Chromosomes of two Olyra l. species from Misiones, Argentina (Poaceae, Bambusoideae, Olyreae) / Cromosomas de dos especies de Olyra l. de Misiones, Argentina (Poaceae, Bambusoideae, Olyreae)

ABSTRACT The subfamily Bambusoideae comprises three monophyletic tribes, Arundinarieae, Bambuseae and Olyreae. Here we report the gametic number and the chromosomal meiotic behavior of two species belonging to the herbaceous tribe Olyreae, Olyra latifolia and Olyrahumilis. Accessions were collected in Misiones, at Northeastern Argentina. We report a new gametic number for O. humilis, n=18, and we confirmed n=11 for O. latifolia. Chromosomal features, like the basic and gametic chromosome number, are important in understanding the evolution of the Poaceae family, especially in delimiting clades and elucidate inter andintra-clades relationships, and therefore it is necessary to continue producing this type of data.

RESUMEN La subfamilia Bambusoideae comprende tres tribus monofiléticas, Arundinarieae, Bambuseae y Olyreae. Aquí reportamos el número gamético y el comportamiento meiótico de los cromosomas de dos especies pertenecientes a la tribu Olyreae de bambúes herbáceos, Olyra latifolia y Olyra humilis. Las introducciones se recolectaron en la provincia de Misiones, en el noreste argentino. Reportamos un nuevo número gamético para O. humilis, n=18, y confirmamos n=11 para O. latifolia. Los números cromosómicos básicos y gaméticos son importantes para comprender la evolución de la familia Poaceae, especialmente para delimitar sus clados y las relaciones existentes entre ellos, por lo que es necesario continuar produciendo este tipo de datos.

Impact of inactivated SARS-CoV-2 vaccination on embryo ploidy: a retrospective cohort study of 133 PGT-A cycles in China

BACKGROUND: Unsubstantiated concerns have been raised on the potential correlation between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination and infertility, leading to vaccine hesitancy in reproductive-aged population. Herein, we aim to evaluate the impact of inactivated SARS-CoV-2 vaccination on embryo ploidy, which is a critical indicator for embryo quality and pregnancy chance. METHODS: This was a retrospective cohort study of 133 patients who underwent preimplantation genetic testing for aneuploidy (PGT-A) cycles with next-generation sequencing technology from June 1st 2021 to March 17th 2022 at a tertiary-care medical center in China. Women fully vaccinated with two doses of Sinopharm or Sinovac inactivated vaccines (n = 66) were compared with unvaccinated women (n = 67). The primary outcome was the euploidy rate per cycle. Multivariate linear and logistic regression analyses were performed to adjust for potential confounders. RESULTS: The euploidy rate was similar between vaccinated and unvaccinated groups (23.2 ± 24.6% vs. 22.6 ± 25.9%, P = 0.768), with an adjusted ß of 0.01 (95% confidence interval [CI]: -0.08-0.10). After frozen-thawed single euploid blastocyst transfer, the two groups were also comparable in clinical pregnancy rate (75.0% vs. 60.0%, P = 0.289), with an adjusted odds ratio of 6.21 (95% CI: 0.76-50.88). No significant associations were observed between vaccination and cycle characteristics or other laboratory and pregnancy outcomes. CONCLUSIONS: Inactivated SARS-CoV-2 vaccination had no detrimental impact on embryo ploidy during in vitro fertilization treatment. Our finding provides further reassurance for vaccinated women who are planning to conceive. Future prospective cohort studies with larger datasets and longer follow-up are needed to confirm the conclusion.

Citogenética comparativa de Physalis peruviana en tres poblaciones cultivadas de Cajamarca, Perú / Comparative cytogenetics of Physalis peruviana from three cultivated populations from Cajamarca, Peru

Resumen En este trabajo se caracteriza y compara citogenéticamente Physalis peruviana "aguaymanto" de poblaciones cultivadas de la región Cajamarca: San Pablo, Celendín y Cajabamba. El número cromosómico más frecuente en las tres poblaciones fue 2n = 4x = 48 con frecuencias de 60, 50 y 34% en las poblaciones de San Pablo, Celendín y Cajabamba respectivamente. En menor frecuencia se encontró casos de aneuploidía somática. Los resultados permitieron distinguir cada población a partir de su fórmula cariotípica 17m+4sm+3t, 24m y 20m+2sm+2t que identifican a San Pablo, Celendín y Cajabamba respectivamente. Los cariotipos fueron caracterizados por la longitud total del complemento haploide (HCL) y los índices de simetría (S%), asimetría (A) y asimetría intra e intercromosómica (A1 y A2). El mayor valor de HCL se describió en San Pablo. Celendín presentó el mayor grado de simetría (S%=53.226 y A= 0.177), mientras que los cariotipos de San Pablo y Cajabamba fueron descritos como los más asimétricos. Se concluye que la condición más frecuente es la tetraploidía; aunque se evidencia diferente morfología cromosómica entre los cariotipos de las tres poblaciones.

