ABSTRACT
Objective To investigate the chemical constituents of Peperomia blanda. Methods Guided by HPLC detection, integrated methods including vacuum liquid chromatography (VLC), ODS and semi-preparative RP-HPLC were used for separation. The structure was determined by spectral analyses including ESI-MS, 1D NMR, 2D NMR and ECD spectra. Results Four compounds were isolated and identified as (7S,7’S,8R,8 ’R)-7-(5-methoxy-3,4-methylenedioxyphenyl)-7-(4-hydroxy-3,5-dimethoxy-phenyl)-8, 8-dihydroxymethyltetrahydrofuran (1), 7, 8-trans-8,8-trans-7,8-cis-7, 7-(4-hydroxy-3,5-dimethoxy phenyl)-8,8-di-acetoxymethyltetrahydrofuran (2), (+)-(7S, 7S, 8R, 8R)-4, 4-dihydroxy-3, 3, 5, 5-tetramethoxy-7, 9, 7, 9-diepoxylignane (3), and (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one (4). Conclusion Compound 1 is a new tetrahydrofuran lignan, compounds 2-4 were isolated from P. blanda for the first time.
ABSTRACT
Objective To investigate the chemical constituents of Peperomia blanda. Methods Guided by HPLC detec?tion,integrated methods including vacuum liquid chromatography(VLC),ODS and semi-preparative RP-HPLC were used for separa?tion. The structure was determined by spectral analyses including ESI-MS,1D NMR,2D NMR and ECD spectra. Results Four com?pounds were isolated and identified as(7S,7′S,8R,8′R)-7-(5-methoxy-3,4-methylenedioxyphenyl)-7′-(4-hydroxy-3,5-dimethoxy?phenyl)-8,8′-dihydroxymethyltetrahydrofuran(1),7,8-trans-8,8′-trans-7′,8′-cis-7,7′-(4-hydroxy-3,5-dimethoxy phenyl)-8,8′-di?acetoxymethyltetrahydrofuran(2),(+)-(7S,7′S,8R,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7,9′,7′,9-diepoxylignane(3), and(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one(4). Conclusion Compound 1 is a new tetrahydrofuran lignan,compounds 2-4 were isolated from P. blanda for the first time.
ABSTRACT
Objective: To study the chemical constituents from Peperomia dindygulensis. Methods: The chemical constituents in chloroform fraction of ethanol extract from P. dindygulensis were isolated and purified by column chromatography over silica gel column, preparative TLC, and semi-preparative HPLC. Their chemical structures were elucidated on the basis of physicochemical and spectral data. Results: Two new polyketides were isolated from the chloroform extracting fraction of P. dindygulensis and identified as (4S)-1,4-dihydroxy-2-(1′, 13′-diketone-octadec-14′E-ene)-1-cyclohexen-3-one (1) and (4S)-1,4-dihydroxy-2-(1′,14′-diketone-octadec-12′E-ene) -1-cyclohexen-3-one (2). Conclusion: Compounds 1 and 2 are new compounds and named as peperomadinone A and peperomadinone B.
ABSTRACT
Objective To study the chemical constituents of Peperomia dindygulensis. Methods Chromatography was used to isolate and purify the chemical constituents, their structures were identified by spectral analyses. Results Eight compounds were isolated and identified as bis-(2-methoxy-4, 5-methylenedioxy)-benzophenone (Ⅰ), peperomin B (Ⅱ), peperomin C (Ⅲ), 5-hydroxy-4′, 7, 8-trimethoxy flavone (Ⅳ), 5-hydroxy-3′, 4′, 7, 8-tetramethoxy flavone (Ⅴ), 5, 3′-dihydroxy-4′, 7, 8-trimethoxy flavone (Ⅵ), ?-sitosterol (Ⅶ), hexadecanoic acid (Ⅷ). Conclusion Compound Ⅰ is a new compound named as dindygulensin. All compounds, except Ⅴ, are isolated from P. dindygulensis for the first time.