Aprovechamiento alternativo de calamar (Dosidicus gigas) como fuente de proteína para la fortificación de pan tipo baguette / Alternative use of squid (Dosidicus gigas) flour as a protein source to improve the nutritional quality of baguette bread

Introducción. Los cereales tienen aminoácidos limitantes y mezclarlos con harinas de origen marino presentan un mejor equilibrio en éstos mejorando la composición nutricional en productos de panificación, principalmente de proteína y aminoácidos. Objetivo. Desarrollar panes tipo "baguette" sustituyendo parcialente harina de trigo (HT) por harina de calamar (HC) y evaluar su calidad física, química y sensorial. Materiales y métodos. Se utilizó la técnica de masa esponja incorporando 7,5, 10 y 12,5 % de HC. Análisis físicos: tiempo de amasado, peso y fermentación de la masa, volumen específico, peso de los panes (g), % pérdida humedad y color de miga; análisis químicos: humedad, proteína cruda, cenizas, extracto etéreo, fibra cruda, carbohidratos, aminoácidos indispensables y energía bruta y evaluación sensorial: textura, olor, color y sabor. Resultados. Las propiedades físicas no presentaron diferencias (p>0,05), pero sí (p<0,05) para las variables de color en todos los panes, al igual que en los análisis químicos y perfil de aminoácidos (p<0,05), incrementándose la proteína (15,63 % pan con 7,5 % de HC hasta 23,27 % pan con 12,5 % de HC) y mismo comportamiento de aminoácidos indispensables conforme aumentó la inclusión de HC. Los resultados de la evaluación sensorial fueron similares (p>0,05) para 7,5 % de HC (me gusta), para 10,0 y 12,5 % fue indiferente. Conclusiones. La inclusión de HC (7,5 %) en baguette logró un aumento de proteínas y aminoácidos esenciales mejorando la calidad nutricional, con buena aceptación de acuerdo con la prueba de carácter exploratorio con consumidores que se empleó(AU)

Introduction. Cereals have limiting amino acids and mixing them with flours of marine origin could provide a better balance in these improving the nutritional composition in bakery products, mainly protein and amino acids. Objective. To develop and evaluate the physical, chemical, and sensory quality of three white breads of baguette type by partially substitution of wheat flour (HT) with squid flour (HC). Materials and methods. The sponge dough technique was used incorporating 7,5, 10,0 and 12,5 % of HC. Physical analysis: kneading time, dough weight, dough fermentation, specific volume, bread weight (g), % loss of moisture and crumb color; chemical analysis: moisture, crude protein, ash, ether extract, crude fiber, carbohydrates, indispensable amino acids and sensory evaluation: texture, odor, color, and flavor. Results. Physical properties did not show differences (p>0.05), differences (p<0.05) for color variables in all breads as well as in chemical analysis and amino acid profile (p<0.05), the protein show an increase (15,63 % for bread with HT to 23,27 % for bread with 12.5 % of HC) and the same behavior for indispensable amino acids, that increase as inclusion of HC increases. The results of the sensory evaluation were similar (p>0.05) in control and 7,5 % of HC (I like it), for 10 and 12,5% it was indifferent. Conclusions. The inclusion of squid flour (7,5 %) in the preparation of baguette bread achieved an increase of proteins and essential amino acids that improved the nutritional quality of this food, which also presented a good acceptance according to an exploratory test with consumers(AU)

A Case of Effective Zone 0 TEVAR Using Squid-Capture Assisted in situ Stent-Graft Fenestration, for Endoleak from the Fenestration of Najuta / 日本心臓血管外科学会雑誌

We report the case of a 76-year-old man who developed type IA endoleak through the fenestration after 1-debranch TEVAR using a Najuta endograft. The patient was admitted with expansion of the aneurysm after TEVAR, for additional therapy. Type IA endoleak through a fenestration has remained a significant clinical concern and its treatment is challenging. We performed Zone 0 TEVAR using the “Squid-Capture” technique assisted in situ stent-graft fenestration. Cerebral vessels were perfused by a percutaneous cardiopulmonary support system during in situ stent-graft fenestration, and the cerebral branch was clamped at the proximal site. It is difficult to operate the catheter inside the endoskeleton structure of a Najuta endograft, but several innovations were effective. Test dilation of the balloon catheter was performed to ensure that the wire did not interfere with the endoskeleton. Avoiding interference with the endoskeleton is important. The Squid-Capture technique allows safe and secure puncture of the graft. The operation was completed successfully. After this procedure, the endoleak disappeared. It is considered to be a useful method for treatment of endoleak through the fenestration.