Abstract In this work, we characterized cytogenetically Physalis peruviana "aguaymanto" and cultivated populations of the Cajamarca region: San Pablo, Celendín and Cajabamba are compared. The most frequent chromosomal number in the three populations was 2n = 4x = 48 with frequencies of 60, 50 and 34% in San Pablo, Celendín and Cajabamba respectively. Few cases of somatic aneuploidy were found. Our results let distinguish the populations by its karyotypic formula 17m + 4sm + 3t, 24m and 20m + 2sm + 2t (San Pablo, Celendín and Cajabamba respectively). Karyotypes were characterized by the total length of the haploid complement (HCL) and the indices of symmetry (S%), asymmetry (A) and intra and interchromosomal asymmetry (A1 and A2). The highest value of HCL was described in San Pablo. Celendín presented the highest degree of symmetry (S% = 53.226 and A = 0.177), while the karyotypes of San Pablo and Cajabamba were described as the most asymmetric. We concluded that the tetraploidy is most frequent condition; although there is evidence of different chromosomal morphology between the three populations.

Application of sputum cell DNA ploidy quantitative analysis technique in early non-invasive screening of lung cancer / 肿瘤研究与临床

Objective@#To investigate the application value of sputum cell DNA ploidy quantitative analysis technique in early non-invasive screening of lung cancer.@*Methods@#The clinical data of 84 patients with lung cancer (lung cancer group) and 84 patients with benign lung disease (lung benign disease group) who were admitted to the Second Affiliated Hospital of Xuzhou Medical University from April 2016 to May 2017 were retrospectively analyzed, and 80 healthy subjects were selected as the control group. The sputum samples of all subjects were collected, and 55 corresponding lavage fluid samples in the lung cancer group were also collected. A fully automated cell tumor screening analysis system was used to make DNA ploidy quantitative analysis in all specimens, and the results were compared with sputum smear and liquid-based thin-layer cytology results.@*Results@#The positive detection rates of routine smear, liquid-based thin-layer cytology and DNA ploidy quantitative analysis in lung cancer group were 4.76% (4/84), 29.76% (25/84) and 45.24% (38/84). The positive detection rate of liquid-based thin-layer cytology and DNA ploidy quantitative analysis was higher than that of routine smear, and the differences were statistically significant (χ 2 = 18.38, P < 0.01; χ2 = 36.70, P < 0.01); the positive detection rate of DNA ploidy quantitative analysis was higher than that of liquid-based thin-layer cytology, and the difference was statistically significant (χ 2 = 4.29, P = 0.038). The positive detection rate of DNA ploidy quantitative analysis in lavage fluid samples was higher than that in sputum samples [65.45% (36/55) vs. 50.91% (28/55)], but the difference was not statistically significant (χ 2 = 2.39, P = 0.122). For peripheral lung cancer patients, the positive detection rate of DNA ploidy quantitative analysis was higher than that of liquid-based thin-layer cytology (χ 2 = 4.55, P = 0.033). The positive detection rate of squamous cell carcinoma [52.17% (24/46)] by using DNA ploidy quantitative analysis was higher than that of adenocarcinoma [36.36% (8/22)], small cell carcinoma [26.36% (4/11)] and adenosquamous carcinoma [40.00% (2/5)], but the differences were not statistically significant (all P > 0.05).@*Conclusions@#DNA ploidy quantitative analysis technique of sputum cells can directly reflect the malignant transformation of early lung cancer patients compared with traditional smear method and liquid-based thin-layer technology. Its positive detection rate is higher than that of the conventional detection method, which can be used as a valuable reference index for early screening of lung cancer.

