Mechanism of Genetic Robustness / 中国生物化学与分子生物学报

Genetic robustness refers to the fact that an organism has a buffer system that can maintain normal development, even if the existence of genetic mutations during biological development. Previous research on the underlying mechanism of genetic robustness mainly involves in genetic redundancy and distributed robustness, both of which are triggered at the protein level. Recently, a new genetic compensation response (GCR) mechanism has been discovered in zebrafish, which occurs in knockout rather than knockdown individuals and is triggered upstream of protein feedback regulation. Since there are many concepts related to genetic robustness, this review attempts to clarify these concepts from the types of compensation genes and triggering modes. Additionally, we aim to understand the latest discovered GCR mechanism and look forward to studying the function of specific genes based on functional compensation mechanisms.

Analysis of operation efficiency and resource allocation of clinical departments in a hospital based on data envelopment andlysis / 中华医院管理杂志

Objective:To establish a calculation model for the operational efficiency and resource allocation of clinical departments in hospitals, for references for hospitals to optimize resource allocation.Methods:The informations including hospitalization time, nursing grade, etc. of inpatients admitted by 32 clinical departments in a tertiary public hospital from January to December in 2021 were extracted. A data envelopment analysis method was conducted on the operation efficiency and input edundancy of the departments. The K-means algorithm was used to divide inpatients into 3 categories according to the level of medical workload. Taking the numbers of doctors, nurses and beds as the input indicators, and the numbers of patients in the 3 categories as the output indicators, a BCC model 1 was established to evaluate the efficiency of resources invested by clinical departments into professional human value. At the same time, a BCC model 2 was established with the total number of patients admitted and medical income as the output indicators to evaluate the efficiency of resources invested by clinical departments into economic benefits.Results:A total of 38 147 inpatients were enrolled. There were 14 departments with overall technical efficiency (OTE) =1.000 in the BCC model 1, 10 departments with OTE=1.000 in the BCC model 2, and 8 departments with OTE=1.000 in the 2 models. As for the input redundancy, 6 departments had high input redundancy in the BCC model 1, 11 departments had high input redundancy in the BCC model 2, and 4 departments had high input redundancy in both models.Conclusions:The model established by this study could effectively evaluate the operational efficiency and input redundancy of clinical departments, identify departments with high workload and low economic benefits, and provide reference for the rational allocation of medical resources in hospitals.

Dinámica funcional de la comunidad bacteriana del lodo activado de una planta de tratamiento de agua residual y su relación con variables ambientales y de operación / Funcional dynamics of the bacterial community from activated sludge wastewater treatment plant and its relationship with environmental and operational variables

RESUMEN El proceso de lodos activados es comúnmente utilizado en plantas de tratamiento de aguas residuales (PTAR) para reducir la concentración de materia orgánica disuelta que llega en el afluente. A pesar de los avances en el estudio de las comunidades microbianas de los lodos activados, falta mucho para entender su potencial funcional y su variabilidad ante las fluctuaciones temporales del afluente y los cambios en la operación de las PTAR. En consecuencia, en este trabajo se analizó la variación del potencial metabólico de la comunidad bacteriana del lodo activado a lo largo de un ciclo anual y se relacionó esa dinámica con variables ambientales y operacionales de una PTAR con alcantarillado combinado. La predicción del metagenoma de la comunidad bacteriana se realizó con PICRUSt2. Esta aproximación permitió evidenciar el fenómeno de redundancia funcional en la comunidad. También, se logró analizar la fluctuación temporal de los genes asociados a procesos relacionados con los ciclos del nitrógeno y azufre y su relación con variables ambientales y operacionales de la PTAR. Se encontró que dichos procesos están relacionados diferencialmente con la precipitación y los cambios en la edad de lodo observados para el periodo estudiado. Estos resultados contribuyen al entendimiento de las dinámicas de la comunidad bacteriana con relación al funcionamiento de este tipo de sistemas biotecnológicos.

