Medication rules of famous Beijing TCM doctor Wang Pei in the treatment of colon cancer based on data mining / 国际中医中药杂志

Objective:To explore the medication rules of Professor Wang Pei in the treatment of colon cancer.Methods:The medical records of Professor Wang Pei's outpatient treatment for colon cancer were collected, entered into the Traditional Chinese Medicine Inheritance Computing Platform V3.0, and the laws of the prescription's nature, flavor, meridian, drug frequency, drug combination, association rules, and cluster analysis were explored.Results:A total of 65 prescriptions were collected, including 150 Chinese materia medica. The medicinal properties of these Chinese materia medica are mainly warm, cold and flat, the tastes were mainly sweet, bitter and pungent, and the meridians were mainly liver, spleen and kidney meridians. Dampness category and blood circulation promoting and blood stasis category were mainly used, and the most frequently used drugs include Poria, Polyporus, Scutellariae Barbatae Herba, Astragali Radix, Curcumae Rhizoma, and the pairs of Polyporus- Poria, Astragali Radix- Astragali Radix Praeparata cum Melle, Scutellariae Barbatae Herba- Curcumae Rhizoma. Twenty nine high-related drug pairs were obtained from association rules, including Polyporus- Astragali Radix Praeparata cum Melle- Astragali Radix, Poria- Scutellariae Barbatae Herba- Polyporus, Scutellariae Barbatae Herba- Curcumae Rhizoma- Polyporus. The cluster analysis showed the combination of 6 types of medicines mainly focuses on strengthening the spleen and removing dampness, nourishing qi and promoting blood, clearing heat and detoxifying, and astringing the intestines to stop bleeding. Conclusion:Professor Wang Pei mostly discusses the treatment of colon cancer from "spleen dampness, rectification deficiency, heat toxin, blood stasis, bleeding", and the treatment is "invigorating the spleen and removing dampness, invigorating Qi and strengthening the body, clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and astringent intestines to stop bleeding", which is in line with the thought of Traditional Chinese Medicine treatment of tumors, strengthening the righteousness and eliminating pathogenic factors, and and can be used for clinical reference.

La resignificación participativa de la fundamentación pedagógica como escenario vital para la construcción del Proyecto Educativo Institucional / Participatory Reunification of Pedagogical Foundation as a Vital Scenario for the Construction of the Institutional Educational Project (PEI)

Resumen La Escuela Normal Superior Pbro. José Gómez Isaza (Antioquia - Colombia) realizó una investigación acción participativa propendiendo por resignificar de la fundamentación pedagógica de su Proyecto Educativo Institucional (PEI). Situación suscitada por la necesidad de formar otro tipo de maestros, con un perfil acorde a las demandas del contexto y las diversas poblaciones con las que se desenvuelven. Después de haber desplegado una ruta metodológica pensada por los actores/investigadores, fue necesaria una nueva fundamentación pedagógica, subyaciendo así una propuesta pedagógica crítico-dialógica que repensó desde categorías potencializadoras elementos básicos del currículo: formación, didáctica, investigación, relaciones pedagógicas y evaluación. Con esta propuesta se entretejió sistémicamente un nuevo PEI.

Abstract Priest José Gómez Isaza Normal High School, Antioquia, Colombia led a participatory action research striving for the redefinition of the pedagogical foundation of its Institutional Educational Project (PEI). This situation sprang from the need to form other types of teachers, according to a profile based on the demands of the context and the various populations with which they develop. After having deployed a methodological route which was designed by the actors/researchers, a new pedagogical foundation was necessary. Thus, underpinning a critical-dialogical pedagogical proposal, which reconsidered from potentializing categories, basic elements of the curriculum: training, didactics, research, pedagogical relations, and evaluation. With this proposal, a new PEI was systematically interwoven.