Values of exfoliated cell smear, DNA ploidy analysis, cell block and their combined detection in diagnosis of malignant pleural effusion / 吉林大学学报(医学版)

Objective: To study the values of exfoliated cell smear, DNA ploidy analysis, cell block and their combined detection in the diagnosis of malignant pleural effusion, and to provide the evidence for the diagnosis and treatment of malignant pleural effusion Methods: A total of 300 cases of pleural effusion specimens were analyzed by DNA ploidy analysis to judge the benign and malignant pleural effusion; the centrifuged cell pellet was used for smear, the cell block was made, and the source of malignant pleural effusion was judged by immunohistochemistry method The sensitivities and specificities of three methods and their combined detection in the diagnosis of malignant pleural effusion were compared Results: The sensitivities of exfoliated cell smears, DNA ploidy analysis and cell block in the diagnosis of malignant pleural effusion were 83. 13%, 84. 44%, and 79. 52%, respectively; the specificities were 82. 95%, 86. 64%, and 83. 87%, respectively; the sensitivity of parallel test in the diagnosis of malignant pleural effusion was 98. 79%; the specificity of serial test in the diagnosis of malignant pleural effusion was 99. 54%. Conclusion: The combined detection of three methods can significantly improve the clinical diagnotic effect of malignant pleural effusion compared with single detection of three methods.

Application of sputum cell DNA ploidy quantitative analysis technique in early non-invasive screening of lung cancer / 肿瘤研究与临床

Objective To investigate the application value of sputum cell DNA ploidy quantitative analysis technique in early non-invasive screening of lung cancer. Methods The clinical data of 84 patients with lung cancer (lung cancer group) and 84 patients with benign lung disease (lung benign disease group) who were admitted to the Second Affiliated Hospital of Xuzhou Medical University from April 2016 to May 2017 were retrospectively analyzed, and 80 healthy subjects were selected as the control group. The sputum samples of all subjects were collected, and 55 corresponding lavage fluid samples in the lung cancer group were also collected. A fully automated cell tumor screening analysis system was used to make DNA ploidy quantitative analysis in all specimens, and the results were compared with sputum smear and liquid-based thin-layer cytology results. Results The positive detection rates of routine smear, liquid-based thin-layer cytology and DNA ploidy quantitative analysis in lung cancer group were 4.76％ (4/84), 29.76％ (25/84) and 45.24％ (38/84). The positive detection rate of liquid-based thin-layer cytology and DNA ploidy quantitative analysis was higher than that of routine smear, and the differences were statistically significant (χ2= 18.38, P<0.01;χ2=36.70, P<0.01);the positive detection rate of DNA ploidy quantitative analysis was higher than that of liquid-based thin-layer cytology, and the difference was statistically significant (χ2= 4.29, P= 0.038). The positive detection rate of DNA ploidy quantitative analysis in lavage fluid samples was higher than that in sputum samples [65.45％(36/55) vs. 50.91％ (28/55)], but the difference was not statistically significant (χ2=2.39, P= 0.122). For peripheral lung cancer patients, the positive detection rate of DNA ploidy quantitative analysis was higher than that of liquid-based thin-layer cytology (χ2=4.55, P=0.033). The positive detection rate of squamous cell carcinoma [52.17％ (24/46)] by using DNA ploidy quantitative analysis was higher than that of adenocarcinoma [36.36％ (8/22)], small cell carcinoma [26.36％ (4/11)] and adenosquamous carcinoma [40.00％ (2/5)], but the differences were not statistically significant (all P> 0.05). Conclusions DNA ploidy quantitative analysis technique of sputum cells can directly reflect the malignant transformation of early lung cancer patients compared with traditional smear method and liquid-based thin-layer technology. Its positive detection rate is higher than that of the conventional detection method, which can be used as a valuable reference index for early screening of lung cancer.

Yield, structural composition and nutritive characteristics of ryegrass cultivars used to haymaking in lowland soils / Rendimento, composição estrutural e características nutritivas de cultivares de azevém utilizadas para fenação em solos de várzea

This research aimed to evaluate the dry matter yield, structural composition and nutritive characteristics of diploid and tetraploid annual ryegrass cultivars on different phenological development to haymaking on lowland soils. The experimental design was developed based on randomized blocks with split plots, four cultivars of annual ryegrass (BRS Ponteio and FEPAGRO São Gabriel, diploid; INIA Escorpio and KLM 138, tetraploid), and three phenological crop phases (vegetative, pre-blossoming and blossoming). Were evaluated, dry matter yield, leaf:stem ratio, leaf weight ratio, tiller population density, specific leaf area, crude protein, neutral detergent fiber, and acid detergent fiber. All data were submitted to analysis of variance and the means were compared by Tukey-Kramer test (p< 0,05). Highest forage mass is obtained with harvest in blossoming stage. Tetraploid cultivars present better leaf proportion and higher content of crude protein during vegetative stage. The decrease the in concentration of protein with the change of phenological stage is less evident on diploid cultivars. The vegetative stage enables harvest forage with high nutritional value, with lower production of biomass.

