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Aim To explore the possible mechanism of "component-target-pathway" of Radix Hedysari against target organ damage caused by radiotherapy and chemotherapy, and to verify the " dose-effect" relationship of the main active components. Methods TCMSP, Uniprot, Swiss Target Prediction, GeneCards, Cytoscape, Omicshare and other platforms were used for network pharmacology analysis. Autodock, Pymol and Ligplot were used for molecular docking. The water extract of Radix Hedysari was used for animal experiment verification. The contents of eight main components were determined by HPLC. Results Four active components, eight key targets and four key pathways of Radix Hedysari were identified to resist the damage of target organs caused by radiotherapy and chemotherapy. Molecular docking showed that formononetin and quercetin had good binding activity with HSP90AA1, naringenin and MAPK3, and ursolic acid and TP53. Animal experiments showed that gastrointestinal factors MTL and VIP increased significantly, liver and kidney factors Cr, BUN, AST and ALT decreased significantly, inflammatory factor IL-10 increased significantly and TNF-a decreased significantly. The content of ononm was the highest (2 . 884 8 µg • g "
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AIM: To reveal that the targeted regulation of TGF-β1 by miR-21-5P is the key mechanism that mediates the activation of myocardial fibroblasts, and to clarify the intervention ofRadix Angelica Sinensis and Radix Hedysari ultrafiltration on the mechanism of miR-21-5P targeting to regulate TGF-β1 effect. METHODS: (1) The cells were randomly divided into normal group and irradiation group. The irradiation group received 6Gry single irradiation, and then RT-PCR was used to detect miR-21-5P, and Western Blot was used to detect the expression of α-SMA and TGF-β1. (2) The cells were randomly divided into normal group, irradiation group, miR-21-5P
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The traditional Chinese medicine of Radix Hedysari plays an important role in invigorating gas for as-cending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for Radix Hedysari. This provides a comprehensive and efficient method to improve the quality evaluation of Radix Hedysari, confirming the bioactive components for PBM-enhancement and further develop its medicinal value.
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OBJECTIVE@#To study the intervention effects of Radix Hedysari, Radix Astragalus and compatibility of Angelica Sinensis on blood deficiency model mice induced by cyclophosphamide (CTX).@*METHODS@#The mice were randomly divided into 7 groups, 10 mice each group. The blood deficiency model was established by CTX. The blank group and model group were treated with saline by gavage, while mice in positive group were administered with Lvjiaobuxue granule. Four dosage group were administered with Radix Hedysari, Radix Hedysari-Radix Angelica Sinensis(5:1), Radix Astragalus and Radix Astragalus-Radix Angelica Sinensis(5:1) water decoction. All the drugs were administered to mice for consecutive 7 d. The contents of red blood cell (RBC), lymphocyte(LYM), hematocrit (HCT), white blood cell (WBC), platelet (PLT) were detected by hematology analyzer, while thymus index(TI), spleen index(SI), reticulocyte (RC), marrow karyocyte (MK) were calculated, and the femur by pathological section were observed by microscope.@*RESULTS@#Compared with blank group, the contents of RBC, WBC, HCT, PLT, LYM were decreased in model group (<0.05). Compared with model group, the contents of RBC, WBC, HCT, PLT, LYM, RC and marrow karyocyte were increased in Hedysari-Angelica Sinensis(5:1) and Astragalus Angelica Sinensis(5:1) (<0.05), at the same time, the pathological damage of femur could be improved.@*CONCLUSIONS@#The effect of enrichment blood on blood deficiency model mice in Hedysari-Angelica Sinensis (5:1) and Astragalus-Angelica Sinensis(5:1) were superior to Hedysari and Astragalus.
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Animals , Mice , Angelica sinensis , Astragalus Plant , Cyclophosphamide , Drugs, Chinese Herbal , Plant RootsABSTRACT
An HPLC method was established for the determination of adenosine, γ-aminobutyric acid (GABA) and six flavonoids (calycosin-7-glucoside, ononin, calycosin, isoliquiritigenin, formononetin and medicarpin) in Radix Hedysari. The samples were extracted with methanol by refluxing for 4 h. The HPLCDAD was performed on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile-water as the mobile phase. The column temperature was at 40℃ and the flow rate was 1.0 mL·min-1, while the temperature of drift tube was 110.5℃ and the nebulizing gas flow was 3.1 L·min-1 for the ELSD system. The results showed all the eight components had good linear relationships (r2=0.992 8-1.000 0) in the range of the test concentration. The RSD of precision, stability and repeatability were less than 2%. The average recovery rates were 96.78%-103.45%, and RSD were 0.29%-1.61%. The index component contents of Radix Hedysari form 24 different origins were determined and used as variable factors in clustering analysis. The results were classified into 2 groups basically in accordance with the regional cluster. And the consequence was in consistent with the results of principal component analysis. This HPLC method is simple, shows good sensitive and accurate, and provides the experimental basis for multi-index control of Radix Hedysari. Clustering analysis for Radix Hedysari quality control has a certain reliability and objectivity.