ABSTRACT Activated sludge process is commonly used in wastewater treatment plants (WWTP) where a microbial community removes the organic matter from the influent. Despite the advances in the study of this community, there is still a gap of knowledge about its functional potential and its variability due to temporary fluctuations of the influent and the WWTP operation. Therefore, this work analyzed the metabolic potential variation of the activated sludge bacterial community throughout an annual cycle. Furthermore, the dynamics of the bacterial community was related to environmental and operating variables of a WWTP with combined sewerage. The metagenome prediction of the bacterial community was carried out with PICRUSt2. This approach allowed to demonstrate the phenomenon of functional redundancy in the community. Moreover, the temporal fluctuation of genes associated with the nitrogen and sulfur cycles and their relationship with environmental and the operating variables of the WWTP were analyzed. It was found that those processes were differentially related to precipitation events and variations in the sludge age observed during the studied period. These results contribute to the understanding of the bacterial community dynamics in relation to the functioning of this type of biotechnological systems.

Application of fetal atrial septal excursion index and comprehensive Z-scores of cardiovascular structures in fetuses with redundancy foramen ovale flap / 中华超声影像学杂志

Objective:To investigate the application of fetal atria septal excursion index (ASEI) combining cardiovascular structure Z-scores in fetuses with redundancy foramen ovale flap (RFOF).Methods:Twenty-two fetuses with RFOF diagnosed by fetal echocardiography in Sir Run Run Shaw Hospital from December 2019 to September 2021 were selected as the RFOF group and 62 normal fetuses as the control group. The movement of the foramen ovale valve was observed in the four chamber view, and the maximum distance of the foramen ovale valve bulging, the total length of the atrial septum, the foramen ovale diameter, the diameters of the aortic valve, the pulmonary valve, the ascending aorta, the aortic isthmus, the descending aorta and the left and right ventricular areas were measured. ASEI, foramen ovale diameter, foramen ovale valve excursion distance, the ratio of foramen ovale diameter to total atrial septum length, aortic valvular annular diameter Z-score(AOV Z-score), pulmonary valvular annular diameter Z-score(PV Z-score), ascending aorta diameter Z-score(AOA Z-score), ascending aorta diameter Z-score (AOA Z-score), aortic isthmus Z-score (AOI Z-score), descending aorta diameter Z-score (AOD Z-score), diastolic right ventricular area Z-score (RV area Z-score) and diastolic left ventricular area Z-score(LV area Z-score) were compared between the two groups. RFOF fetuses were followed up to observe the prognosis.Results:There were significant differences in fetal ASEI, foramen ovale diameter, foramen ovale valve excursion distance, the ratio of foramen ovale diameter to total atrial septum length, AOV Z-score, PV Z-score, AOA Z-score, AOI Z-score, AOD Z-score and LV area Z-score between RFOF group and control group (all P<0.05), but there was no significant difference in RV area Z-score ( P>0.05). Postnatal follow-up outcomes in RFOF group indicated that 17 of 22 cases (77.3%) had live birth, five cases (22.7%) were lost at follow-up. Twelve of 17 live birth cases (70.5%) showed structurally normal heart by postnatal echocardiography, 3 cases (17.6%) showed mild coarctation of the aorta with nonsignificant hemodynamics under clinical follow-up, 2 cases (11.8%) were diagnosed with coarctation of the aorta, pulmonary artery dilation and atrial septal defect, and died after operation, of which one case died of infections pneumonia after operation. Another case was born prematurely at 36 + 4 weeks with a birth weight of 2 650 g, and neotal echocardiography showed ventricular septal defect, atrial septal defect and patent ductus arteriosus. Conclusions:ASEI can quantitatively evaluate the behavior of foramen ovale valve mobility and in combination with Z-scores of cardiovascular structures can be used to assess the morphological changes of fetal hearts. Although some fetuses with RFOF demonstrate left and right ventricular disproportion and coarctation of the aorta in fetal echocardiography, most fetuses have a good prognosis.