O objetivo do trabalho foi avaliar o rendimento de matéria seca, composição estrutural e características nutritivas de cultivares de azevém anual, diploides e tetraploides em diferentes estádios fenológicos, para fenação, em solos de várzea. O delineamento experimental foi de blocos ao acaso, com parcelas divididas, com quatro cultivares de azevém anual (BRS Ponteio e FEPAGRO São Gabriel, diploides; INIA Escorpio e KLM 138, tetraploides) e três estádios fenológicos (vegetativo, pré-florescimento e florescimento). Foram avaliadas: rendimento de matéria seca, relação folha/colmo, razão de peso foliar, densidade populacional de perfilhos, área foliar específica, proteína bruta, fibra em detergente neutro e fibra em detergente ácido. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste Tukey-Kramer (p <0,05). A maior massa de forragem é obtida com a colheita no estádio de florescimento. As cultivares tetraploides apresentam melhor proporção foliar e maior teor de proteína bruta durante o período vegetativo. A diminuição na concentração de proteína, com a mudança de estádio fenológico é menos evidente nas cultivares diploides. O estágio vegetativo possibilita a colheita de forragem com alto valor nutricional, mas com menor produção de biomassa.

Study of a novel diagnostic assay for thoracic malignant tumor based on DNA image cytometry / 临床与实验病理学杂志

Purpose To explore the effects of ploidy analysis on thoracic neoplasms based on DNA image cytometry (DNA-ICM), and to look for a meaningful novel diagnostic assay for tumor patients. Methods 4 402 patients who were diagnosed with thoracic disease were recruited in 2 years. By the DNA-ICM analysis, all the specimens were diagnosed as three types——positive, equivocal and negative ones. The results of701 specimens were compared with biopsy and clinical followup. Results DNA aneuploidy detected by DNA-ICM were65％ in confirmed malignant samples, 64％ in equivocal malignancy, and 8％ in non-malignant diseases. The comprehensive performance of DNA-ICM in malignancy was 73％, 93％, 71％, 94％ respectively for sensitivity, specificity, positive predictive value and negative predictive value. OR analysis found that the risk ratio of aneuploidy in malignancy was 23.236 compared to non-malignancy. Conclusion DNA-ICM can be applied in thoracic malignancy and have more potential values to be explored in oncology.

Progress and perspectives on cyanobacterial ploidy / 生物工程学报

Cyanobacteria are a phylum of bacteria which are believed to be the oldest photosynthetic prokaryotic microorganisms on earth. The phylogenetic group of cyanobacteria was thought to be one of the prokaryotes that contain monoploid, oligoploid and polyploid species, and one obstacle to engineering cyanobacteria is their polyploidy genome. In recent years, the ploidy level of cyanobacteria was found to be influenced by growth phase and by multiple genetic and environmental factors. In the present article, we reviewed the progress, analytical methods and influencing factors on the cyanobacterial ploidy, and discussed the significance of cyanobacterial polyploidy regarding to environmental ecology and biotechnology. Based on this observation, the future research directions in this field are prospected.

Karyotype analysis in Bignonieae (Bignoniaceae): chromosome numbers and heterochromatin

ABSTRACT Chromosome numbers and heterochromatin banding pattern variability have been shown to be useful for taxonomic and evolutionary studies of different plant taxa. Bignonieae is the largest tribe of Bignoniaceae, composed mostly by woody climber species whose taxonomies are quite complicated. We reviewed and added new data concerning chromosome numbers in Bignonieae and performed the first analyses of heterochromatin banding patterns in that tribe based on the fluorochromes chromomycin A3 (CMA) and 4'-6-diamidino-2-phenylindole (DAPI). We confirmed the predominant diploid number 2n = 40, as well as variations reported in the literature (dysploidy in Mansoa [2n = 38] and polyploidy in Dolichandra ungis-cati [2n = 80] and Pyrostegia venusta [2n = 80]). We also found a new cytotype for the genus Anemopaegma (Anemopaegma citrinum, 2n = 60) and provide the first chromosome counts for five species (Adenocalymma divaricatum, Amphilophium scabriusculum, Fridericia limae, F. subverticillata, and Xylophragma myrianthum). Heterochromatin analyses revealed only GC-rich regions, with six different arrangements of those bands. The A-type (one large and distal telomeric band) were the most common, although the presence and combinations of the other types appear to be the most promising for taxonomic studies.

