ABSTRACT
OBJECTIVE： To study the improvement effects of Shenshao yiqi yangxue granule on experimental aplastic anemia （AA） in mice and the preventive and therapeutic effects of it on myelosuppressive in mice. METHODS： Totally 72 mice were randomly divided into blank group （normal saline）， model group （normal saline）， positive control group （Yixuesheng capsule， 0.625 g/kg in content）， Shenshao yiqi yangxue granule low-dose， medium-dose and high-dose groups （1.26， 7.56， 15.12 g/kg by crude drug）， with 12 mice in each group. Except for blank group， other groups were given 60Co-γ irradiation combined with intraperitoneal injection of CTX to induce AA model. After modeling， they were given relevant medicine intragastrically once a day， for consecutive 7 d. After 6 days of administration， the mice were placed on a treadmill for exhaustion test， and the exhaustion distance and exhaustion time were measured. After 7 days of administration， the changes of peripheral blood （the number of RBC， WBC， PLT and content of HGB） were determined of mice in each group. The percentage of reticulocyte （Ret） was measured after blood smear， and the number of bone marrow nucleated cells （BMNC） was determined after bone marrow smear. Another 90 mice were collected， grouped and given relevant medicine as above， with 15 mice in each group. They were relevant medicine intragastrically once a day， for consecutive 12 d. After 3 days of administration， except for blank group， other groups were given 5-fluorouracil intraperitoneally to induce the bone marrow suppression model at the beginning of medication. After medication， peripheral hemogram， Ret and the number of BMNC were determined in each group. RESULTS： Compared with blank group， the exhaustion distance and exhaustion time of AA model group were significantly decreased （P＜0.01）， while the number of RBC， WBC， PLT and content of HGB， Ret and the number of BMNC were decreased significantly （P＜0.05 or P＜0.01）. Compared with model group， the exhaustion distance and exhaustion time of positive control group and Shenshao yiqi yangxue granule medium-dose and high-dose groups were increased significantly （P＜0.05 or P＜0.01）； the peripheral hemogram indicators， Ret and the number of BMNC were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and Ret of Shenshao yiqi yangxue granule medium-dose group was increased significantly （P＜0.05 or P＜0.01）. In bone marrow suppression model， compared with blank group， the number of WBC， RBC and PLT， Ret， the number of BMNC were decreased significantly in model group （P＜0.05 or P＜0.01）. Compared with model group， the number of WBC， RBC and PLT， Ret and the number of BMNC in peripheral blood were increased significantly in positive control group and Shenshao yiqi yangxue granule high-dose group， and the number of RBC， Ret and the number of BMNC were increased significantly in Shenshao yiqi yangxue granule medium-dose group （P＜0.05 or P＜0.01）. CONCLUSIONS： Shenshao yiqi yangxue granule has certain improvement effects on AA in mice， and can prevent and treat myelosuppression in mice.
ABSTRACT
Objective: To investigate the effect of spleen polypeptide on myelosuppression induced by cyclophosphamide in mice. Methods: The mice were randomly divided into 6 groups:the normal control group,model group,spleen polypeptide〔25, 50,100 mg/ (kg•d)〕groups and positive drug IL-11〔250 μg/ (kg•d)〕group. Except the normal group,the other groups were intra- peritoneally injected with cyclophosphamide〔100 mg/ (kg•d)〕on the first,fourth,and seventh days. At the same time,the spleen polypeptide dose group was administered by tail vein injection every day. Positive drug group was given IL-11 by daily subcutaneous in- jections for 10 days. The organ index of mice,peripheral blood,bone marrow DNA content,number of nucleated cells,and pathologi- cal structure were measured. Results: Compared with the model group,the spleen polypeptide treatment group could improve the mental state and hair suppleness of myelosuppressed mice. Compared with the model group,the liver index of the spleen polypeptide 25,50 and 100 mg/kg groups were increased from 51.02% to 63.23%,60.06% and 59.93% respectively(all P<0.01),and the spleen index were increased from 2.10% to 6.35%,5.32% and 3.80% respectively(all P<0.01). Compared with the model group, the plasma WBC levels of the spleen polypeptide 25 and 100 mg/kg groups were increased from and 2.01×10 9 /L(P<0.01),the plasma RBC levels of the spleen polypeptide 50 and 100 mg/kg groups were increased from 7.06×10 12 / L to 8.40×10 12 /L and 8.00×10 12 /L(all P<0.01),the plasma HGB levels of the spleen polypeptide 50 and 100 mg/kg were increased from 103.25 g/L to 130.80 g/L and 125.86 g/L(all P<0.01),and the plasma PLT levels of the spleen polypeptide 100 mg/kg group was increased from 423.50×10 9 /L to 1285.40×10 9 /L(P<0.01). Compared with the model group,the DNA content of the bone marrow cells of the spleen polypeptide 50 and 100 mg/kg groups increased from 6.69 μg to 13.47 μg and 17.95 μg(all P<0.01),and the number of nucleated cells in the bone marrow cells increased from 1.82×10 9 /L to 6.38×10 9 /L and 9.90×10 9 /L(all P<0.01). Conclu- sion: Spleen polypeptide could improve the bone marrow suppression induced by cyclophosphamide in mice.
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Objective: To compare the effects of Jiaoai Decoction and Siwu Decoction on peripheral hemogram, immune organ, and energy metabolism enzyme activity in blood deficiency model rats, and investigate the mechanism of Jiaoai Decoction derived as a tonic effect of Siwu Decoction after the change. Methods: Taking every day on the fundus venous plexus put blood 5 mL/kg, 12 d continuous bleeding, to establish the rat model of blood deficiency, the red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), platelet (PLT), immune organ index, and energy metabolism enzyme activity were detected. Results: Compared with the normal control group, RBC, HGB, HCT, and PLT could be significantly decreased (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be decreased significantly (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be enhanced (P2+-Mg2+-ATP enzyme activity could be enhanced (P+-K+-ATP enzyme activity was significantly weaker than that in the mid-dose group of Jiaoai Decoction. Conclusion: Jiaoai Decoction and Siwu Decoction have the effect of enriching the blood in different degrees, Jiaoai Decoction evolved into Siwu Decoction could decrease the efficacy of enriching the blood.
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Objective: To investigate the effects of Rehannia glutinosa decoction (RGD)、 Rehmannia glutinosa polysaccharide (RGP) and Rehmannia glutinosa non polysaccharide (RGNP) on peripheral hemogram in mice. Methods: The first experimental group were continuously given RGD、RGP and RGNP for nine days, and then blood sample was taken from tails for determination of peripheral hemogram. The second group were given RGD、RGP and RGNP for five days, after making the blood difficiency model of cyclophosphamide. Determinating peripheral hemogram of the third group, after that those mice were given cyclophosphamide by 2 days, 4 days, 6 days, 8 days. Results: Peripheral hemogram of the every normal group has no difference. To compare the model of blood difficiency group and the control group, there were significant differences, RGNP can only enhance WBC. Both RGP and RGNP can reduce WBC of the mice model of anti cyclophosphamide, while RGP was superior to RGNP. Conclusion: Rehmannia glutinosa has dual directional adjustment function. RGP is tonifying blood active fraction of RG.