Linking microbial diversity with ecosystem functioning through a trait framework

Trait-based microbial biogeography provides a path for the mechanistic understanding of relationships between microbialdiversity and ecosystem functioning. In this mini-review, we identify the scenarios in which to use traits to characterize themicrobial community and propose a simple trait-based conceptual framework to link microbial communities withecosystem functions.

Evaluation of Inferior Capsular Laxity in Patients with Atraumatic Multidirectional Shoulder Instability with Magnetic Resonance Arthrography

OBJECTIVE: To compare inferior capsular redundancy by using magnetic resonance arthrography (MRA) images in patients with multidirectional instability (MDI) of the shoulder and control subjects without instability and thereby develop a screening method to identify the presence of shoulder MDI. MATERIALS AND METHODS: The MRA images of patients with MDI of the shoulder (n = 65, 57 men, 8 women; mean age, 24.5 years; age range, 18–42 years) treated over an eight-year period were retrospectively reviewed; a control group (n = 65, 57 men, 8 women; mean age, 27.4 years; age range, 18–45 years) without instability was also selected. The inferior capsular redundancy was measured using a new method we named the glenocapsular (GC) ratio method. MRA images of both groups were randomly mixed together, and two orthopedic surgeon reviewers measured the cross-sectional areas (CSAs) and sagittal capsule-head ratios on oblique sagittal images, as well as the axial capsule-head ratios on axial images and GC ratios on oblique coronal images. RESULTS: The CSAs and GC ratios were significantly higher in patients than in controls (both, p 1.42 was found to be most suggestive of MDI of the shoulder, owing to its high sensitivity (92.3%) and specificity (89.2%). CONCLUSION: GC ratio can be easily measured and used to accurately screen for MDI of the shoulder.

Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6

Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O. Conclusion: The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.

Analysis of global ecology of Acanthopanax senticosus in suitability and quality / 药学学报

Geographic information system for global medicinal plants (GMPGIS) and MaxEnt modeling are adopted to analyze the ecological suitability of the endangered plant Acanthopanax senticosus. Response curves were created by the raster data of 6 ecological factors, including mean annual temperature, mean temperature of warmest quarter, mean temperature of coldest quarter, mean annual precipitation, mean annual humidity, and mean annual radiation. The relationship between the syringin content of this plant and these ecological factors was analyzed using a redundancy analysis method (RDA), which could be used to predict the most relevant ecological factors influencing the active constituents of Acanthopanax senticosus plants. GMPGIS and MaxEnt results suggest that China, Russia, Japan, and North Korea, are the main producing areas in the world for Acanthopanax senticosus, while there are also other potential areas with maximum similarities of ecological distribution in the United States, Canada, Ukraine, Romania, Hungary, Germany and 22 other countries. In addition, the genuine producing areas in China mainly include Heilongjiang, Jilin and Liaoning, while there are the maximum similarities of ecological distribution of Acanthopanax senticosus in Hebei, Shanxi, Shaanxi and Sichuan. RDA results suggest that the mean annual humidity, precipitation, temperature are the most important eco-factors positively affecting the content of syringin in Acanthopanax senticosus. Our research provides scientific support to the utilization of ecological suitability areas for endangered plant Acanthopanax senticosus and the resource regeneration.