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Objective To observe the effects of Radix Hedysari on collagen area, hyaluronic acid (HA) and laminin (LN) of lung tissues in rat with pulmonary interstitial fibrosis; To investigate the mechanism and screen the best active ingredients in Radix Hedysari.Methods Totally 144 SPF Wistar rats were randomly divided into control group, model group, metacortandracin group, and Radix Hedysari polysaccharide, flavone and saponin groups were set as high-, medium-, and low-dose groups. Pulmonary interstitial fibrosis models were set up by dropping bleomycin into air tube of rats. All groups were taken corresponding medicine with appropriate dose daily on day 2 after models were established for 28 days. Collagen fibrils in lung tissue were observed by Masson dying and contents of HA and LN in lung tissue of rats in each group were detected by radioimmunoassay.Results Compared with the model group, pulmonary alveoli in Radix Hedysari flavone high-, medium-, and low-dose groups and Radix Hedysari polysaccharide low-dose group were relieved obviously; collagenous fiber area in Radix Hedysari flavone low-dose group, Radix Hedysari polysaccharide medium-dose group, and Radix Hedysari saponin low-dose group decreased obviously. Compared with the model group, the content of HA in Radix Hedysari polysaccharide high-dose group, Radix Hedysari flavone high-, medium-, and low-dose groups, and Radix Hedysari saponin high-dose group decreased obviously; the content of LN in Radix Hedysari polysaccharide high-dose group, Radix Hedysari flavone medium- and low-dose groups, and Radix Hedysari saponin medium- and low-dose groups decreased obviously. Conclusion Polysaccharide, flavone and saponin of Radix Hedysari have the abilities to alleviate inflammation of pulmonary alveoli, inhibit proliferation and deposition of collagen fibrils, and reduce the contents of HA and LA in lung tissue. Among these active ingredients, flavone has the best effect.
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Objective:To establish an HPLC method for the determination of calycosin-7-glucoside, genistein, formononetin and medicarpin in Radix Hedysari. Methods: The sample was refluxed with methanol in a water bath. The HPLC was performed on an SunFire C18 column(250 mm × 4. 6 mm,5 μm) with acetonitrile-0. 2% H3 PO4 as the mobile phase by gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was at 35℃. The detection wavelength was 240 nm and the sample size was 20 μl. Results:The linear range of calycosin-7-glucoside was 0.035-1.042 μg(r=0.999 6), that of genistein was 0.027-0.821 μg(r=0. 999 7), that of formononetin was 0. 031-0. 941 μg(r=0. 999 9) and that of medicarpin was 0. 025-0. 745 μg(r=0. 999 6). The average recovery of calycosin-7-glucoside, genistein, formononetin and medicarpin was 100. 32%(RSD=1. 87%), 99. 3%(RSD=1. 76%), 100. 5%(RSD=1. 48%) and 99. 2%(RSD=1. 45%)(n=6), respectively. Conclusion:The method shows short analyt-ic time, good stability and promising operation accuracy, which provides the reference for the quality control of Radix Hedysari.
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Objective To discuss radix hedysari flavonoids mechanism of preventing pulmonary fibrosis.Methods Totally 216 SPF Wistar rats were randomized into normal group, model group, prednisone group, radix hedysari flavonoids high, medium, and low dose groups, and then pulmonary fibrosis model was established by intratracheal dripping of bleomycin. From the second day after modeling, every treatment group received gavage with the corresponding dose of medicine at the planned time for 7, 14, and 28 continuous days. Expressions of MMP-2 and TIMP-1 protein were detected by immunohistochemical method.Results When radix hedysari flavonoids high dose group was at the 7th, 14th, 28th day, and medium dose group was at the 14th day, MMP-2 protein expression was lower than model group, similar to prednisone group. When radix hedysari flavonoids high dose group was at the 14th, 28th day and medium dose group was at 14th day, TIMP-1 protein expression was lower than model group.Conclusion Radix hedysari flavonoids can adjust the expressions of MMP-2 and TIMP-1 protein at different phases, and tend to make the balance of MMPs/TIMPs, which may be an effective mechanism for the inhibition of fibrosis process.
