Notas psicanalíticas acerca das arquiteturas do infamiliar na literatura fantástica / Psychoanalytic notes on the architectures of the uncanny in fantastic literature

Defende-se que a literatura fantástica constitui um material valioso de pesquisa para o psicanalista, haja vista que ela está comprometida em evocar no leitor o sentimento do infamiliar (Unheimliche), entendido como a manifestação episódica de um conteúdo atávico do psiquismo que se encontrava até então em estado latente, acarretando a regressão ao narcisismo primário e a dissolução temporária e localizada das fronteiras do Eu. Propõe-se a expressão arquiteturas do infamiliar para designar a conjuntura estético-literária que caracteriza esse projeto narrativo. Para evidenciar a especificidade dos recursos textuais que favorecem esse arranjo, sua sintaxe é contraposta à dos chistes. Então, por meio da referência às concepções de anamorfose, leitor implícito, fantasia e construção, busca-se esclarecer os mecanismos psíquicos relacionados à leitura e à criação dos textos fantásticos, sublinhando o caráter volátil e mutante de suas temáticas e estratégias e a sua íntima relação com a cultura e o social. Pontua-se que o estudo desses artifícios literários podem trazer contribuições significativas para o entendimento das vicissitudes da fantasia e da delimitação dos limites do Eu e da realidade. Ao final, comenta-se o conto O homem de areia, de E. T. A. Hoffmann, à luz da proposta apresentada.

This paper defends that fantastic literature constitutes a valuable research material for the psychoanalyst, given that this literary modality is committed to evoking in the reader the feeling of the uncanny (Unheimliche), understood as an episodic manifestation of an atavistic in latent state psychic content, causing the regression to primary narcissism and a temporary and localized dissolution of the borders of the Self. The expression architectures of the uncanny is proposed to designate the aesthetic-literary conjuncture that characterizes this project. To highlight the specificity of the textual resources that favor this arrangement, its syntax is contrasted with that of jokes. Through the reference to the concepts of anamorphosis, implicit reader, fantasy and construction, one seeks to clarify the psychic mechanisms related to the reading and creation of fantastic texts, underlining the volatile and mutating character of their themes and strategies and their intimate relationship with culture and society. One argues that the study of these literary devices can bring significant contributions to the understanding of the delimitation of Self and reality, and the vicissitudes of fantasy as well. At the end, the short story The Sandman, by E. T. A. Hoffmann, is commented in the light of the this proposal.

Impact of different storage conditions on nutrient composition of human milk at different stages / 上海预防医学

Objective To investigate the changes of nutrients in colostrum， transitional milk， mature milk and late milk after storage at indoor temperature for 3 h， at 4 ℃ for 24 h， at -18 ℃ for 30 d， and at -18 ℃ for 90 d， so as to select the most suitable storage method of milk. Methods Fifteen pregnant women registered in the Shanghai Hongkou Liangcheng Village Street Community Health Service Center， who were expected to give birth from January to June in 2019， were selected. The breast milk of each woman was followed from delivery to 10 months after breast-feeding. Colostrum， transitional milk， mature milk and late milk were sampled， and each sample was divided into 5 parts. Changes in protein， fat， lactose， minerals， carbohydrates， vitamins and bacterial colony number of breast milk were detected according to 0 h（16-18 ℃）， 3 h （16-18 ℃）， 24 h （4 ℃） to 37 ℃， 30 d （-18 ℃） to 37 ℃ after thawing at 40 ℃ and 90 d （-18 ℃） to 37 ℃ after thawing at 40 ℃. Results The results showed that the nutritional components of milk in different stages decreased with time. However， there was no significant difference in the decline of nutritional components in different stages of milk after the same duration of time （ P >0.05）. Conclusion The milk at each stage can be stored， and the lactating mother can store the excess milk at any stage as she wishes.

