ABSTRACT
Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for determining contents of six gastrodin compositions in Gastrodia elata, gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C and parishin E. Methods Separation was carried out on Agilent Zorbax SB-C18 Column (250 mm × 4.6 mm, 5 μm) by using acetonitrile (A)-0.05% Phosphoric acid aqueous solution (B) as the mobile phase, and a linear gradient elution was employed (0-5 min, 2% A, 5-20 min, 2%-30% A, 20-25 min, 30%-2% A) with detection wavelength at 220 nm, flow velocity at 1.0 mL/minand column temperature at 25 ℃. Five relative correction factors (RCFs) of the five compositions using gastrodin as the internal standard were applied to determine their contents in all samples It was established to determine the contents of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E by the external standard method (ESM) at the same time. The feasibility and accuracy of QAMS method were verified by comparing the content results determined by ESM and QAMS. Results Within the linear range, the RCFs of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E were 0.663, 1.278, 1.435 and 1.591, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. Conclusion The QAMS method is feasible and accurate for the determination of the six chemical compositions, and which can be used for quality control of G. elata.
ABSTRACT
Objective: To optimize the integrative technology of primary processing for Gastrodiae Rhizoma by response surface methodology. Methods: The single factor experiment combined with Box-Behnken design was used to optimize the integrative technology, with five major characteristic components (gastrodin, parishin A, parishin B, parishin C, and parishin E) as indexes, in order to detect three factors (steaming time, drying time, and drying temperature), and optimize the primary processing of Gastrodiae Rhizoma. Results: Optimum percolation process was as follows: Gastrodiae Rhizoma was steamed for 30 min, and dried for 12 h at 60℃. Conclusion: This optimized integrative technology of Gastrodiae Rhizoma is reasonable and feasible, and with high accuracy. It could provide the scientific basis and innovative idea to the large-scale production of decoction pieces of Chinese Materia Medica.