Evaluación psicométrica de la psicopatía: una revisión metaanalítica / Psychometric assessment of psychopathy: A meta-analytical review

Resumen La mayoría de la literatura sobre la psicopatía gira en torno a su medida. Cuatro son los instrumentos y familias de referencia en la evaluación de la psicopatía: la Psychopathy Checklist (PCL), la Levenson's Primary and Secondary Psychopathy Scales (LPSP), la Self-Report Psychopathy Scale (SRP), y el Psychopathic Personality Inventory (PPI). Ante este estado de la cuestión, nos planteamos llevar a cabo un metaanálisis psicométrico correlacional con el cual someter a prueba la validez convergente de estos instrumentos a nivel unidimensional y en los dos factores comunes a todos ellos: el factor afectivo y el factor antisocial. Los resultados mostraron una validez convergente positiva, significativa y generalizable entre los instrumentos, tanto en la dimensión psicopatía como en los factores afectivo y antisocial. Asimismo, los resultados pusieron de manifiesto que la PCL es el instrumento más idóneo para la evaluación de la psicopatía en poblaciones clínicas y el PPI en población subclínica.

Abstract Most of the literature on psychopathy is focused on its measurement. There are four reference tools used to assess psychopathy: the Psychopathy Checklist (PCL), the Levenson's Primary and Secondary Psychopathy Scales (LPSP), the Self-Report Psychopathy Scale (SRP), and the Psychopathic Personality Inventory (PPI). In order to do this, a psychometric correlational meta-analysis was designed to test the convergent validity between these tools on the psychopathy dimension and the two common factors among them: the affective and antisocial deviation factors. The results showed a positive, significant and generalisable convergent validity between tools, in the psychopathy dimension, as well as in the affective and antisocial factors. Moreover, the results also showed that the PCL was the most appropriate tool to measure psychopathy in clinical samples, and the PPI was so in sub-clinical cases.

Planos de demissão voluntária: reflexos sobre a relação indivíduo-trabalho / Voluntary redundancy plans: repercussions over the relation individual-work

O presente trabalho marca uma das etapas de produção da dissertação do mestrado desenvolvida junto ao Programa de Pós-Graduação em Psicologia da UFC. Diante do momento de marcada instabilidade econômica no cenário brasileiro, representada por fatores como o aumento da inflação, o recuo no crescimento de numerosos setores da economia e a grande redução de postos de trabalho, vimos ressurgir estratégias organizacionais que visam a manutenção dos lucros, alicerçadas em ações que permitam mudanças rápidas e significativas para os empreendimentos que as adotam. Tem-se como objetivo investigar a utilização de uma das estratégias que reapareceu nesse momento de crise, a saber, os Planos de Demissão Voluntária (PDVs). Historicamente, a intensificação de sua aplicação no Brasil se deu no decurso da década de 1990, caracterizada pela adoção de medidas político-econômicas neoliberais, ademais da política de privatização de empresas nacionais. Através de uma revisão bibliográfica, analisaremos os desdobramentos da tentativa de implantação do projeto neoliberal no Brasil, tomando como foco as transformações na dinâmica do trabalho no contexto nacional e suas principais consequências para os trabalhadores. Buscaremos refletir acerca da utilização dos PDVs enquanto mecanismos de desmantelamento do setor público à época, e sobre seus objetivos e possíveis repercussões na atualidade.

The present paper marks one of the stages of production of a master's dissertation developed along the Psychology's Post-Graduation Program of the Federal University of Ceará - UFC. Facing the moment of marked economic instability in the brazilian scenario, represented by factors such as the increase of inflation, the decline of economic growth in many sectors of the economy and the great reduction of job offers, we saw the resurgence of organizational strategies that aim to maintain profits, grounded in actions that allow quick and significant changes for the enterprises that adopt them. Our objective is to investigate the use of one of those strategies that reapeared in this moment of crisis, namely, the Voluntary Redundancy Plans. Historicaly, the intensification of its aplication in Brazil ocurred during the 1990's, characterized by the adoption of neoliberal political and economic measures, in addition to the privatization of national companies. Through a bibliographic review, we'll analyze the unfold of events that followed the attempt of implementation of the neoliberal project in Brazil, taking as focus the transformations in the work dynamics in the national context and the main consequences for workers. We seek to reflect about the use of voluntary redundancy while a dismantling mechanism of the public sector at the time and about its objectives and possible repercussions in the present.

