ABSTRACT
In order to reveal the relationship between the amount of soil microorganisms and the quality of Fritillaria taipaiensis, both cultivated and wild F. taipaiensis were collected from Chongqing, Wuxi at different stages of their growth as objects of the research. The mycorrhizal infection rate and colonization intensity, peimisine and total alkaloid content in bulbs, the amount of microorganisms and biomass carbon content in rhizospheric soil were all determined using common methods. The results showed that the typical arbuscular-vesicle roots were formed after the AM fungi infected the F. taipaiensis roots which were collected from different origins. The mycorrhizal infection rates were ranged from 78.74% to 98.68% and the colonization intensities were ranged from 13.29% to 37.06%. The rhizospheric microorganisms of F. taipaiensis showed abundant resources. The distribution rule of them in the rhizospheric soil was as follows: the amount of bacteria>the amount of actinomycetes>the amount of fungi. The rhizospheric bacteria, decomposition inorganic phosphorus bacteria, decomposition organic phosphorus bacteria, actinomycetes amount and the total number of microbes increased first and then decreased with the increase of years, while decomposition potassium bacteria showed decreasing trend and fungi showed gradual increasing trend. The soil microbial flora content in the soil changed from "bacterial type" with a high fertility to "fungal type" with a low fertility. The mass fraction of peimisine and total alkaloid content increased first and then decreased with the increase of over the years, the same trend of culturable rhizosphere soil bacteria and actinomycetes indicated that the growth years affected the quality of soil and medicinal materials on different levels. Therefore, the diversity of microbial communities in rhizosphere soil reduced with the increase of years leading to the continuous cropping obstacles and the destruction of medicinal quality of F. taipaiensis.
Subject(s)
Alkaloids , Fritillaria , Chemistry , Microbiology , Mycorrhizae , Plant Roots , Rhizosphere , Soil MicrobiologyABSTRACT
Objective: To develop an HPLC-ELSD method for the determination of amygdalin, aucubin, harpagide, peimisine, peimine and peiminine in Keling capsules simultaneously.Methods: An Ultimate XB C 18 (250 mm× 4.6 mm , 5 μm) chromatographic column was adopted with an ELSD (the drift tube temperature was 105℃, the flow rate of nitrogen was 2.0 L·min-1).The mobile phase was acetonitrile-methanol (1∶1) and 0.4% acetic acid solution with gradient elution at a flow rate of 0.7 ml·min-1 , and the column temperature was set at 35 ℃.Results: Amygdalin, aucubin, harpagide, peimisine, peimine and peiminine was linear within the range of 13.56-271.20 μg·ml-1 (r=0.999 2), 8.48-169.60 μg·ml-1 (r=0.999 9), 4.89-97.80 μg·ml-1 (r=0.999 7), 2.66-53.20 μg·ml-1 (r=0.999 4), 1.82-36.40 μg·ml-1 (r=0.999 8) and 2.04-40.80 μg·ml-1 (r=0.999 6), respectively.The average recovery and the corresponding RSD were 97.90% (1.20%), 99.21% (1.62%), 97.68% (0.75%), 98.36% (1.38%), 99.70% (0.79%) and 97.95% (1.56%)(n =6), respectively.Conclusion: The method is simple and specific, and the results are accurate and repeatable.The method is helpful to the quality control of Keling capsules.
ABSTRACT
Objective: To investigate the protective effect of peimisine on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Methods: The rats were divided into control, model, low-, mid-, and high-dose (2.5, 5, and 10 mg/kg) peimisine groups. Hepatic fibrosis models were induced by ip injection of CCl4 in rats once every 3 d for 8 weeks. The rats in the treatment groups were administered four weeks after the model establishment, once daily until the end of the week 4 after the model establishment. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glutamyl transpeptidasecc (GGT), hyaluronie acid (HA), laminin (LN), type III procollagenc (PC-III), and collegen type IV (IV-C) were assayed, and hepatic tissue contents of hydroxyprolinc (Hyp), superoxide dismutase (SOD), and malondialdehyde (MDA) were determined. The effect of peimisine on hepatic fibrosis in rats was observed. Results: Compared with the model control group, the hepatic fibrosis of rats in peimisine groups was improved obviously, the levels of ALT, AST, ALP, and GGT in serum were lowered obviously (P < 0.05, 0.01), also the serum levels of HA, LN, PC-III, IV-C, and the contents of Hyp and MDA in liver tissue were decreased (P < 0.01), while the level of SOD was increased (P < 0.01). Conclusion: Peimisine has the protective effect on the experimental hepatic fibrosis formation. The possible mechanisms are associated with inhibiting fibrogenesis and fibrosis accumulation, and decreasing lipid peroxidation.