ABSTRACT
Objective To study the influence of Rhizoma typhonii extract on the growth of glioma SHG-44 cells cultured invitro,and to explore the mechanism of the inhibitory effect of Rhizoma typhonii extract on the growth of glioma cells.Methods The SHG-44 cells were cultured and divided into blank control group and 8, 40, 200, 1 000μg·L-1 Rhizoma typhonii extract groups.The inhibitory effect of Rhizoma typhonii extract on the growth of glioma SHG-44 cells was measured by MTT assay. The secretion levels of Bax and caspase-3 proteins were examined by ELISA assay. The expression level of caspase-3 protein was examined by Western blotting method. Results Compared with blank control group, the inhibitory rates of the growth of SHG-44 cells in 200, 1 000μg·L-1 Rhizoma typhonii extract groups at 24 h,and 8,40,200,and 1 000μg·L-1 Rhizoma typhonii extract groups at 48 h were significantly increased (P<0.05 or P<0.01).The secretion levels of Bax and caspase-3 proteins in 40,200,and 1 000μg· L-1 Rhizoma typhonii extract groups at 48 h were increased compared with blank control group (P<0.05 or P<0.01 ). Compared with blank control group, the expression levels of caspase-3 protein in different doses of Rhizoma typhonii extract groups were increased significantly (P<0.01). Conclusion Rhizoma Typhonii extract can inhibit the growth of cells through up-regulating the expression of Bax protein,increasing the expression level of caspase-3 protein and activating apoptosis pathway.
ABSTRACT
Objective To study the degradation of Rhizoma Typhonii polysaccharide with sulfuric acid under the normal and ultrasonic condition. Methods The orthogonal test table L9(34) was used to optimize 4 factors including acid concentration, reaction time, reaction temperature and ultrasonic power, with the yield of the low-molecular-weight polysaccharide as the index. Results The optimal conditions of discomposing Rhizoma Typhonii polysaccharide with sulfuric acid were 10% sulfuric acid, 100 ℃ for 2 h under the normal condition,and the yield of low-molecular-weight polysaccharide was 36.17%. Under the ultrasonic condition, the optimal conditions were 150 W of the ultrasonic power, 5% sulfuric acid, 40 ℃ for 1 h, and the yield was 44.63%. Conclusion The degrading yield of the low-molecular-weight polysaccharide can be increased under ultrasonic condition with sulfuric acid.
ABSTRACT
Objective:To compare antitumor activity in vivo of Rhizoma Typhonii before and after processing. Methods:To observe the effect of water decoction and ethanol extract from Rhizoma Typhonii before and after processing on the sarcoma grafts and immune organs of S180 mice by using mice transplant tumor model. Results:The results showed that both of the Rhizoma Typhonii and processed Rhizoma Typhonii had antitumor effect,and the former was stronger than the latter. The extract from processed Rhizoma Typhonii can inhance the immune function of mouse at some extent. Compared with control group,the Rhizoma Typhonii before processing had no effect in prolonging the life of tumor-bearing mouse. But,the ethanol extract from processed Rhizoma Typhonii can prolong the life of S180 mouse. Conclusion:Decoction and ethanol extract from Rhizoma Typhonii and processed Rhizoma Typhonii had antitumor effect. Processed Rhizoma Typhonii can enhance immune function and increase in life span on S180 mice,So processed Rhizoma Typhonii should be used in clinical medicine.