The Impact of Redundancy and Teamwork on Resilience Engineering Factors by Fuzzy Mathematical Programming and Analysis of Variance in a Large Petrochemical Plant

BACKGROUND: Resilience engineering (RE) is a new paradigm that can control incidents and reduce their consequences. Integrated RE includes four new factors—self-organization, teamwork, redundancy, and fault-tolerance—in addition to conventional RE factors. This study aimed to evaluate the impacts of these four factors on RE and determine the most efficient factor in an uncertain environment. METHODS: The required data were collected through a questionnaire in a petrochemical plant in June 2013. The questionnaire was completed by 115 respondents including 37 managers and 78 operators. Fuzzy data envelopment analysis was used in different α-cuts in order to calculate the impact of each factor. Analysis of variance was employed to compare the efficiency score means of the four above-mentioned factors. RESULTS: The results showed that as α approached 0 and the system became fuzzier (α = 0.3 and α = 0.1), teamwork played a significant role and had the highest impact on the resilient system. In contrast, as α approached 1 and the fuzzy system went toward a certain mode (α = 0.9 and α = 1), redundancy had a vital role in the selected resilient system. Therefore, redundancy and teamwork were the most efficient factors. CONCLUSION: The approach developed in this study could be used for identifying the most important factors in such environments. The results of this study may help managers to have better understanding of weak and strong points in such industries.

Fish functional groups in a tropical wetland of the Yucatan Peninsula, Mexico

La caracterización de los rasgos funcionales de las especies es un paso importante en la comprensión y descripción de las comunidades en hábitats naturales. La clasificación de especies en grupos funcionales es una herramienta útil para identificar la redundancia y la especialización. Se estudió la comunidad de peces de un humedal prístino de agua dulce en la Reserva de la Biosfera de Sian Ka'an, analizando dos funciones multidimensionales: adquisición de alimento y la locomoción. Analizamos los cambios en la estructura de los grupos funcional entre hábitats (permanentes y temporales) y temporadas (lluvias y secas). Seis grupos funcionales con características ecológicas distintas fueron detectados, dos de los cuales presentaron una alta redundancia funcional y tres de ellos están representados únicamente por una especie con una función ecológica particular. En las pozas permanentes durante la temporada de secas, la riqueza y diversidad de grupos funcionales fueron menores; mientras que la equitatividad de grupos funcionales fue mayor. Durante la temporada de lluvias se detectaron todos los grupos funcionales y la estructura de los grupos fue similar entre los hábitat. Estos resultados sugieren que los filtros ambientales tienen un efecto durante la temporada de secas y la complementariedad de nicho durante la temporada de lluvias.

The characterization of species' functional traits is a major step in the understanding and description of communities in natural habitats. The classification of species into functional groups is a useful tool to identify redundancy and uniqueness. We studied the fish community of a pristine freshwater wetland in the Sian Ka'an Biosphere Reserve by analysing two multidimensional functions: food acquisition and locomotion. We investigated changes in the functional group structure between habitats (permanent and temporary pools) and seasons (dry and wet). Six functional groups with different ecological characteristics were detected, two of which had high functional redundancy and three of them were represented by single species with unique ecological functions. In permanent pools during the dry season, functional group richness and diversity were lower, while evenness was higher. During the wet season, all functional groups were detected and similar functional group structure was found between habitats. These results suggest an effect of environmental filtering during the dry season and niche complementarity during the wet season.

An analysis of vascular system in the compound tendrilled afila leaf in Pisum sativum.