Impact of different storage conditions on nutrient composition of human milk at different stages / 上海预防医学

Objective To investigate the changes of nutrients in colostrum， transitional milk， mature milk and late milk after storage at indoor temperature for 3 h， at 4 ℃ for 24 h， at -18 ℃ for 30 d， and at -18 ℃ for 90 d， so as to select the most suitable storage method of milk. Methods Fifteen pregnant women registered in the Shanghai Hongkou Liangcheng Village Street Community Health Service Center， who were expected to give birth from January to June in 2019， were selected. The breast milk of each woman was followed from delivery to 10 months after breast-feeding. Colostrum， transitional milk， mature milk and late milk were sampled， and each sample was divided into 5 parts. Changes in protein， fat， lactose， minerals， carbohydrates， vitamins and bacterial colony number of breast milk were detected according to 0 h（16-18 ℃）， 3 h （16-18 ℃）， 24 h （4 ℃） to 37 ℃， 30 d （-18 ℃） to 37 ℃ after thawing at 40 ℃ and 90 d （-18 ℃） to 37 ℃ after thawing at 40 ℃. Results The results showed that the nutritional components of milk in different stages decreased with time. However， there was no significant difference in the decline of nutritional components in different stages of milk after the same duration of time （ P >0.05）. Conclusion The milk at each stage can be stored， and the lactating mother can store the excess milk at any stage as she wishes.

Prática de biblioterapia no Brasil e no exterior: principais experiências com a terapia pela leitura / Bibliotherapy practice in Brazil and abroad: some experiences with reading therapy / Práctica de la biblioterapia en Brasil y en el extranjero: algunas experiencias con la terapia de lectura

O presente estudo tem como objetivo conhecer as principais experiências com a biblioterapia voltadas para pessoas acometidas por doenças variadas, sejam físicas e/ou mentais, ou sociais no Brasil e no exterior. Justifica-se por apresentar a função terapêutica da leitura. Sua base são os conceitos teóricos e históricos da biblioterapia, o conhecimento das principais experiências com a biblioterapia no Brasil e um comparativo entre a realidade nacional e internacional da sua utilização. Para tanto, foi feita uma pesquisa exploratória, qualitativa e bibliográfica em bases de dados nacionais e internacional (LISA) com material publicado sobre experiências que utilizassem a biblioterapia para o bem-estar do indivíduo. Desse modo, observa-se que o Brasil caminha para ampliação da utilização da terapia, enquanto outros países a utilizam nos âmbitos hospitalar, escolar, prisional, entre outros. Isso permite concluir que a biblioterapia proporciona saúde e bem-estar aos que dela fazem uso.

This study aims to find out the main experiences with bibliotherapy aimed at people affected by various diseases, whether physical and/or mental, or social in Brazil and abroad. It is justified by presenting the therapeutic function of reading. This study is based on the theoretical and historical concepts of bibliotherapy, the knowledge of the main experiences with bibliotherapy in Brazil and comparing it with the national and international reality regarding its use. To this end, it is an exploratory, qualitative and bibliographic research, which searched for published material on national and international databases (LISA) on experiences that used bibliotherapy for the well-being of the individual. Thus, it is observed that Brazil is moving towards expanding the use of therapy, while other countries use it in hospitals, schools, prisons, among others. This allows us to conclude that bibliotherapy provides health and well-being to those who use it.

Este estudio tiene como objetivo conocer las principales experiencias con biblioterapia dirigidas a personas afectadas por diversas enfermedades, físicas y/o mentales, o sociales en Brasil y en el exterior. Se justifica presentando la función terapéutica de la lectura. Este estudio se basa en los conceptos teóricos e históricos de la biblioterapia, el conocimiento de las principales experiencias con biblioterapia en Brasil y su comparación con la realidad nacional e internacional en cuanto al uso de esta terapia. Para ello, se trata de una investigación exploratoria, cualitativa y bibliográfica, que buscó material publicado en bases de datos nacionales e internacionales (LISA) sobre experiencias que utilizaron la biblioterapia para el bienestar del individuo. Así, se observa que Brasil avanza hacia la expansión del uso de la terapia, mientras que otros países la utilizan en diversos nichos, como hospital, escuela, prisión, entre otros. Esto nos permite concluir que la biblioterapia brinda salud y bienestar a quienes la utilizan.