Thysanoteuthis rhombus Troschel, 1857 (Teuthida: Thysanoteuthidae) calamar diamante, registro en la zona costera de Ica, Perú / Thysanoteuthis rhombus Troschel, 1857 (Teuthida: Thysanoteuthidae) Diamond squid, registered from coastal area of Ica, Peru

Resumen En el presente trabajo a través de la pesca artesanal se registra por primera vez Thysanoteuthis rhombus en el litoral de Pisco y se amplía su rango de distribución en el pacífico oriental hasta Ica, Perú. Un ejemplar de 68 cm LM fue capturado entre los 13°50'00" S y 77°08'00" W a 50 metros de profundidad. Se realiza una breve descripción de la especie, con observaciones taxonómicas y comentarios sobre su distribución geográfica y pesquería.

Abstract In this paper, Thysanoteuthis rhombus is first registered in the coast of Pisco and its distribution range in the Eastern Pacific is extended to Ica, Peru. A 68 cm LM specimen was captured between 13°50'00"S and 77°08'00"W at 50 meters depth. A brief description of the species is given, with taxonomic observations and comments on its geographic distribution and fishery.

Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

Discussion on Technical Evaluation of Magnetocardiography / 中国医疗器械杂志

There is no corresponding product standard and industry standard for the search and development of magnetocardiograph at home and abroad. In this paper, based on the working principle and clinical application function of magnetocardiograph, and on the basis of relevant regulations and normative documents of medical device registration, some thoughts and concerns about the technical review of this kind of product are put forward.

Efeitos da irradiação gama sobre a estabilidade físico-química de anéis de lula congelados / Effects of gamma irradiation on the physico-chemical stability of frozen squid rings

O principal objetivo deste trabalho foi avaliar os efeitos do processo de exposição à radiação gama (césio- 137) na estabilidade físico-química de anéis de lula, Dorytheutis plei, congelados. Foram investigadas 30 amostras, separadas em três grupos, de acordo com a dose de radiação absorvida: 0 kGy (controle), 1,5 kGy e 3,0 kGy. Análises físico-químicas, de Base Voláteis Totais e pH, relevantes na determinação da qualidade do pescado, foram realizadas durante o período de seis semanas. Durante a estocagem, observou-se uma relativa estabilidade físico-química nas amostras estudadas, caracterizando um produto viável para a comercialização. Dentre as doses investigadas, 1,5 kGy mostrou-se a mais apropriada para tratamento do produto. Não houve alteração significativa da composição centesimal das amostras.

Selective cytotoxicity of bioluminescent bacteria isolated from fresh ink of Philippine squid on human colon cancer and normal cell lines

Aims: The current work determined the cytotoxicity of the symbiont bioluminescent bacteria isolated from fresh ink of the squid, Photololigo duvaucelii for human colon cancer cell lines HT-29 and HCT116 and normal human dermal fibroblasts. Methodology and results: The crude sterile culture supernatants of the bacterial isolates grown in marine broth containing 2.8% of NaCl were tested for their cytotoxic activities for HT-29 and HCT-116 human colon cancer cells and the normal human dermal fibroblasts HDFn using Presto Blue™ Resazurin Assay. Zeocin served as the positive control. The cytotoxicity index profiles of all culture supernatants and negative control (marine broth with 2.8% NaCl) for HDFn suggest non-toxicity to the cells, whereas most culture supernatants were observed to be cytotoxic to the two colon cancer cell lines. The culture supernatants of the isolates were found to be more cytotoxic for the HT-29 colon cancer cells than to the HCT-116 colon cancer cells. At the same time, the IC50 values showed that 85% (17/20) and 40% (8/20) of the culture supernatants tested were significantly lower, hence more potent than zeocin for HT-29 and HCT-116, respectively (p 0.05). The 16S rRNA gene sequence analysis of the bioluminescent isolates studied revealed that they have a 97-99% similarity identity with Photobacterium leiognathi. Conclusion, significance and impact of study: This may be the first report on the cytotoxic activities on cancer cells of P. leiognathi from the Philippine squid and suggests the potential use of the secondary metabolites of these bioluminescent bacteria as anti-cancer agents.