Recent work on the venation patterning and morphogenesis of leaf/leaflet has posed the question how different are these in tendrils, which are another type of vegetative lateral organ. Here, the venation patterns of leaflets, stipules and tendrils were compared in the model species, P. sativum. Unlike reticulated venation in leaflets and stipules, venation in tendrils comprised of one or more primary veins. A few secondaries were attached to a primary vein, mostly distally. Bilaterally symmetrical secondary veins were rare. The primary veins in tendrils were daughter strands from dichotomously divided mother veins in rachis, connected finally to vascular strands in stem. A tendril received primary vein from one or more mother strands. Some mother strands contributed primary veins to proximal, distal and terminal domain tendrils of af leaf. The tendrils shared the multi-primary vein character with stipules. Vein redundancy provided a mechanism for survival of tendril/leaf against injury to some of the veins/mother veins. The presence of aborted primary veins that did not reach apex, rows of cambium cells attached to primary vein(s) at apex, the pattern of attachment of primary veins to mother veins and cessation of vein growth in apical direction in aborted tendrils of af lld genotype indicated that the growth of primary veins and tendril was acropetal. Loss-of-function of AF extended the repression of TL and MFP genes on leaflet development from distal and apical domains to proximal domain of leaves in af mutants.

Triple redundant signals effect in the visual modality / Efecto de señales triple redundantes en la modalidad visual

Response times in a visual object recognition task decrease significantly if targets can be distinguished by two redundant attributes. Redundancy gain for two attributes has been commonly found, but redundancy gain from three attributes has been found only for stimuli from three different modalities (tactile, auditory, and visual). This study extends those results by showing that redundancy gain from three attributes within the same visual modality (color, form and direction of movement) is possible. It also presents evidence that the separate activation model cannot account for such a gain.

Los tiempos de respuesta en las tareas de reconocimiento visual de objetos disminuye significativamente si los objetivos pueden ser distinguidos por dos atributos redundantes. La ganancia de redundancia para dos atributos se ha encontrado comúnmente, pero la ganancia de redundancia de tres atributos ha sido encontrada solo para estímulos desde tres modalidades diferentes (táctil, auditivo y visual). Este estudio se extiende a aquellos resultados mostrando que el aumento de la redundancia es posible en tres atributos dentro de la misma modalidad visual (color, forma y dirección del movimiento). También se presenta evidencia de que el modelo de activación separada no puede dar cuenta de una ganancia como tal.

SAD phasing with in-house Cu Kα radiation using barium as anomalous scatterer.

Phasing of lysozyme crystals using co-crystallized barium ions was performed using single-wavelength anomalous diffraction (SAD) method using Cu Kα radiation with in-house source of data collection. As the ion binding sites vary with respect to the pH of the buffer during crystallization, the highly isomorphic forms of lysozyme crystals grown at acidic and alkaline pH were used for the study. Intrinsic sulphur anomalous signal was also utilized with anomalous signal from lower occupancy ions for phasing. The study showed that to solve the structure by SAD technique, 2.8-fold data redundancy was sufficient when barium was used as an anomalous marker in the in-house copper X-ray radiation source for data collection. Therefore, co-crystallization of proteins with barium containing salt can be a powerful tool for structure determination using lab source.

Programming and rebuild of network system in hospital information construction / 医疗卫生装备

Objective To construct a safe,stable and credible system for the running of network system and improve its application effect.Method Starting with the information system construction in Xijing hospital,the existent network system was programmed and rebuilt by establishing a assistant switch core and constructing multi-center redundancy link.Conclusion The rebuilt information system of Xijing hospital attains the anticipated effect,and shows rationality and practicability in use.What's more,combined with actual experience,the rebuild of hospital network system was summarized and suggestions were given.

Multivariate Analysis Of Dermatoglyphic Whorl Patterns.

Triangles were constructed on dermatoglyphic whorl patterns by joining the cores and triradii. Several angles were measured, and the correlation among these measurements and the ridge counts war studied. Descriptive statistics and canonical correlations were also computed. The major findings, included a significant positive correlation between the radial / ulnar tangent angle and the radial / ulnar ridge count and a significant negative correlation between the radial / ulnar base angle and the radial / ulnar ridge count.