ABSTRACT
Objective: To study the influence on the volatile components of Chinese materia medica (CMM) Moutan Cortex and Cinnamomi Cortex formula granules produced by different preparative processes. Methods: The different preparative processes were compared by determining the content of paeonol in Moutan Cortex formula granule and cinnamaldehyde in Cinnammi Cortex formula granule by HPLC. Results: The contents of paeonol in Moutan Cortex formula granule and cinnamaldehyde in Cinnamomi Cortex formula granule produced by conventional process were significantly lower than those by the other processes, which was carried out as follows: extracting the volatile oil firstly, then drying the herb residue extract, adding the volatile oil by spaying directly or cyclodextrin inclusion finally. Conclusion: The best process of CMM Moutan Cortex and Cinnamomi Cortex formula granules containing volatile components was as follows: extracting the volatile components firstly, then decocting the residues, and the volatile components was enclosed before adding in the granulation.
ABSTRACT
Objective:Vernonia anthelmintica,with complicated components,is a common medicine for the treatment of vitiligo.The purpose of this study was to establish and optimize the separation conditions of high performance liquid chromatography fingerprints(HPLC-FPs) for Vernonia anthelmintica(L.) Willd.Methods: We investigated the HPLC-FPs of different extraction samples by the retention times and relative areas of common peaks in the fingerprints.Results: We established the optimum separation conditions of HPLC-FPs for Vernonia anthelmintica(L.) Willd,and obtained 19 common peaks in the fingerprints.The HPLC-FPs of the 85% ethanol extract were quite similar to those of the semi-bionic extract(SBE) but very different from those of the water extract of Vernonia anthelmintica(L.) Willd.The No.1,4,6,11,12,14 and 16-19 peaks were the endemic ones of the HPLC-FPs of the 85% ethanol extract and SBE of Vernonia anthelmintica(L.) Willd,while No.20-30 were the endemic peaks of the HPLC-FPs of the water extract.Conclusion: The results of our study has provided experimental evidence for further investigation into the constituents absorbed into blood,pharmaceutical technology and pharmacodynamics of Vernonia anthelmintica(L.) Willd.