Application of DNA ploidy analysis in diagnosis of serous cavity effusion / 国际检验医学杂志

Objective To explore the application value of automated cell DNA ploidy analysis system in the diagnosis of benign and malignant serous cavity effusion.Methods 262 cases of serous cavity effusion(169 cases of pleural effusion,78 cases of ascites,15 cases of pericardial effusion)were treated by centrifugation,2 slices of each sample were made.One of them used for dyeing Feulgen,which given automatic cell DNA ploidy analysis,another one for Papanicolaou staining,with a conventional cytology.The positive detection rate of these 2 kinds of different detection methods for malignant serous cavity effusion were compared.Results 119 cases(45.4％)of 262 cases abnormal were detected by conventional cytology of serous cavity effusion.Meanwhile,113 cases (43.1 ％)were detected abnormal by DNA ploidy analysis in the same samples.73 cases of tumor cells and suspicious tumor cells were found by conventional cytology,and different ploidy cells were found in all of these samples In conventional cells,46 cases of nuclear heterogeneous cells were found,while only 34 cases exist different ploidy cells.Conclusion Automated cell DNA ploidy analysis system is helpful to improve the positive diagnosis rate of serous cavity effusion,which can be used as an important auxiliary means of cytology.

Study on immunocytochemistry p16/Ki-67 double staining combined with DNA ploidy analysis for analyzing and predicting cervical high grade squamous intraepithelial lesion / 重庆医学

Objective To investigate the diagnostic value of DNA ploidy analysis combined with immunocytochemistry p16/ki-67 double staining in cervical high grade squamous intraepithelial neoplasia(HSIL) and cervical squamous cell carcinoma(SCC).Methods A total of 73 cases of cytological tests were randomly collected.Among them,53 cases were small DNA ploidy abnormal cells and 20 cases were DNA ploidy negative.The p16/Ki-67 results were detected by immunocytochemistry double staining.With the pathological results as the golden standard,the diagnostic values of DNA ploidy analysis and DNA ploidy analysis combined with p16/Ki-67 double staining in HSIL + was contrastively analyzed by pathologic results.Results Among 20 samples of DNA ploidy negative,the p16/Ki-67 double staining results all were negative.The positive predictive value of DNA ploidy analysis for HSIL + was 34.62％.The sensitivity of DNA ploidy analysis combined with p16/Ki-67 double staining for HSIL + was 84.62％,and its specificity was 92.31％,the positive predictive value was 78.57％ and the negative predictive value was 94.74％,which were significantly higher than those of DNA ploidy analysis(P＜0.05).Conclusion p16/Ki-67 double staining can significantly im prove the prediction value of HSIL.The DNA ploidy analysis combined with p16/Ki-67 double staining is an effective method for predicting HSIL +,which is suitable for the implementation in the areas with lack of medical resources.

Application of DNA-image cytometry in the diagnose of urothelial cell carcinomas / 实用医学杂志

Objective To investigate the value of DNA-image cyt-ometry (DNA-ICM) in the diagnosis of urothelial cell carcinomas (UCC). Methods Totally 162 voided urine specimens (92 cases from urothel-ial car-cinomas patients and 70 cases from benign urinary system diseases patients ) were detected with DNA-ICM and liquid-based cytology (LBC), respectively. Results The sensitivity and specificity of DNA-ICM were 65.2%and 100% respectively in the diagnosis of UCC but those of LBC were 27.2% and 98.6%, respectively. The sensitivity of DNA-ICM was significantly higher than that of LBC in the diagnosis of UCC (P 0.01). Conclusion DNA-ICM, which improves the positive rate of urinary cytology, has great application value in the diagnosis of urothelial cell carcinomas and it is an effective screening method for urothelial cancer in diag-nosis and follow-up.