Formar la subjetividad política para el posconflicto: una apuesta desde el proyecto educativo institucional en perspectiva filosófica y pedagógica / Form Political Subjectivity for Post-Conflict: A Bet from the Institutional Educational Project (PEI) on a Philosophical and Pedagogical Perspective

Resumen El presente artículo tiene como finalidad comprender las relaciones e interrelaciones que se tejen en la configuración de la subjetividad política desde los procesos formativos con asiento en el Proyecto Educativo Institucional (PEI), para un escenario futuro de posconflicto. Los resultados y conclusiones se resumen en que: 1) Se necesita una escuela que sea hospitalaria y de acogida, para que propicie el cuidado de sí y del alter en la formación de la subjetividad política; 2) Es necesario que la escuela reflexione sobre la violencia que ha padecido Colombia, para que los estudiantes tengan cuidado de sí y cuidado del otro; 3) El maestro utilizando el consejo como herramienta pedagógica forma la subjetividad, al mostrar posibles caminos que orienten proyectos de vida; 4) la escuela que forme la subjetividad política desde una pedagogía de la conflanza y seguridad debe alejarse del disciplinamiento.

Abstract The purpose of this article is to understand the relationships and interrelationships, which are woven into the configuration of political subjectivity from the training processes with a seat in the Institutional Educational Project (PEI), for a future post-conflict scenario. The results and conclusions are based on that: 1) A school that is hospitable and host is needed to promote the care of itself and the alternative in the formation of political subjectivity; 2) It is necessary for the school to reflect on the violence that Colombia has gone through, so that students take care of themselves and take care for each other; 3) The teacher, by using advice as a pedagogical tool, forms subjectivity, by showing possible paths, which guide life projects; 4) The school, which forms political subjectivity from a pedagogy of trust and security should move away from disciplining.

TGA/Chemometrics addressing innovative preparation strategies for functionalized carbon nanotubes / 药物分析学报

In this work, functionalized carbon nanotubes (CNTs) using two polyamine polymers, polyethyleneimine (PEI) and polyamidoamine dendrimer (PAMAM), were investigated by thermal analysis in order to address preparation strategies to obtain low cytotoxic compounds with the ability to conjugate micro-RNAs and, at the same time, to transfect efficiently endothelial cells. Thermogravimetric analysis (TGA) was coupled to chemometrics as a novel analytical strategy to characterize functionalized CNTs from different preparation conditions. In particular, two starting materials were considered:very small CNTs and carboxylated CNTs (CNT-COOH) in order to examine the affinity with polymers. Chemometrics permitted to compare results from TGA and to investigate the effect of a number of factors affecting the synthesis of coated nanotubes including a different amount of involved polymer and the time required for the suspension for a satisfactory and reproducible preparation procedure. The results demonstrated the effectiveness of TGA as a tool able to address synthesis of coated CNTs to be employed as efficient drug delivery vectors in biomedical applications.

Un ensayo clínico piloto de la efectividad clínica de dos presentaciones de esomeprazol / Clinical effectiveness of two esomeprazole presentations in a pilot trial

Resumen Introducción: el presente estudio tuvo como fin investigar la efectividad clínica de dos presentaciones de esomeprazol en pacientes con dispepsia de causa no estudiada. Métodos: se realizó un ensayo clínico piloto de dos presentaciones de esomeprazol de 40 mg recibidos diariamente por 28 días. Se eligieron pacientes con diagnóstico de dispepsia no estudiada que asistieron a consulta de gastroenterología en un hospital de referencia. Se evaluaron a los pacientes inicialmente con endoscopia y biopsia, el seguimiento a 2 y 4 semanas con escalas clínicas de síntomas y calidad de vida con cuestionarios validados en español (SODA y QoL-PEI) y eventos adversos. Además, se midieron los niveles de pH gástrico con pH-metrías en 24 horas al día 14 de tratamiento. Se tomaron niveles séricos del medicamento al momento de la evaluación de la pH-metría. Para las escalas clínicas se aplicó un análisis de varianza (ANOVA) de dos factores con medidas repetidas y al encontrar diferencias significativas en los tiempos se realizó una corrección de Bonferroni. Resultados: se aleatorizó un total de 33 pacientes, 16 y 17 pacientes en cada grupo. No hubo diferencias en el porcentaje de inhibición del pH gástrico al día 14 de tratamiento (p = 0,9795). No hubo diferencias en concentraciones de niveles séricos el día 14 (p = 0,2199). No se encontraron diferencias significativas en las escalas de gravedad y calidad de vida en las dos primeras semanas de tratamiento, pero sí en las últimas dos semanas, en las cuales el producto de prueba demostró mayor disminución del dolor (p = 0,0048) y superioridad en conformidad (p = 0,01) en la subescala SODA. No se presentaron eventos adversos serios y no hubo diferencias estadísticas entre la presentación eventos adversos no serios. Conclusiones: los productos de prueba y el de referencia mostraron efectos similares en variables clínicamente relevantes.

