ABSTRACT
Objective To study the extraction method in flowers of Polygonum orientale L.. Determine the optimum extraction process and establish a method by HPLC for simultaneous determination of three components. Methods Different concentraction of ethanol, and different exeraction time, differernt solvent quantity were used, with hyperoside, taxifolin and orientin as standards in the orthogonal design method to deploy HPLC to determine content of hyperoside, taxifolin and orientin of each extractive content. The Agilent TC-C18 (4.6 mm×250 mm, 5 μm) was as stationary phase, and acetonitrile-0.1％ phosphoric acid solution (v/v) system was as the mobile phase. The gradient elution, the flow rate was 1.0 ml/min, column temperature was 30 ℃, and detection wavelength was of 290 nm. Results It is the optimum extraction method that 10 times amount of 65％ethanol, extracting 1 hour extraction method optimum extraction process from flowers of Polygonum orientale L. Conclusions The orthogonal design method was feasible of selecting the optimum exeraction process. The components presented at different levels for samples in different areas and at different times.
ABSTRACT
AIM To study the chemical constituents from Polygonum hydropiper L..METHODS The petroleum ether,ethyl acetate and n-butanol fractions of 95％ ethanol extract from P.hydropiper were isolated and purified by silica,Sephadex LH-20 and recrystallization,then the structures of obtained compounds were identified by spectral data.RESULTS Thirteen compounds were isolated and identified as β-sitosterol (1),aniba dimer A (2),succinic acid (3),quercetin (4),gallic acid (5),daucosterol (6),quercetin-3-O-β-D-glucoside (7),kaempferol-3-O-β-D-glucoside (8),quercetin-3-O-β-galactoside (9),kaempferol-3-O-β-galactoside (10),stigmast-4-ene-3β,6a-diol (11),fumaric acid (12),ellagic acid (13).CONCLUSION Compounds 11 and 12 are isolated from genus Polygonum for the first time,compounds 2,8,10 and 13 are first obtained from this plant.
ABSTRACT
Objective: To study and establish the fingerprints of polygonilterba the aerial pevrts of Polygonum hydropiper by HPLC. Methods: The fingerprints of P·hydropiper from different origins were built using Hypersil ODS2 C18 column and acetonitrile-0.2%phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was set at 260 nm. The temperature of column was at 25℃. Total 22 Polygonum Herba samples and adulterants were detected. Results: The mutual mode to HPLC fingerprints was set up, and the 12 common peaks were obtained. The similarities in 15 batches of Polygonum Herba samples were all above 0.95 and those of seven batches of adulterants were below 0.50. Conclusion: The method is simple, stable, and reproducible, which can be uesd to identify Polygonum Herba and its adulterants.