Conceptualización de la Hoja de vida de lector como instrumento de evaluación formativa en la educación superior / Conceptualization of the reader curriculum vitae as a tool of formative assessment in higher education / Conceptualização do Currículo do Leitor como um instrumento de avaliação formativa no ensino superior

RESUMEN El objetivo de este artículo de investigación es conceptualizar la Hoja de vida de lector como instrumento de evaluación formativa al inicio de la Educación Superior: Con el fin lograr tal propósito, el Grupo de Investigación Cultura y Narración en Colombia (Cuynaco) se basa en el paradigma hermenéutico-interpretativo de las Ciencias Sociales y en el método documental de investigación con carácter proyectivo. Por tanto, se realiza, en primer lugar una revisión de la literatura en torno a instrumentos de valoración metacognitiva; por la escasez de investigaciones al respecto, se propone una aproximación al análisis bibliométrico del tema y un diálogo entre los instrumentos de evaluación hallados. En segundo lugar se expone una aproximación teórica alrededor de conceptos tales como Hoja de vida, Lector y Evaluación formativa A partir de lo anterior, surge la definición de Hoja de vida de lector como un instrumento metacognitivo con características formativas particulares en comparación con los existentes.

ABSTRACT The aim of this research article is to conceptualize the Reader Curriculum Vitae as a formative assessment tool for at the beginning of University. In order to achieve this purpose, the Research group Cuynaco bases its study on the Social Science hermeneutical approach and a documentary and projective review. Therefore, first of all, the research group conducts a literature review around metacognitive assessment tools. Due to a few studies, the group proposes an approach to the bibliometric analysis of the topic and a discussion among the found assessment tools. Second, it sets out a theoretical approach around concepts such as Curriculum Vitae, Reader and Formative Assessment. Based on the foregoing, it presents the definition of Reader Curriculum Vitae, as a metacognitive tool with particular formative characteristics, compared with the existing ones.

RESUMO O objectivo deste artigo de investigação é conceptualizar o Currículo do Leitor como um instrumento de avaliação formativa no início do Ensino Superior Para alcançar tal objectivo, o Grupo de Investigação Cultura e Narração na Colômbia (Cuynaco) baseia-se no paradigma hermenêutico-interpretativo das Ciências Sociais e no método documental de investigação com carácter projectivo. Assim, em primeiro lugar é feita uma revisão da literatura sobre instrumentos de avaliação metacognitiva; devido à escassez de investigação sobre o tema, propõe-se uma abordagem à análise bibliométrica do tema e um diálogo entre os instrumentos de avaliação encontrados. Em segundo lugar, é apresentada uma abordagem teórica em torno de conceitos tais como Folha de Vida, Leitor e Avaliação Formativa. Do acima exposto, emerge a definição da Folha de Vida do Leitor como um instrumento metacognitivo com características formativas particulares em comparação com os existentes.

A novel role of tumor suppressor ZMYND8 in inducing differentiation of breast cancer cells through its dual-histone binding function

Accumulating evidences indicate the involvement of epigenetic deregulations in cancer. While some epigeneticregulators with aberrant functions in cancer are targeted for improving therapeutic outcome in patients,reinstating the functions of tumor-suppressor-like epigenetic regulators might further potentiate anti-cancertherapies. Epigenetic reader zinc-finger MYND-type-containing 8 (ZMYND8) has been found to be endowedwith multiple anti-cancer functions like inhibition of tumor cell migration and proliferation. Here, we reportanother novel tumor suppressor role of ZMYND8 as an inducer of differentiation in breast cancer cells, byupregulating differentiation genes. Interestingly, we also demonstrated that ZMYND8 mediates all its antitumor roles through a common dual-histone mark binding to H4K16Ac and H3K36Me2. We validated thesefindings by both biochemical and biophysical analyses. Furthermore, we also confirmed the differentiationinducing potential of ZMYND8 in vivo, using 4T1 murine breast cancer model in Balb/c mice. Differentiationtherapy holds great promise in cancer therapy, since it is non-toxic and makes the cancer cells therapysensitive. In this scenario, we propose epigenetic reader ZMYND8 as a potential therapeutic candidate fordifferentiation therapy in breast cancer