Effect of amifostine on proliferation and differentiation of human megakaryocyte Dami cells / 中国药理学与毒理学杂志

OBJECTIVE To investigate the effect of amifostine(Amf)on the differentiation of human megakaryocyte cell line-Dami. METHODS Dami cells were treated with Amf 0.01-5.0 mmol · L-1 for 12 d. Dami cells were counted every day for the growth curve:only cells with a diameter>20μm. The platelet demarcation membrane system was observed by transmission electron microscopy. The expression of CD33,CD34,CD41a and DNA ploidy was detected by flow cytometry. RESULTS Amf 0.1-1.0 mmol · L-1 promoted the differentiation of Dami cells ,but inhibited their proliferation at a concentration>1.0 mmol · L-1. When these cells were treated with Amf 1.0 mmol · L-1 for 12 d,the platelet demarcation membrane system was observed,the percentage of cells with a diameter >20 μm was increased by 24.6%(P1.0 mmol·L-1).

Study on micromegakaryocytes of patients with myelodysplastic syndrome by flow cytometry / 重庆医学

Objective To setup a measurement of human bone marrow micromegakaryocyte which based on CD41a and PI double‐labeled flow cytometric analysis ,and study the significance in the diagnosis of MDS .Methods In 42 cases of MDS patients , their bone marrow megakaryocytes were obtained by Percoll density gradient separation medium .The megakaryocyte glycoproteinⅡb/Ⅲa(CD41a)were marked with fluorescein isothiocyanate through its corresponding monoclonal antibody ,and their DNA were marked with PI .Then the megakaryocyte ploidy was analyzed by flow cytometry(FCM ) .Results The method for micromegakaryo‐cyte identification and analysis was established .In 42 patients with MDS ,the detection rate of micromegakaryocyte was 90 .5 per‐cent by FCM analysis ,but only 54 .8 percent by Wright‐Giemsa staining test and 64 .3 percent by immunohistochemistry ,the differ‐ence among them was statistically significant(χ2 = 13 .640 ,P= 0 .001) .The 42 patients with MDS were divided into two groups (low‐risk group and high‐risk group) .The detection rates of micromegakaryocyte were 81 .8 percent in low‐risk group and 100 per‐cent in high‐risk group separately by FCM analysis ,the difference was statistically significant(χ2 =4 .019 ,P=0 .045) .Conclusion The detection rate of micromegakaryocyte by FCM with CD41a and PI double marker is higher than that by cytochemical staining . The detection rate of micromegakaryocyte in the high‐risk group is higher than that of the low‐risk group ,which shows that the de‐tection of micromegakaryocyte is of great significance for MDS prognosis assessment .

The effects of human papilloma infection status on the DNA ploidy of cervical epithelial cells in Uygur and Han women / 重庆医学

Objective To explore the effects of human papilloma(HPV) infection status on the DNA ploidy of cervical epi-thelial cells in Uygur and Han women .Methods Totally 348 women collected who initially received treatment in Tumor Hospital Affiliated to Xinjiang Medical University from July 2012 to June 2014 ,including 181 cases of Uygur and 167 cases of Han women . HPV genotyping was dased on cytology specimens from thin layer of liquid ,and DNA ploidy analyze [DNA index(DI) and S phase cells ratio(SPF)] was conducted by using flow cytometry .All the patients were divided into negative infection group ,non high-risk infection group ,single high-risk HPV infection group and mixed high-risk HPV infection group according to HPV gene type .Re-sults There were statistically significance between Uygur and Han women of DI and SPF in the single high-risk HPV infection group(P= 0 .033 ,P< 0 .01) ,it also present the same trend in mixed high-risk HPV infection group(P = 0 .031 ,P< 0 .01) .It was 19 .783 times and 59 .231 times to appear DNA aneuploid in single high-risk HPV infection and mixed high-risk HPV infection compared to the HPV negative infection group in Uygur women .It was 11 .190 times and 22 .125 times in Han women . Conclusion Single high-risk type HPV infection and mixed high-risk HPV infection had different impact on cervical lesions be-tween Han and Uygur women .It was necessary to respectively study the correlation between cervical lesions and HPV infection for each ethnic groups .

