ABSTRACT
In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.
Subject(s)
China , Chromatography, High Pressure Liquid , Geography , Monosaccharides , Chemistry , Polyporus , Chemistry , Polysaccharides , Chemistry , Tandem Mass SpectrometryABSTRACT
Objective: To explore the mechanism of immunomodulatory activity of Polyporus umbellatus polysaccharides (PPS) on murine bone marrow dendritic cells (BMDC). Methods: BMDC phenotype and the function indexes were observed by 3H-TdR incorporation, ELISA, and flow cytometry. Results: Compared with the negative group, PPS could increase the co-expression of CD11c and CD86 molecules on dendritic cells (DC) surface and the production of IL-12 and IL-10 in a dose-dependent manner. PPS also enhanced matured BMDC capacity of T cell initial activation and decreased phagocytosis of BMDC. Anti-Toll-like receptor 4 (TLR4), but not anti-TLR2 or complement receptor 3 (CR3) monoclonal antibodies inhibited PPS-induced production of IL-12 p40 and blocked the combination between fluorescence-labeled PPS (f-PPS) and BMDC. Conclusion: The data demonstrate that PPS could promote the activation of murine BMDC via TLR4 and maturation of immunomodulationy activity.
ABSTRACT
Polyporus Umbellatus polysaccharide(PUP) is a new medicine made by our institute. A model of aged mice was prepared by placing 2-month mice in an ozone circumstance (O_3 conoentration 1. 298 ppm) for 10 days. After the aged mice was fed with PUP for 10 days, the average body weight, body temperature and thymus coefficient can be increased to normal level, superoxide dismutase (SOD) activity of red cells and catalase activity of liver were increased, and liver lipoperoxide (LOP) reduced. It is suggested that PUP may be a good anti-aging agent.