Anisakiasis Involving the Oral Mucosa

Anisakis is a parasite with life cycles involving fish and marine mammals. Human infection, anisakiasis, occurs with the ingestion of raw infected seafood and usually presents with acute or chronic gastrointestinal symptoms from esophageal or gastric invasion. We report a rare caseinvolving the oral cavity. A 39-year-old male presented with oral and sub-sternal pain of one day duration after eating raw cuttlefish. Physical examination revealed areas of erythema and edema with a central white foreign particle on the labial and buccal mucosa. With microscopic field we could remove the foreign material from the lesions. The foreign material was confirmed to be Anisakis. Anisakis was also removed from the esophagus by esophagogastroduodenoscopy. The patient was discharged the following day without complication. Anisakiasis is frequently reported in Korea and Japan, countries where raw seafood ingestion is popular. The symptoms of acute anisakiasis include pain, nausea, and vomiting and usually begin 2–12 hours after ingestion. The differential diagnosis includes food poisoning, acute gastritis, and acute pancreatitis. A history of raw seafood ingestion is important to the diagnosis of anisakiasis. Treatment is complete removal of the Anisakis to relieve acute symptoms and prevent chronic granulomatous inflammation.

Composición química de harina de calamar gigante dosidicus gigas / Chemical composition of giant squid dosidicus gigas meal

El potencial de uso de la harina de calamar gigante (Dosidicus gigas) (HCG). como alternativa en el desarrollo de productos con valor agregado es relevante. Sin embargo hace falta conocer los elementos químicos que la conforman. El objetivo de este trabajo fue determinar la composición química de la harina de calamar gigante (Dosidicus gigas) procedente de Guaymas, Sonora, México y su posible alternativa para el desarrollo de alimentos funcionales. Los resultados indicaron un alto contenido de proteína (77,7%), sobresaliendo lisina y ácido glutámico (10,16 y 14,53 g aa/100g proteína respectivamente), aminoácidos azufrados y aminoácidos hidrofóbicos. El contenido de la fracción grasa (6,3%) fue bajo así como el de fibra cruda (2,7%), reportada como quitina, reflejándose en el bajo aporte calórico (4 kcal/g). La relación entre ácidos grasos saturados, monoinsaturados y poliinsaturados fue de 1,66:1:1,08 y de n6:n3 fue de 1:1,35. Se concluye que HCG es un ingrediente con posibilidades de uso en panificación, galletas saladas, sazonadores, aderezos, a los que les podría dar un valor agregado. Sin embargo el factor limitante para su uso está en el olor y sabor a pescado, por lo que su aplicación se sugiere dirigir la aplicación hacia el desarrollo de nuevos productos vinculados con preparaciones típicas que incluyan pescados y derivados(AU)

The potential use of giant squid (Dosidicus gigas) meal (GSM) as an alternative in the development of value-added foods may be relevant. However one must know the chemical elements that constitute it. The aim of this study was to determine the chemical composition of giant squid Dosidicus gigas meal from Guaymas, Sonora, Mexico and its possible alternative for the development of functional foods. The data indicated a high protein content (77,7%), lysine and glutamic acid (10,16 and 14,53 g aa/100g protein respectively), sulfur amino acids and hydrophobic amino acids. The content of fat fraction (6,3%) was low and crude fiber (2,7%) reported as chitin, reflected in the low calorie (4 kcal/g). The ratio of saturated, monounsaturated and polyunsaturated fatty acids was 1,66: 1: 1,08 and n6: n3 was 1: 1,35. It is concluded that GSM is an ingredient with potential for use in bread, crackers, seasonings, dressings, which could give them added value. However the limiting factor for use is in the smell and taste of fish, so that its application would be directed at the development of new products related with typical preparations that include fish and derivatives(AU)

Plerocercoids of Nybelinia surmenicola (Cestoda: Tentacularidae) in Squids, Todarodes pacificus, from East Sea, the Republic of Korea

A visceral helminth of the squid, Todarodes pacificus, is reported from the East Sea, the Republic of Korea. Total 39 squid samples were purchased from a fish market in Jumunjin-eup, Gangneung-si (City) from August 2014 to July 2015 and were examined for helminth parasites with naked eyes and under a stereomicroscope after opening the abdominal cavity with a pair of scissors. Whitish larval worms were mainly found in the stomach and abdominal cavity of the squid. They were detected in 25 (64.1%) out of 39 squids examined, and the infection density was 7 larvae per infected squid. Spatula-shaped larvae were 8.2×2.0 mm in average size, round to slightly flattened anteriorly, with round hatching posteriorly, and had characteristic 4 tentacles with numerous hooklets in the scolex. The larvae were identified as the plerocercoid stage of Nybelinia surmenicola by their morphological features. This finding represents a new host record and the first report of N. surmenicola infection in T. pacificus squids from the east coast of Korea.