Abstract Introduction: This pilot studied the clinical effectiveness of two presentations of esomeprazole in patients with dyspepsia with undiagnosed causes. Methods: We conducted a pilot clinical trial of two 40 mg Esomeprazole presentations. Patients with dyspepsia of unknown cause at a gastroenterology clinic in a referral hospital were included. They received one or the other presentation daily for 28 days. Patients were initially evaluated with endoscopy and biopsy and received follow-up examinations at two and four weeks. Adverse events were recorded, and clinical symptom scales and quality of life questionnaires validated in Spanish (SODA and QoL-PEI) were used. In addition, gastric pH levels were measured continuously for 24 hours on day 14 of treatment. Serum levels of the medication administered were also measured on day 14 of treatment. A two-way repeated measures ANOVA was used to compare mean differences between the two groups. When significant differences in times were found, a Bonferroni correction was made. Results: A total of 33 patients were randomized into two groups: 16 patients in one group and 17 in the other. There were no differences in the percentages of gastric pH inhibition at day 14 of treatment (p = 0.9795). There were no differences in serum level concentrations on day 14 (p = 0.2199). No significant differences were found in severity and quality of life scales in the first two weeks of treatment. However, in the last two weeks of treatment the test product showed a larger decrease in pain (p = 0.0048) and superiority in compliance (p = 0.01) on the SODA subscale. There were no serious adverse events, and there were no statistical differences between the presentations of non-serious adverse events. Conclusions: The Test product and the Reference product showed similar effects on clinically relevant variables.

EL PEI, un instrumento pedagógico que al reflexionar sobre la memoria colectiva del conflicto armado posibilita un aprendizaje en torno al dolor y sufrimiento / The Institutional Educational Project (PEI): A Pedagogical Instrument that when Reflecting about the Collective Memory of the Armed Conflict, Enables Learning Around Pain and Suffering

Resumen Al interpretar el Proyecto Educativo Institucional (PEI) a partir de las categorías de subjetividad política y memoria colectiva del conflicto armado, encontramos que: i) la escuela haciendo uso de los 5 componentes del PEI puede hacer pedagogía de la memoria sobre las dimensiones del conflicto armado, ii) la escuela puede resignificar los actos cívicos creando activi dades pedagógicas que comporten nuevos sentidos sobre la violencia de pais al motivar la reflexión en los sujetos educativos, iv) es posible una educación desde el lenguaje poético como grito y silencio de las víctmas, v) haciendo pedagogía de la memoria del conflicto armado, la escuela puede propiciar la unión en la comunidad educativa en torno al dolor y sufrimiento, posibilitando una reelaboración imaginativa del dolor físico a sufrimiento, constituyéndose este, en un tipo de enseñanza y apredizaje.

Abstract In interpreting the Institutional Educational Project (PEI) from the political subjectivity and the collective memory categories of the armed conflict, we find that i) the school, by using the 5 components of the PEI, can do pedagogy of the memory on the dimensions of the armed conflict; ii) the school can re-signify civic acts, by creating pedagogical activities, which bring new senses about country violence, by motivating reflection in educational subjects; iii) an education is possible from the poetic language, like victims' cry and silence; iv) in the pedagogy of the memory of the armed conflict, the school can foster the union in the educational community around pain and suffering, by enabling an imaginative reprocessing of physical pain to suffering, which becomes a sort of teaching and learning.