Microscopio como Lector de Absorbancia con Utilidad en Análisis Clínicos / Microscope as Absorbance Reader with Utility in Clinical Analysis

Resumen La descentralización de laboratorios clínicos, se encuentra en desarrollo, lo anterior ha llevado a diseñar instrumentos que ofrecen resultados rápidos, confiables y al lado del paciente, esta tendencia se le conoce como prueba en el punto de atención (point of core testing POCT) y brinda la posibilidad de dar un seguimiento inmediato al padecimiento. El objetivo de esta investigación fue la implementación de un medidor de absorbancia, empleando la estructura de un microscopio óptico, al cual se le ha adaptado un filtro de luz, y una cámara digital. Lo anterior permite obtener valores de intensidad promedio de imágenes sólidas microscópicas de reacciones enzimáticas, y a partir de ellas estimar absorbancia o concentración. Como resultados se presentan rectas de calibración de absorbancia y mediciones de concentraciones para los casos de violeta de genciana, un kit de glucosa oxidasa y muestras problemas de pacientes voluntarios. Concluimos que existe un error de medición menor de ±1mg/dL comparados con las mediciones de un lector de placas de Elisa y un analizador de química sanguínea semiautomatizado. Teniendo en cuenta lo anterior el sistema resulta ser una alternativa viable para la estimación de absorbancia y aumenta la funcionalidad de microscopios ópticos en clínicas de salud.

Abstract The decentralization of clinical laboratories is under development, which has led to the design of instruments that offer fast, reliable and patient-side results, this trend is known as point of core testing (POCT) and It offers the possibility of giving an immediate follow-up to the disease. The objective of this investigation was the implementation of an absorbance meter, using the structure of an optical microscope, to which a light filter and a digital camera have been adapted. This allows to obtain values of average intensity of solid images of enzymatic reactions, and from them to estimate absorbance or concentration. As results, we present absorbance calibration lines and concentration measurements for cases of gentian violet, a glucose oxidase kit and samples of volunteer patients. We conclude that there is a measurement error of less than ± 1mg / dl compared with the measurements of an Elisa plate reader and a semi-automated blood chemistry analyzer. Taking into account the above, the system turns out to be a viable alternative for estimating absorbance and increasing the functionality of optical microscopes in health clinics.

Effect of different types of microplate and loading volumes on the detection results of multi-function microplate reader / 中国职业医学

OBJECTIVE: To evaluate the effect of different types of microplate and loading volumes on the detection results of multi-function microplate reader, and to optimize the analysis method. METHODS: A multi-function microplate reader was used to perform spectrum scanning on each of 5 detection holes of common and ultraviolet(UV) microplates, and the applicable detection wavelength range was those with light transmittance greater than 80.00%. The optical density measurement was carried out on each 12 detection holes of common and UV microplates at different wavelengths, then the matching of the detection holes was compared. Potassium permanganate was quantitatively analyzed by common microplate and UV microplate, while acetone was analyzed by UV microplate, and then detection limit, lower limit of quantitation(LLQ), accuracy and precision at different loading volumes and concentrations were calculated and compared. RESULTS: The shortest applicable analyzing wavelengths for common and UV microplates were(362±2) and(230±3) nm respectively, while the longest applicable analyzing wavelengths were both 1 000 nm. The light transmittance of UV microplate was higher than that of common microplate when the analyzing wavelengths were lower than 400 nm(P<0.01). The deviation and range of light transmittance of detection holes analyzed by UV microplates were smaller than that of common microplates when the analyzing wavelengths were 350-1 000 nm(P<0.05). The detection limit and LLQ of potassium permanganate by multi-function microplate reader was not associated with the types of microplate. The adding standard recoveries of potassium permanganate by UV microplate was higher than that by common microplate(P<0.05). The adding standard recoveries of potassium permanganate by loading volumes of 200 and 250 μL was lower than that by loading volumes of 150 μL(P<0.01), while adding standard recovery of acetone by loading volumes of 200 μL was lower than that by loading volumes of 150 μL(P<0.05). CONCLUSION: When using a multi-function microplate reader to detect chemicals, it is recommended to use UV microplate with wavelengths at the range of 230-1 000 nm, and loading volumes of 200-250 μL.