The Kraken: when myth encounters science / O Kraken: quando mito e ciência se encontram

Hundreds of years ago, sailors were terrified by the Kraken, a dreadful sea monster capable of sinking ships and with a taste for human flesh. Today we know the legends of this monster were based on sightings of giant squids. This animal belongs to the genus Architeuthis and was the subject of many scientific studies. Despite its enormous size (up to 18m), the giant squid is astoundingly elusive and much of its biology remains unknown. Thus shrouded in mystery, Architeuthis is almost a mythological creature and has a place both in science and in myth: the very last of the legends to persist to this day.

Séculos atrás, marinheiros se amedrontavam com histórias do Kraken, um terrível monstro marinho capaz de afundar embarcações e devorar suas tripulações. Atualmente, sabemos que a lenda desse monstro foi baseada em encontros com lulas-gigantes. Esse animal pertence ao gênero Architeuthis e foi alvo de muitos estudos científicos. Apesar de seu enorme tamanho (pode chegar a 18m), a lula-gigante é incrivelmente elusiva e muito de sua biologia permanece desconhecida. Assim, envolto em mistério, Architeuthis é quase um ser mitológico, ocupando um lugar tanto na ciência como no mito: a última das lendas a persistir nos dias de hoje.

Are deep-sea cephalopods really common preys for oceanic seabirds? / Cefalópodes de profundidade são realmente presas comuns para aves marinhas oceânicas?

An analysis of published data on oceanic seabirds diets, show the predominance of muscular cephalopods with superficial distribution in the oceanic layers, but also important are the gelatinous and ammoniacal species restrict to layers below 300 m from the surface. In principle, it could be not expected that deep-sea cephalopods are common prey for seabirds like several authors have been concluded. It is proposed in this study that an indirect source, important and easily attainable, have been appeared with the beginning of tuna longline operations. The habit to feed upon viscera of the fishes captured by tuna longliners, that discard the gut contents to the water, may explain the probable equivocal conclusions that deep dwelling cephalopods are natural prey of oceanic seabirds.

Uma análise de dados publicados sobre dietas de aves marinhas oceânicas mostra a predominância de cefalópodes musculares e de distribuição mais superficial nas camadas oceânicas, mas também são importantes as espécies gelatinosas e amoniacais restritas a camadas abaixo dos 300 m da superfície. A princípio, não deveria se esperar que cefalópodes de profundidade fossem considerados presas comuns de aves marinhas oceânicas como reportados por muitos autores. É proposto neste estudo que uma fonte indireta, importante e de fácil obtenção, surgiu com o início das atividades dos barcos atuneiros que operam com espinhel. O hábito de ingerir restos de vísceras de peixes capturados em barcos espinheleiros pode explicar as prováveis conclusões equivocadas de que cefalópodes de profundidade são presas naturais de aves marinhas oceânicas.

Production of valued materials from squid viscera by subcritical water hydrolysis.

Subcritical water hydrolysis was carried out to produce valued materials from squid viscera, the waste product of fish processing industries. The reaction temperatures for hydrolysis of raw and deoiled squid viscera were maintained from180 to 280oC for 5 min. The ratio of material to water for hydrolysis was 1:50. Most of the proteins from deoiled squid viscera were recovered at high temperature. The protein yield in raw squid viscera hydrolyzate decreased with the rise of temperature. The reducing sugar yield was higher at high temperature in subcritical water hydrolysis of both raw and deoiled squid viscera. The highest yield of amino acids in raw and deoiled squid viscera hydrolyzates were 233.25±3.25 and 533.78±4.13 mg g-1 at 180 and 280oC, respectively. Most amino acids attained highest yield at the reaction temperature range of 180-220oC and 260-280oC for raw and deoiled samples, respectively. The recovery of amino acids from deoiled squid viscera was about 1.5 times higher than that of raw squid viscera.

