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Objective: To explore the interspecific and intraspecific genetic relationships of six species in Curcuma L. Methods: The superoxide dismutase (SOD), polyphenol oxidase (PPO), and cytochrome-oxidase (COD) were studied by vertical slab Polyacrylamide gel electrophoresis (PAGE). The three isozymes data were analysed by using clustering method UPGMA. Results: The six species in Curcuma L. can largely be distinguished by zymogram of three isozymes; Moreover, PPO and COD can be used to distinguish the different accessions in the same species of Curcuma L. Conclusion: The genetic relationships among different accessions are related with the geographical origin. Clustering approach could make a distinction between Curcuma longa and C. sichuanensis. The differentiation has happened between the wild and cultivated species of Curcuma L.
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Objective: To study the browning mechanism of drying Pinelliae Rhizoma. Methods: The activity of polyphenol oxidase (PPO) was detected by ultraviolet spectrophotometry, and the surface color data of drying Pinelliae Rhizoma were measured by chromaticity instrument. To draw a conclusion that whether Enzymatic browning was the unique factor of browning reaction or not by analyzing the relationship between the above two sets of data. To detect the kinds and content of reducing sugar by HPLC-ELSD and measure the kinds of amino acids in Pinelliae Rhizoma by amino acid analyzer. Through testing the results the possibility of Maillard reaction was analyzed. Results: The factor of the color-change of Pinelliae Rhizoma is not unique, Enzymatic browning is in the prophase of the drying process and Maillard reaction also contributes to the color-change. Conclusion: The factor in the process of Pinelliae Rhizoma color-change is not single. Both enzymatic browning and Maillard reaction impact on the color-change of Pinelliae Rhizoma.
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Objective Bioactive components were extracted from Sargassum fusiforme by using different solvents and their activities were detected in vitro in order to screen potential free radical scavenger.Methods Using the assay system of DPPH,superoxide radical,and in comparison with vitamin C,tea polyphenols and a series of extracts of sea tangle,laver.Results Different solvents had different extraction rates,and extracts by different solvents had different free radical scavenging activities.Conclusion 0.5%Na2CO3 solution had the highest extraction rate and extracts by this solution had the best scavenging ability as well.At the same time,the results obtained by DPPH detection method were more stable than those by the others.
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Objective To study the correlation of polyphenol oxidase(PPO) activity and baicalin synthesis in callus of Scutellaria baicalensisi.Methods PPO Activity was determined by ultraviolet spectrophotometry and the content of baicalin was detected by HPLC.The effects of various substances(ascorbic acid,sodium chloride,benzoic acid,polyvinylpyrrolidone,and copper sulfate) on PPO activity and the content of baicalin were studied.Results There was no baicalin accumulation in the first 20 d of growth period, while PPO activity was expressed slowly during this period.From 20 d to 35 d,PPO activity increased significantly and the baicalin secondary synthesis was restrained.Conclusion The high-level expression of PPO activity do harm the baicalin secondary synthesis.These chemical substances,such as ascorbic acid, sodium chloride,and benzoic acid could inhibit the PPO activity and improve the content of baicalin;It is obvious for ascorbic acid(0.02%) to improve the content of baicalin with 17.6%(82.3 mg/g) compared with the control.However,polyvinylpyrrolidone and copper sulfate could increase the PPO activity and greatly inhibit the baicalin accumulation.