Structural Insights into N-methyladenosine (mA) Modification in the Transcriptome / 基因组蛋白质组与生物信息学报·英文版

More than 100 types of chemical modifications in RNA have been well documented. Recently, several modifications, such as N-methyladenosine (mA), have been detected in mRNA, opening the window into the realm of epitranscriptomics. The mA modification is the most abundant modification in mRNA and non-coding RNA (ncRNA). At the molecular level, mA affects almost all aspects of mRNA metabolism, including splicing, translation, and stability, as well as microRNA (miRNA) maturation, playing essential roles in a range of cellular processes. The mA modification is regulated by three classes of proteins generally referred to as the "writer" (adenosine methyltransferase), "eraser" (mA demethylating enzyme), and "reader" (mA-binding protein). The mA modification is reversibly installed and removed by writers and erasers, respectively. Readers, which are members of the YT521-B homology (YTH) family proteins, selectively bind to RNA and affect its fate in an mA-dependent manner. In this review, we summarize the structures of the functional proteins that modulate the mA modification, and provide our insights into the mA-mediated gene regulation.

Freud e seus leitores / Freud and his readers

O presente trabalho procura descrever a relação que Freud estabelece com a figura do leitor em suas Conferências introdutórias à psicanálise e os desafios que esta relação apresenta para a tradução deste texto. As tensões e conflitos presentes neste contato são um prolongamento do conflito psíquico e da dinâmica inconscientes que Freud deseja apresentar a estes leitores. Em seu esforço didático para explicar o núcleo da teoria psicanalítica a partir da investigação de lapsos, sonhos e neuroses, ele elabora sucessivas analogias que também ilustram, por vias indiretas, a natureza do inconsciente psíquico.

This paper attempts at describing the connection between Freud and the reader in his Introductory Lectures on Psychoanalysis, as well as discussing some of the issues brought by this relation to the work of translating the text into Portuguese. The tensions and conflicts arising from this contact between the author and his audience could be understood as an extension of the psychical conflict and the dynamics of the unconscious Freud is bringing forth to the readers. His attempt to explain the core of psychoanalytical theory from his investigations on lapses, dreams and neurotic symptoms will ultimately create analogies which may also reveal something about the nature of the psychical unconscious.

Variety methods study on hemolytic effect of colored injection doxorubicin hydrochloride in vitro / 药物评价研究

Objective The visual inspection method were not appropriate to perform a hemolysis evaluation for colored injection like doxorubicin hydrochloride,this article adopted three methods to evaluate the hemolysis test of doxorubicin hydrochloride in vitro and provide reference for clinical drug safety.Methods Using rabbit erythrocytes as experimental object,the durg concentration 4.0 and 2.0 mg/mL was chosen which range of clinical concentration and preclinical safety evaluation concentration,to evaluate the hemolysis test of doxorubicin hydrochloride injection with blood analyzer test,direct colorimetric assay,and indirect colorimetric assay.Results The evaluation results of three different methods were very consistent.The tube's hemolysis rate of 4.0 mg/mL dose was far greater than 5％,which means serious hemolysis;Only 0.1 mL tube of 2.0 mg/mL dose (according to the drug concentration equal to 0.5 mL tube of 0.4 mg/mL drug concentration) without hemolysis occurring,the other tubes' hemolysis rates were far greater than 5％,which means serious hemolysis.Conclusion The hemolysis phenomenon may occur when 2.0 mg/mL dose of doxorubicin hydrochloride solution for iv injection is used in clinic and dilution (final concentration not more than 0.4 mg/mL) is recommended.

Practice of Space Renovation for Medical Libraries / 医学信息学杂志

Taking the physical space renovation of the library of Dalian Medical University as an example,the paper states the significance of space renovation for libraries,detailedly introduces the physical space renovation plan including co-learning space,medical humanistic reading space,overseas students culture reading space,culture presentation space,etc.,and provides references for the library space renovation of other medical colleges.