Preparation and in vitro evaluation of pH-sensitive charge-reversal nanoparticles loaded with curcumin / 中草药

Objective: To prepare charge-reversal pH-sensitive nanoparticles loaded with curcumin (PCE/Cur NPs), and investigate the optimizing technology, physicochemical characterizations, and inhibitory effect on B16 cell. Methods: The β-carboxyl amidized cationic MPEG-PCL-PEI polymers (PCE) were negatively charged, which were prepared into the negative PCE/Cur NPs with pH dependence. When pH > 7, there was no charge-reversal. When pH < 6, the β-carboxyl amides were hydrolyzed rapidly into corresponding amines. As a result, PCE/Cur NPs became positively charged again. The obtained PCE/Cur NPs were characterized by detection of particle size, morphology study, drug loading, encapsulation efficiency, and release study. The effect of anti-migratory and anti-invasive actions of PCE/Cur NPs on B16 cell was investigated using MTT assays and wound healing test. Results: PCE/Cur NPs dependent on pH charge inversion were successfully prepared. The obtained PCE/Cur NPs were round, and the size was uniform, the adhesion was not found. The Results: showed that the prepared PCE/Cur NPs had the highest DL (8.0 ± 1.0)%, EE (90.0 ± 2.0)%, mean particle size of (80 ± 5) nm, and zeta potential of (-35 ± 5) mV. Within 48 h, the accumulative release rate was (69.2 ± 5.2)% (pH 7.4) and (71.2 ± 4.3)% (pH 5), respectively, and then PCE/Cur NPs released slowly. These Results: by MTT assay and wound healing assay indicated that PCE/Cur NPs not only inhibited the proliferation of B16 cells in a concentration- and time-dependent manner, but also can induce apoptosis. Conclusion: PCE/Cur NPs were prepared successfully, which might have great potential application in drug delivery system.

Fatores associados à amputação não traumática em pessoas com Diabetes Mellitus: um estudo transversal / Factors associated with non-traumatic amputation in people with Diabetes Mellitus: a cross-sectional study

Objetivou-se verificar os fatores associados à amputação não traumática em pessoas com Diabetes Mellitus. Estudo transversal com 212 pacientes de um hospital público do estado da Paraíba. A coleta de dados ocorreu através de entrevista, de junho de 2013 a junho de 2014. As variáveis investigadas foram: ocorrência de amputações, faixa etária, sexo, procedência, anos de estudo, renda familiar e características clínicas. Foram utilizados o odds ratio e o modelo de regressão logística para predizer a probabilidade da variável amputação em função das variáveis explanatórias. Os fatores associados à ocorrência de amputação foram: tempo da doença (p=0,006), tempo de procura por atendimento (p=0,001), gangrena (p=0,001) e tabagismo (p=0,016). Verificou-se um alto risco para amputação associada a: tempo de diabetes, tempo de procura por atendimento, gangrena e tabagismo, chamando atenção para maiores esforços direcionados à identificação da complicação e o precoce referenciamento à atenção de maior complexidade.

The objective of this study was to verify the factors associated with non-traumatic amputation in persons with Diabetes Mellitus. Cross-sectional study with 212 patients from a public hospital of the Paraiba state. Data collection occurred through an interview, from June 2013 to June 2014. The investigated variables were occurrence of amputations, age group, gender, origin, years of study, family income and clinical characteristics. The odds ratio and the logistic regression model were used to predict the probability of the amputation variable according to the explanatory variables. The factors associated with the occurrence of amputation were time of disease (p=0.006), search time for attendance (p=0.001), gangrene (p=0.001) and smoking (p=0.016). There was a high risk of amputation associated with: time of diabetes, search time for attendance, gangrene and smoking, calling attention to greater efforts addressed to identification of the complication and early referral to the care of greater complexity.

