ABSTRACT
Objective:HPLC was used for the determination of galangin and kaempferol of Rhizoma Alpiniae Officinarum.Methods:The column was phenomenex Gemini C 18 (5?,250mm?4.6mm);the mobile phases were methanol-0.4% phosphoric acid(60:40);the detection wavelength was 360 nm;the flow rate was at 1.0 ml/min and the column temperature was at 30?C respectively.Results:Galangin showed a good linear relationship in the range of 0.668-6.680?g,r=0.9996,and the average recovery was 102.30%with RSD=1.73%(n=6);Kaempferol also showed a good linear relationship in the range of 15.5-155.0ng,r=0.9995,and the average recovery was 97.31%with RSD 1.84%(n=6).Conclusion:This method was simple,accurate,reliable and sensitive and it can be used as a quality control standards for Rhizoma Alpiniae Officinarum.
ABSTRACT
Objective: To study the optimum extraction condition of effective composition in Rhizoma Alpiniae Officinarum by CO 2 supercritical fluid extraction. Methods: CO 2 supercritical fluid extraction condition was selected by uniform design. The extraction pressure, extraction temperature, analytic pressure, analytic temperature were four factors in the experiment. Seven levels of each factor were selected.Results: The optimum extraction condition in SFE was: extraction pressure at 30MPa, extraction temperature at 32?C , analytic pressure at 6MPa, analytic temperature at 36?C . Conclusion: The method is simple, selective and efficient.