ABSTRACT
@#ObjectiveTo establish a method of isolation and culture of neural stem cells(NSCs). MethodsTissues of ventral midbrain were isolated from a rat embryo,and the NSCs were cultured stimulated with basic fibroblast growth factor(bFGF)in vitro.The cells were identified by immunocytochemistry.ResultsNSCs proliferated into neurosphere in symmetric and non-symmetric cleavage ways,and differentiated into neuron, astroglia and oligodendrosyte. ConclusionsThe method has been established to isolate and culture the neural stem cells.
ABSTRACT
Objective To establish a model of injury to primarily cultured spinal cord neurons,mimicking the neuronal injury after complete transactional spinal cord trauma,for the sake of exploring changes in expression of an immediately-early gene,c-jun,in central nervous system injury. Methods Spinal cords were removed form fetal Wistar rats at the 14th gestation day and the neurons were cultured for 10 to 12 days.Then,mechanical injury was applied to the neurons by making regular scores on the culture disk under direct vision with the aid of a self-made standard template.Morphology of the injured neurons and changes in expression of c-Jun protein were observed before and at different intervals after injury. Results c-Jun expression was noted in neuronal nuclei 10 min after injury and its peak appeared at 2 hrs.Besides,the density of positive neurons bore evidently an inverse proportion with their distance from the scores.Conclusion\ Positive expression in injury neurons show that c-jun gene enters the nucleoli of injured neurons and takes the role of “the third messenger” at early time after neuronal injury.
ABSTRACT
In a conventional embryology laboratory, standard serial sections of the transverse section of 10 mm. pig embryo were used as models to study heart development as 10 mm. pig embryo can depict all details necessary for such study. Due to the difficulty in obtaining pig embryo for slide preparation, the author tried to study such development with mammals wich were easily available. Rats which can be bred in lab or satory to obtain any desired serial sections were also studied. The results of the study of serials of 6 mm. rat embryo at the heart level showed that all detalis necessary can be discernible. In addition, there were a less number of sections to cover the heart area proving that pig embryo can be substituted by rat embryo in embryology studies.