Magnetic nanoparticles Fe3O4@PEI induced targeted suicide gene therapy combined with magnetic fluid hyperthermia on hepatoma xenograft / 中国肿瘤生物治疗杂志

@#Objective:To investigate the specific killing effect of magnetic nanoparticles Fe3O4@PEI induced targeted suicide gene therapycombinedwithmagneticfluidhyperthermiaonhepatomaxenograft.Methods:Thesuicidegenetargetinghepatomap[HRE]AFP-HSVTK and tumor cell imaging reporter gene vector p[HRE]AFP-Luc were constructed by sub-cloning gene recombination method, and tested by restriction endonuclease gel electrophoresis. Fe3O4 nano-particles were prepared by co-precipitation method and modified by Polyethyleneimine (PEI) to obtain the magnetic nano-particles Fe3O4@PEI,which could be used as carrier for tumor gene therapy and a medium for magnetic fluid hyperthermia treatment; and the characterization of Fe3O4@PEI was identified by transmission electron microscopy, particle size analyzer and Fourier transform infrared spectroscopy. The reporter genes p[HRE]AFP-Luc were delivered into the nude mice bearing xenografts via tail vein by Fe3O4@PEI, then the bioluminescence signals of mice were observed in an IVIS system.After the treatment of p[HRE]AFP-HSVTK/Fe3O4@PEI, the tumor cell inhibition rate was examined by MTT assay, the cell apoptosis was tested by Flow cytometry, the in vivo tumor development rate and tumor inhibition rate was tested by animal experiment, and the sub-cellular construction of tumor cells was observed by Transmission electron microscopy. Results: Nano-particles Fe3O4@PEI and recombinant vectors p[HRE]AFP-HSVTK and p[HRE]AFP-Luc were successfully constructed; after tale vein injection, image signals were detected only in tumor tissues via IVIS system, but no obvious pathologic damage in other major organs. In the in vitro cell killing test, the cell proliferation inhibition rate and the cell apoptosis rate in combination group was higher than that in hyperthermia treatment group and gene treatment group [inhibition rate: (76.02±7.33)% vs (42.31±4.28)%， (47.76±4.81)%, all P<0.05; apoptosis rate: (34.05±3.41)% vs (14.41±1.55)%， (11.64±1.20)%， all P<0.01]. The in vivo treatment showed that tumor volume development significantly slowed-down and even decreased in combination treatment group, and the tumor mass were significantly smaller than those of the single treatment groups (all P<0.05); and the tumor cell sub- cellular structure showed obvious apoptotic morphology. Conclusion: the suicide gene p [HRE]AFP-HSVTK has specifickillingeffecton hepatomacells,Fe3O4@PEI can be used as effectivegene treatmentcarrierand mediaof magnetic heperthermia treatment; Fe3O4@PEI mediated target treatment combined with magnetic fluid hyperthermia treatment could specificallyinhibitthehepatomaxenograft.

Local Delivery of CTGF siRNA with Poly(sorbitol-co-PEI) Reduces Scar Contraction in Cutaneous Wound Healing

Healing process in scarring inevitably produces a considerable amount of non-organized dense collagen-rich matrix called scar thus impairing the native structure of skin. Connective tissue growth factor (CTGF) overexpression within healing tissues is known to play an imperative role in collagen production stimulated by transforming growth factor-beta in cutaneous wound healing. Undoubtedly, the knockdown of CTGF expression through siRNA-mediated gene silencing could simply impede the scarring process. However, the less stability and low transfection of siRNAs themselves urge a safe carrier to protect and transfect them into cells at a high rate avoiding toxicities. Here, we developed a degradable poly(sorbitol-co-PEI) (PSPEI), prepared by polymerization of sorbitol diacrylate with low molecular weight polyethylenimine, which has high transfection efficiency but low cytotoxicity, and utilized it in siCTGF delivery to silence the expression of CTGF in an animal model of cutaneous wound healing. Unlike contracted scar in normal healing, there was no or less contraction in the healed skin of mice treated with siCTGF using PSPEI. Histologically, the healed tissues also had distinct papillary structures and dense irregular connective tissues that were lacking in the control scar tissues. This study exemplifies a successful treatment of cutaneous wound healing using a polymer system coupled with RNA interference. Hence, the approach holds a great promise for developing new treatments with novel targets in regenerative medicines.