Effects of collagen polypeptides from squid(Dosidicus gigas)skin on melanogenesis in B16 melanoma cells / 中国药理学通报

10000 u,SP2:6 000 u0.05),the melanogenesis and tyrosinase activity were inhibited remarkably(P

Establishment and Magnetocardiographic Changes of Pressure Overload Left Ventricular Hypertrophy and Its Regression in Rats

BACKGROUND AND OBJECTIVES: Regression of left ventricular hypertrophy (LVH) is important because development of myocardial ischemia, heart failure or arrhythmias may be reduced. However, an animal model for LVH regression is not well established and there are no useful parameters to predict LVH regression. Magnetocardiogram (MCG), magnetic signal generated from the heart, has recently been investigated for the detection of electrical current changes of the heart. This study was undertaken to establish rat models of LVH-regression and to assess MCG changes during LVH induction and regression. MATERIALS AND METHODS: Rat models of pressure overload LVH were established by transverse aortic constriction (TAC) and LVH regression was generated by untying 2 weeks after TAC. Hemodynamic, echocardiographic and biochemical evaluation were performed in order to confirm this model. Magnetic fields were recorded with a SQUID gradiometer before and after TAC, and also recorded at 1, 3, 7, and 14 days after untying, respectively. RESULTS: Rat models of LVH-regression were established successfully by TAC and untying. The pressure gradient across TAC disappeared within 10 minutes after untying. LV weight, LV weight/body weight ratio, LV mass and expression level of atrial natriuretic factor were significantly increased following TAC and decreased to baseline value after pressure unloading. Deeper S waves and strain patterns were observed after LVH induction and gradually returned to basal levels over the 2 weeks after untying. CONCLUSION: MCG changes in the rat models of LVH-regression indicate that MCG can be a helpful modality for the diagnosis and evaluation of LVH as well as follow-up after treatment of LVH.

Sequential changes of magnetocardiogram in rat models of experimental myocardial infarction / 대한내과학회지

BACKGROUND: Magnetocardiogram (MCG), which records the changes of magnetic fields generated by the heart's electrical activity, theoritically can provide unique data for clinical application. To date, MCG has been investigated only at a single time point after myocardial infarction (MI) with severe left ventricular dysfunction in rats. The purpose of the present study was to investigate sequential changes of MCG after MI and to evaluate effects of infarct size on MCG. METHODS: Acute MI were induced by the permanent ligation of left coronary artery in 22 rats. Magnetic fields were recorded just above a rat with Nb Superconducting Quantum Interference Device (SQUID) gradiometer inside a magnetically shielded room. MCG was measured before and immediately after surgery and it was subsequently recorded at the time points of 1, 4 and 6 hours postoperatively. MCG was also measured at 1, 3, 7 and 21 days after surgery. RESULTS: Elevation of ST segment and appearance of pathological Q wave on the MCG were evident immediately after the ligation of coronary artery and persisted to 6 hours after MI. On MCG, ST segment was depressed and T wave was inverted from 1 day after MI. In rats with small- and moderate-sized MI (infarct size or = 30%). CONCLUSION: Evolutional changes of MCG were well-recognized up to 21 days after MI. Furthermore, the infarct size can be expressed by the extent of Q wave and ST segment depression on MCG. Taken together, these data indicate that MCG is a helpful modality for the diagnosis, evaluation of infarct size and follow up after MI.

Studies on the antioxidation activity of squid(Dosidicus eschrichitii Steenstrup) skin gelatin hydrolysate / 中国海洋药物

Objective To investigate the free radical scavenging effects and antioxidation activity of squid(Dosidicus eschrichitii Steenstrup) skin gelatin hydrolysate.Methods The free radical scavenging effects of the hydrolysate on superoxide and hydroxyl free radicals were assessed by chemiluminescence analysis method.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and content of malondialdehyde(MDA),hydroxyprolyne(Hyp) in blood serum and skin tissue in galactose treated mice were analyzed after 42 days of hydrolysate feeding.Results and Conclusion The fraction with relative molecule weight less than 2000 D of the hydrolysate had the highest free radical scavenging effects on superoxide and hydroxyl free radicals,and the activities of SOD,GSH-Px were enhanced,the quantity of MDA in blood serum and skin tissue of the mice was decreased and the quantity of Hyp in skin tissue of mice was increased after the hydrolysate administration.