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Objective To discuss the effects of Radix Hedysari flavonoids on microvessel density (MVD) of pulmonary interstitial fibrosis model rats, and provide evidence for its development. Methods SPF Wistar rats were divided into blank group, model group, prednisone group, and Radix Hedysari flavonoids high-, medium- and low-dosage group. Pulmonary interstitial fibrosis model was established by intratracheal instillation of bleomycin. From the second day after model established, each drug treatment group was administered with corresponding drugs intragastrically at different points in time for 14 and 28 days. MVD was detected by immunohistochemistry. Results Neomicrovessel was increased significantly in pulmonary tissue of model rats of 14 days treatment, but slightly decreased in 28 days. MVD in 14, 28 days model group was significantly more than blank group (P<0.01). MVD in 14, 28 days Radix Hedysari flavonoids high dosage group was significantly less than model group (P<0.01). MVD in Radix Hedysari flavonoids medium-dosage group was between high-and low-dosage group, and MVD in medium-dosage group was similar with that in model group. Conclusion Radix Hedysari flavonoids could inhibit the formation of neomicrovessel in a dose dependent manner.
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OBJECTIVE: To isolate and purify radix hedysari polysaccharide 3 (HPS-3) and determine the monosaccharide composition and the contents of polysaccharide in its four components. METHODS: Radix Hedysari polysaccharide was extracted by water, precipitated with ethanol, treated with Sevag method, decolored with H2O2, then precipitated with ethanol and dialyzed. By using DEAE-cellulose 52 and Sephadex G-100, HPS-3-A, HPS-3-B, HPS-3-C and HPS-3-D were purified. Their purity were determined by high performance gel permeation chromatography (HPGPC). By TLC and GC, the monosaccharide composition of four components was analyzed, and then the modified phenol sulfuric acid method was used to determine the content of polysaccharide of the four fractions. RESULTS: HPS-3-A was composed mainly of glucose, and the content of polysaccharide was 99.2%. But HPS-3-B, HPS-3-C and HPS-3-D were all composed mainly of arabinose and galactose. The contents of polysaccharide of them were 96.5%, 95.3% and 95.2%, respectively. CONCLUSION: This study provides the foundation for the research of structure-function relationship of Radix Hedysari polysaccharide 3. Copyright 2012 by the Chinese Pharmaceutical Association.
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OBJECTIVE: To study the quality standard of Radix Hedysari from Gansu province. METHODS: Radix Hedysari was analyzed in respects of its origin, property, microscopic characteristics, physicochemical property, TLC identification, moisture, determination of ash and determination of ethanol-soluble extractives etc. RESULTS: The results showed that the Radix Hedysari from Guansu was of high quality and in which, few counterfeit products was noted. CONCLUSION: In this study, a systematic evaluation for the Radix Hedysari from Gansu province was achieved, and it provides a scientific evidence for the quality control Radix Hedysari from Gansu province and guarantee of its genuine property.
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AIM: To establish a method for determing polysaccaride content of Radix Hedysari(Hedysarum Polybotrys Hand-Mazz). METHODS: To determine content of Radix Hedysari polysaccaride by HPLC and ELSD. RESULTS: The composes of Radix Hedysari polysaccaride Ⅰ、Ⅱ、Ⅲ、Ⅳ are all Rhamnose、Arabinose、Xylose、Glucose、Galactose,the average content of polysaccharides is 25.34%、24.23%、15.08%、17.24%. CONCLUSION: The contents of Radix Hedysari polysaccaride in Radix Hedysari can be determined by HPLC.
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The effects of RHPS & APS on immune function in normal mice and immunosuppressed mice were studied. The results showed that RHPS and APS enhanced not only the phagocytosis of peritoneal mac-rophages in normal mice, but also antagonized completely the immun- Osuppresslve effect of prednisoione. RHPS and APS increased both the lymphocyte transformation rate induced by PHA and ANAE+ rate in normal mice, and antagonized also the suppressive effects of GY or PDS. No significant influence was found on the proliferation of rabbit lymphocyter treated with PHA in vitro. It seems that no obvious difference of effect was found on immune function between RHPS and APS.