases which corresponded to the number and shape of the cystic lesions on MRI.A large amount of collagen hyperplastic was found between the oncocytes microscopically in 15 cases, which displayed significant hypointensity in the focal lesion on T_2WI;another 4 cases showed relatively less amounts of collagen hyperplastic and more oncocytes,which appeared moderate intensity in the focal leisom on T_2WI.The amount of ascites was not significantly correlated with the lesion size(r=0.43,P=0.10). Conclusions Hypointensity on T_2WI and mild enhancement pattern due to poor blood supply are the characteristics of ovarian thecoma.The MR findings can reflect the pathologic features of the tumor,which is helpful in the diagnosis and differential diagnosis.

Primary Ovarian Leiomyoma with Cystic Degeneration: A Case Report

Leiomyoma of the ovary is a very rare benign tumor which is usually found incidentally on routine pelvic examination, or during surgery or autopsy. We report a case of ovarian leiomyoma in a 46-year-old woman in whom a lower abdominal mass was detected. A multiloculated and multiseptated mainly cystic mass in the left adnexa was revealed by computed tomography, and was thought to be an ovarian mucinous cystadenoma. Surgical pathology, however, demonstrated that the mass was a vascular leiomyoma originating from the left ovary.

Radiologic Findings of Malignant Tumors Arising from Ovarian Endometriosis

PURPOSE: To determine the radiologic characteristics of malignant tumors arising from ovarian endometriosis. MATERIALS AND METHODS: The radiologic findings of eleven patients with pelvic masses histologically confirmed as malignant ovarian tumors arising from endometriosis were retrospectively reviewed. All patients underwent MR, and six underwent ultrasonography. The findings were evaluated with regard to tumor size and shape, locularity, thickness and enhancement of the wall and septa, the presence of papillary nodule or solid portion, signal intensity of the locule, the presence of mass in contralateral ovary, ascites, local invasion, distant metastases, and the Pathologic diagnosis included clear cell carcinoma in six cases, endometrioid carcinoma in three, and mucinous cystadenocarcinoma of borderline malignancy and endometrial stromal sarcoma in one case each. RESULTS: Tumor size ranged from 8 to 20 (mean, 12.7) cm. The tumors were mixed in four cases, entirely cystic in three, predominantly cystic in three, and predominantly solid in one. Six cases were unilocular and five were multilocular. The wall and septa varied in thickness and regularity and were well enhanced in all but one case. In all cases papillary nodules or solid portions with similar enhancement to uterine myometrium were seen. On T1WI, the signal intensity of fluid was seen to be high in eight cases, low or intermediate in two, and of differing intensity in one. Ten cases showed high signal intensity on T2WI, whereas in one case in which high signal intensity was seen on T1WI, there was low signal intensity(shading). In threee cases the contralateral ovary contained an endometrioma. Other features included ascites in seven cases and peritoneal seeding in one. CONCLUSION: Malignant ovarian tumors arising from endometriosis showed radiologic features of malignancy:they were larger than 10cm, there was enhancement of the wall and septa, and a papillary nodule or solid portion was present. However, the presence of hyperintense fluid, as seen on T1WI, as well as shading, differing signal intensity, a non-enhanced wall and septa, and endometriosis on the contralateral ovary suggests that a malignant tumor may arise from an underlying endometrioma.

CT Differentiation of Ovarian Mucinous and Serous Cystadenocarcinoma

PURPOSE: To assess the differences between imaging findings of mucinous and serous cystadenocarcinomas of the ova r y, as seen on computed tomography (CT). MATERIALS AND METHODS: The CT findings of 24 patients with mucinous cystadenocarcinoma (25 tumors) and 26 with serous cystadenocarcinoma (47 tumors) of the ovary were retrospectively analysed. Images were evaluated for tumor size, contour, CT attenuation of locules within the mass, the presence of septal vegetation, the proportion of solid portion within the mass, the presence of calcification, and carcinomatosis peritonei. RESULTS: Mucinous cystadenocarcinomas tend to have a smooth contour (96%), variable CT attenuation of locules (80 %), and even size of locules within the mass (88.0%). Serous cystadenocarcinomas, on the other hand, tend to have an rregular lobulated contour (89.4 %), unevenly sized locules (76.6%), septal vegetation (57.4 %), and a prominent solid portion (59.6%). Bilaterality and carcinomatosis peritonei were more common in serous than in mucinous cystadenocarcinoma. CONCLUSION: Features which are valuable for the differentiation of mucinous and serous cystadenocarcinomas of the ova r y, as seen on CT, are tumor size, contour, varying locule attenuation and size, septal vegetation, a solid portion, bilaterality and peritoneal seeding.

CT Differentiation of Solid Ovarian Tumor and Uterine Subserosal Leiomyoma

PURPOSE: On the basis of CT findings, to differentiate between solid ovarian tumor and uterine subserosalmyoma. MATERIALS AND METHODS: In eight surgically proven cases of solid ovarian tumor and in ten uterinesubserosal myoma patients, contrast-enhanced CT images were obtained. Two genitourinary radiologists reviewed thefindings with regard to degree of enhancement of the mass as compared with enhancement of uterine myometrium,thickening of round ligaments, visualization of normal ovaries, contour of the mass, and the presence of ascitesin the pelvic cavity. RESULTS: Six of eight ovarian tumors but only two of ten uterine myomas were less enhancedthan normal uterine myometrium (p 0.05). The contour of themass was lobulated in two of eight ovarian tumor patients, but in five of ten uterine myoma patients (p>0.05). CONCLUSION: CT findings suggestive of solid ovarian tumor were less contrast enhancement of the mass than ofnormal uterine myometrium, pelvic ascites, and nonvisualization of normal ovary.

MR Findings of a Primary Ovarian Lymphoma

The imaging findings of primary ovarian lymphoma have not been reported, and, we therefore describe the MRI(magnetic resonance imaging) findings of a case of this disease, which manifested as bilateral lobulated solid masses with heterogenous signal intensity and central feeding vessels.