ABSTRACT
PURPOSE: Multiple allergosorbent test-chemiluminescent assay(MAST-CLA) is a semiquantitative method for detecting total IgE and specific IgE in serum. The purposes of this study are to investigate the correlation between MAST-CLA and Paper radioimmunosorbent test(PRIST), and to find out whether MAST-CLA can replace PRIST in measuring of serum total IgE. METHODS: Data of 162 subjects were analyzed by linear regression analysis for MAST-CLA (MAST class) and PRIST(PRIST IgE, PRIST class, PRIST log10 IgE). Diagnostic sensitivity, specificity, efficiency, positive and negative predictive values were calculated by standard methods. RESULTS: The sensitivity of MAST-CLA was 64.0%, specificity was 56.0%, efficiency was 56.0%, positive predictive value was 87.2% and negative predictive value was 21.8%. The overall correlation coefficiency(r) of total IgE between MAST class vs PRIST IgE was 0.176(P<0.05), MAST class vs PRIST class was 0.308(P<0.001), MAST class vs PRIST log10 IgE was 0.301(P<0.001). According to age classification, good correlation was obtained in the 3-6 yr age group(MAST class vs PRIST IgE : r=0.283, P<0.01, MAST class vs PRIST log10 IgE : r=0.377, P<0.001), but there were no significant correlation in the 7-10 yr age group and in the 11-15 yr age group. CONCLUSION: A different correlation was observed between the two tests according to age, so we suggest that simultaneous use of PRIST should be performed considering the age when MAST-CLA is used as an atopy screening.
Subject(s)
Humans , Classification , Immunoglobulin E , Linear Models , Mass Screening , Sensitivity and SpecificityABSTRACT
The aim of the present work is to evaluate the most accurate technique for analysirig low levels of serum IgE. Following results are obtained from experiments by PRIST, Overnight PRIST, PRIST using RAST anti-IgE tracer and Overnight PRIST using RAST anti-IgE tracer methods with cord serum and maternal serum of 21 individuals respectively. 1) There was no statistical significance in maternal serum IgE titer using above 4 methods. However, the higher level of IgE titer was found in PRIST method when IgE was over 190 IU/ml(5 cases) estimated by PRIST method and in Overnight PRIST using RAST anti-IgE tracer method when Ig.E was below lgp IU/ml (16 cases) (p40.05). 2) In cord serum IgE titer, only Overnight PRIST using RAST anti-IgE tracer method showed statistical significance. 3) The most desirable correlation coefficient between maternal serum and cord serum IgE titer was estimated by Overnight PRIST using RAST anti-IgE tracer method than any other rnethods.