Two new lignans from Gymnotheca involucrata / 中草药

Objective: To study the chemical constituents from Gymnotheca involucrata. Methods: The chemical constituents of G. involucrata were isolated by different column chromatographic techniques. The structures of these compounds were identified by comprehensive analysis of the spectroscopic data. Results: Six compounds were isolated from G. involucrata. The compounds were identified as gymnothelignan Y3 (1), gymnothelignan Y4 (2), gymnothelignan T (3), gymnothelignan N (4), gymnothelignan O (5), and gymnothelignan M (6). Conclusion: All the compounds are isolated from G. involucrata for the first time, and compounds 1 and 2 are two new compounds.

Three Cases of Treating Malignant Lymphoma withLiujun Ermu Decotion Used by ZHANG Pei-tong / 中国中医药信息杂志

Malignant lymphoma comes from the lymphoid tissues, which is closely related to immune. The tumor occurs mainly in lymph nodes, but also can occur in the lymph nodes and non-lymphoid tissues. Western medicine treatment for lymphoma is often recurrence after chemotherapy and radiotherapy. TCM not only can play a role in reducing toxicity and increasing the effect of chemotherapy, but also has a good effect on the prevention and treatment of recurrence and metastasis. Professor ZHANG Pei-tong in tumor department of Guang’anmen Hospital of China Academy of Chinese Medicine Sciences usesLiujun Ermu Decotion, which has very good efficacy for malignant lymphoma. This article concluded the clinical experience of Professor ZHANG Pei-tong in usingLiujun ErmuDecotion.

Observation of effect and psychological intervention on treating postpartum hemorrhage due to uterine atony / 中国生化药物杂志

Objective To investigate the clinical effect of Xinmupei and psychological intervention on postpartum hemorrhage caused by uterine atony. Methods The control group was given hemabate treatment,the study group in the patients with uterine atony postpartum hemorrhage hemabate treatment combined with psychological intervention,treatment records,two groups of patients with postpartum hemorrhage caused by uterine atony incidence of adverse reactions,the data input SPSS software for statistical analysis. Results The clinical total efficiency in study group was 93.62％.Total effective rate of the control group was 76.60％(P<0.05);the incidence of adverse reactions(19.15％)was significantly lower than the control group(38.30％,P<0.05). Conclusion In the treatment of postpartum hemorrhage due to uterine atony,the use of psychological intervention for the patient's actual situation is beneficial to achieve more satisfactory treatment effectiveness and safety.

Clinical effect of psychological intervention intervention on postpartum hemorrhage in women with uterine atony and its influence on quality of life / 中国生化药物杂志

Objective To investigate and analyze the clinical efficacy of psychological intervention intervention in the treatment of uterine atony postpartum hemorrhage and its influence on the quality of life. Methods 100 cases of uterine atony postpartum hemorrhage were selected from our hospital from March 2015 to September 2016. The subjects were randomly divided into the control group and the experimental group, each group had 50 cases. The patients in control group were treated with oxytocin, and the experimental group was treated with mother Xin therapy, combined with psychological intervention, pay attention to the mental health of patients, strengthen the communication and communication with patients, and eliminate the negative emotions of patients. Results After the corresponding treatment, the total effective number of patients in the experimental group was 46 cases, and 4 patients were invalid. In the control group, the total effective number was 36 cases, and 14 patients were invalid. The effective rate of the experimental group was 92.0％, which was significantly higher than that of the control group. The effective rate of the treatment group was 72.0％, which was statistically significant (P<0.05). The total score of quality of life in the experimental group was (75.2 ± 11.2) , significantly higher than that of the control group (64.2± 9.3), with statistical difference (P<0.05). Conclusion The clinical effect of psychological intervention of hemabate in treatment of postpartum hemorrhage maternal, can improve the therapeutic effect to a great extent, improve the quality of life of patients, with further clinical promotion and application significance.