Characteristics of Internet users' information behaviors and strategies for library service in medical colleges and universities / 中华医学图书情报杂志

Objective To provide the evidence for working out the strategies for library service by investigating the characteristics of Internet users' information behaviors in medical college and university library. Methods The In-ternet users' information behaviors in electronic reading room of medical college and university library were recorded using management software and analyzed using the SPSS. Results Medical undergraduates were the major readers in electronic reading room of medical college and university library. Their Internet information behaviors were charac-terized by information searching which accounted for 55. 19%. The use of foreign databases, especially PubMed, was more than that of domestic databases. The time of readers to search and use information was centralized in their working hours. Conclusion Library should help its readers to know how to search the databases, how to make full use of Internet resources, and how to improve their learning efficiency in order to make sure that the readers can ef-fectively search and use the Internet information.

RDAWA-based reader-driven acquisition of library holdings / 中华医学图书情报杂志

A collective intelligent perception-supported and RDAWA-based reader-driven acquisition model of li-brary holdings was proposed with the development of academic library holdings as its background .The design ideas of RDAWA were described , the master framework of RDAWA was analyzed , the considerations in design of RDAWA that simplified the input procedure and met the reading requirements of readers were elaborated with the biblio-graphic information database , data analysis module and acquisition order generation algorithm summarized .

Problems in foreign language books recommended by readers and suggestions for their solution / 中华医学图书情报杂志

Foreign language books recommended by readers include those marked with a circle by expert committee , pushed by E-mail, recommended on Internet , marked with a circle in booksellers catalogues , recommended on agentwebsite platforms, recommended on the spot book fair, selected according to bibliometric analysis and readers suggestions .Following suggestions were put forward for the solution of problems in foreign language books recommended by readers, such as immediate feedback, improving the acquisition ability of librarians and their website techniques, strengthening participation of subject librarians, and maintaining the sustainability of reader recommendations .

A summary of service for persons with dyslexia in libraries of USA / 中华医学图书情报杂志

Described in this paper are the definition of dyslexia, service for persons with dyslexia in domestic and foreign libraries, and the practices in relation with the service for persons with dyslexia in libraries of USA, such as law support, service provided by US Congress Library, and Bookshare service, in an attempt to provide reference for such service in domestic libraries.

The existence way for hospital library with the aid of library colleague advantages / 中华医学图书情报杂志

After the difficult position of hospital library and the experiences of Shanghai Public Health Clinical Center Library in meeting clinical and medical needs were described, such as relying on the advantages of Fudan library col-league, improving professional literacy, providing subject service, and expanding service contents, the author pointed out that providing excellent service through multiple channels and ways is the key for hospital library to improve its value and position and its sustainable development .

Reader association participation-based crowd sourcing of virtual reference service in medical college and university libraries / 中华医学图书情报杂志

As a brand new service mode on Internet,crowd sourcing can solve the problems that need high cost and professionals by drawing on the wisdom of tens of thousands of Internet users. Medical college and university libraries should thus strengthen their virtual reference service teams and further improve their virtual reference service level by introducing the crowd sourcing concept,depending on the support of reader association and drawing on the wisdom of readers.

Ideas for introducing accumulative point system into hospital library / 中华医学图书情报杂志

After a description of the concept of accumulative point system and studies on its theories and practice in libraries, it was pointed out that introduction of accumulative point system into hospital library could improve the utilization of resources, normalize the borrowing behaviors of readers, creating hormonal and fair borrowing environ-ments, and certain measures were put forward for implementing accumulative point system in hospital library.

Role of media guidance in popularization of reading ancient books on traditional Chinese medicine and its enlightenments / 中华医学图书情报杂志

The popularization of reading ancient books on traditional Chinese medicine has been in abasinstate due to their contents , their readers and the reading competence of their readers .The role of media guidance in popu-larization of reading ancient books on traditional Chinese medicine was thus elaborated with the exhibition of ancient books on traditional Chinese medicine in Library of Nanjing University of Traditional Chinese Medicine as an exam-ple, which may provide certain reference for changing the ideas of libraries and popularizing the reading of charac-teristic documents .