Meta Analysis of FCM DNA Ploidy in Distinguishing Benign Tumor from Malignant Ones / 中国医科大学学报

Objective To evaluate the application value of DNA ploidy detection using flow cytometry method(FCM)in malignant tumor identifi?cation,so as to provide the theoretical basis for the clinical diagnosis of malignant tumors. Methods Two researchers finished the literature screen?ing independently,and all the literatures were given the secondary screening according to the inclusion and exclusion criteria. The included literature data was analyzed by Meta?DiSc 1.4,including heterogeneity test,sensitivity,specificity,diagnostic odds ratio(DOR)and summary receiver operat?ing characteristic(SROC)etc. Results Totally 12 literatures were included in the study finally,including a total of 1 340 subjects consisting of 516 cases with malignant tumor and 824 cases with benign tumor. Heterogeneity inspection results showed that the Spearman correlation coefficient of sensitivity logarithm and(1-specificity)logarithm was-0.343 and there was no threshold effect(P=0.275). DOR curves was Cochran?Q=26.49 (P=0.005 5),indicating the heterogeneity was caused by non threshold effects. Combined statistical quantity was calculated with a random effects model and the results were as followings:the sensitivity was 0.72(95%CI:0.68?0.76,I2=50.1%)and the specificity 0.84(95%CI:0.81?0.86,I2=65.5%). SROC curve drawing,DNA ploidy detection of benign and malignant tumors showed AUC=0.845 3 and Q*=0.776 8. Conclusion FCM DNA heteroploid has a high accuracy for diagnosis of malignant tumor,which can be an important supporting means for the discrimination between benign and malignant tumor.

The study of prognosis value of AFP and DNA ploidy detection in primary hepatic carcinoma / 国际检验医学杂志

Objective To investigate the relationship between the serum level of AFP ,types of DNA ploidy and 5 year survival rate ,survival time in patients with primary hepatic carcinoma (PHC) in Boluo area in Huizhou City ,and evaluate the prognosis val‐ue of these indexes in PHC .Methods 80 cases with PHC were enrolled in observation group and divided into grade I ,II ,III and IV according to pathological grading .Other 80 cases of healthy individuals ,conducted physical examination ,were enrolled in control group .Peripheral venous blood samples (2 mL) were collected from all subjects .The serum level of AFP and types of DNA ploidy were measured by electrochemiluminescence immunoassay (ECLIA) and flow cytometry ,respectively .Results The serum levels of AFP were significantly higher in patients in different pathological grade groups compared to that in the control group (P<0 .05) .In addition ,accompany increase of pathological grade ,the levels of AFP in patients with PHC were increased .The increased level of AFP ,difference of DNA ploid types and pathological grade of PHC contributed great influences on the 5 year survival rate and sur‐vival time of patients .Conclusion The serum level of AFP and the DNA heteroploid rate may play important roles in the early di‐agnosis and assessing prognosis of patients with PHC in Boluo area in Huizhou City .

The parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes / 대한생식의학회지

OBJECTIVE: It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype. METHODS: The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin. RESULTS: 3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos. CONCLUSION: The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.

In vitro regeneration and ploidy level analysis of Eulophia ochreata Lindl.

Various parameters including explant-type, medium compositions, use of phytohormones and additives were optimized for direct and indirect regeneration of E. ochreata, a medicinal orchid under threat. Protocorm-like-bodies (PLBs) proved to be the best explants for shoot initiation, proliferation and callus induction. Murashige and Skoog’s (MS) medium containing 2.5 mg L-1 6-benzylaminopurine (BAP), 1.0 mg L-1 kinetin (Kin) and additives (adenine sulfate, arginine, citric acid, 30 mg L-1 each and 50 mg L-1 ascorbic acid) was optimal for shoot multiplication (12.1 shoots and 7.1 PLBs per explant with synchronized growth), which also produced callus. Shoot number was further increased with three successive subcultures on same media and ~40 shoots per explant were achieved after 3 cycles of 30 days each. Additives and casein hydrolysate (CH) showed advantageous effects on indirect shoot regeneration via protocorm-derived callus. Optimum indirect regeneration was achieved on MS containing additives, 500 mg L-1 CH, 2.5 mg L-1 BAP and 1.0 mg L-1 Kin with 30 PLBs and 6 shoots per callus mass (~5 mm size). The shoots were rooted (70% frequency) on one by fourth-MS medium containing 2.0 mg L-1 indole-3-butyric acid, 200 mg L-1 activated charcoal and additives. The rooted plantlets were hardened and transferred to greenhouse with 63% survival rate. Flow-cytometry based DNA content analysis revealed that the ploidy levels were maintained in in vitro regenerated plants. This is the first report for in vitro plant regeneration in E. ochreata.