Clinical effect of psychological intervention on postpartum hemorrhage in women with uterine atony / 中国生化药物杂志

Objective To investigate and analyze the clinical efficacy of psychological intervention in the treatment of uterine atony postpartum hemorrhage and its influence on the quality of life. Methods 100 cases of uterine atony postpartum hemorrhage were selected in our hospital from March 2015 to September 2016.The subjects were randomly divided into the control group and the experimental group, 50 cases in each group. The patients in the control group were treated with oxytocin, and the experimental group was treated with Xinmu Pei combined with psychological intervention.The treatment effect and quality of life total score were compared between two groups. Results After the corresponding treatment, the effective rate of the experimental group was 92.0％, which was significantly higher than that of the control group(72.0％), the difference was statistically significant (P<0.05). The total score of quality of life in the experimental group was (75.2±11.2) points, significantly higher than that of the control group (64.2±9.3), the difference was statistically significant (P<0.05). Conclusion The clinical effect of psychological intervention of hemabate in treatment of postpartum hemorrhage maternal, can improve the therapeutic effect to a great extent, improve the quality of life of patients, with further clinical promotion and application significance.

Preparation and in vitro evaluation of rhein-loaded PEG-PCL-PEI nanoparticles / 中国中药杂志

This study was aimed to synthesize the polyethyleneglycol-polycaprolactone-polyethyleneimine (PEG-PCL-PEI) three block polymer material, prepareRhein (RH)-loaded PEG-PCL-PEI nanoparticles(PPP-RH-NPS), and then evaluate their physical and chemical properties and biological characteristics in vitro. PEG-PCL-PEI polymer was obtained by adopting thering-opening polymerization and Michael addition reaction, and their physical and chemical properties were analyzed by using NMR and gel permeation chromatography. PEG-PCL-PEI was then used as the carriers to prepare PPP-RH-NPS by applying spontaneous emulsification solvent diffusion method. The results showed that molecular weight of PEG-PCL-PEI polymer was 9.5×103, and critical micelle concentration was 0.723 mmol•L⁻¹. PPP-RH-NPS had pale yellow, opalescence faade, round and smooth without aggregation, formed of (118.3±3.6) nm in particle size with PDI of (0.19±0.08), Zeta potential of (6.3±1.5) mV, entrapment efficiency of (93.64±5.28)%, and drug loading of (8.57±0.53)%. The accumulative release percentage of PPP-RH-NPS was 75.92% in 48h, and the release profiles in PBS conformed to the Higuchi equation： Q=0.121 6t1/2+0.069 5 (R²=0.887 4), presenting slow release characteristics. Within the scope of the 0-0.05 mmol•L⁻¹, the nanoparticles had no obvious hemolysis on rabbit red blood cells and toxicity on HK-2 cells. In the investigation of uptake efficiency by flow cytometry, nanoparticles can be absorbed into cells quickly and internalized within 30 minutes fully, with a high uptake efficiency. In confocal laser scanning microscope observation, the nanoparticles can escape from lysosome into cytoplasm. Herein, this study synthesized the PEG-PCL-PEI polymer and prepared PPP-RH-NPS successfully; the nanoparticles showed uniform particle size, higher encapsulation efficiency and drug-loading rate, slow release characteristics, quick uptake and internalization, lysosome escape property and good biocompatibility. PPP-RH-NPS will be a promising pharmaceutical formulation for further development.

Chemical constituents in lindenane-type sesquiterpene dimers in roots of Chloranthus multistachys / 中草药

Objective: To study the chemical constituents contained in the ethanol extracts from the roots of Chloranthus multistachys. Methods: Twelve compounds were separated from the roots of C. multistachys and their structures were identified by using various chromatographic techniques. Results: The structures of the 12 lindenane-type sesquiterpene dimmers from the chloroform and ethyl acetate extract in the roots of C. multistachys were identified on the basis of physicochemical properties and spectral data as 9-O-β-glucopyranosylcycloshizukaol A (1), cycloshizukaol A (2), shizukaol B (3), shizukaol C (4), shizukaol D (5), shizukaol F (6), shizukaol G (7), chloramultiol B (8), sarcandrolide B (9), sarglabolide I (10), 8-O-methyltianmushanol (11), and henriol B (12). Conclusion: Compounds 1, 7, and 9-12 are isolated from the roots C. multistachys for the first time and compounds 9 and 10 are obtained from the plants of Chloranthus Sw. for the first time.

Chemical constituents from Gymnotheca involucrata / 中草药

Objective: To study the chemical constituents from G. involucrata. Methods: The chemical constituents of G. involucrata were isolated by different column chromatographic techniques. The structures of these compounds were identified by a comprehensive analysis of the spectroscopic data. Results: Ten compounds were isolated from G. involucrata. The compounds were identified as N-benzoyl-2-hydroxy-2-(4'-hydroxy phenyl)-ethylamine (1), N-benzoyl-2-hydroxy-2-(3',4'-dihydroxy phenyl)-ethylamine (2), 6,9- dihydroxy-4,7-megastigmadien-3-one (3), 4-hydroxybenzyl cyanide (4), adenosine (5), hydroxy dihydrobovolide (5-hydroxyl- 3,4-dimethy-5-pentyl-2 (5H)-furanone (6), curculonone A (7), 3α-hydroxy-5,6-epoxy-7-megastigmen-9-one (8), gymnothelignan J (9), and gymnothelignan I (10). Conclusion: All the compounds are isolated from G. involucrata for the first time.

Study on the Percutaneous Properties of Man-PEI25k Nanocomplex under the Treatment of Microneedles / 中国药房

OBJECTIVE:To study the percutaneous properties of Man-PEI25k nanocomplex under the treatment of microneedles. METHODS:Using fluorescent dye water-soluble carboxyl CdSe/ZnS quantum dot (QD) as model drug, Man-PEI25k/QD and Man-PEI25k/QD nanocomplex with different grafting rates(1∶3,1∶6)were formed through electrostatic adherence with PEI25k and Man-PEI25k. The distribution of QD in the active epidermal layer and dermis of skin were observed by confocal microscopy after the treatment of microneedles,using free QD as control. The accumulative retention amounts of QD in the active epidermal layer and dermis of skin were determined by fluorescence spectrophotometer after PEI25k/QD and Man-PEI25k/QD nanocomplex treated with mi-croneedles. RESULTS:The amounts of Man-PEI25k/QD nanocomplex in active epidermal layer and dermis were significantly higher than that of PEI25k/QD nanocomplex under the treatment of microneedles in vivo;the amounts of Man-PEI25k/QD (1∶6) nanocom-plex in active epidermal layer and dermis of skin were significantly higher than that of Man-PEI25k/QD(1∶3)nanocomplex. In in vi-tro transdermal diffusion experiments,microneedles could increase the retention amounts of nanocomplex in active epidermal layer and dermis of skin significantly. The retention amounts of Man-PEI25k/QD nanocomplex in active epidermal layer were increased by 2 times of that of PEI25k/QD under the treatment of microneedles after 48 h;at the same time,in the dermis that was increased by 1.5 times,with statistical significance (P<0.01). CONCLUSIONS:Microneedles can improve the percutaneous properties of Man-PEI25k nanocomplex in active epidermal layer.

Influence on Main Symptoms of Qing-Fei Pei-Yuan Wei-Wan for 141 Pulmonary Infection HIV/AIDS Cases with the Syndrome of Phlegm-heat Obstructing the Lung / 世界科学技术-中医药现代化

This study was aimed to observe influence on clinical symptoms of Qing-Fei Pei-Yuan Wei-Wan (QFPY-WW) for pulmonary infection HIV/AIDS cases with the syndrome of phlegm-heat obstructing the lung. A total of 141 pulmonary infection HIV/AIDS cases with the syndrome of phlegm-heat obstructing the lung were randomly divided into the treatment group (94 cases) and the control group (47 cases). The treatment group was given basic western medication combined with QFPYWW. The control group was given basic western medication. After 4 weeks treat-ment, observation was made on changes of main symptoms. The results showed that on the 28th day, compared with the control group, there was improvement on symptoms such as cough, breathing and chest tightness (P< 0.05). Com-parison on coughing up phlegm degree showed that the treatment group was significantly better than the control group (P< 0.05). On the 7th day treatment, the treatment group had better effect on lowering body temperature than the control group (P< 0.05). It was concluded that QFPYWW can improve symptoms such as cough, breathing, chest tightness and cough up phlegm among pulmonary infection HIV/AIDS patients.