ABSTRACT
Objective: To optimize the extracting technology for active fractions from Saxifraga stolonifera with anti-prostate cancer activity. Methods: Using the extraction rates of bergenin, quercetin-5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids as indexes, the single factor experiment combined with Box-Behnken design was used to optimize the extracting technology. Results: The optimal extraction conditions were as follows: ethanol volume fraction 60.04%, liquid-solid ratio of 1:13.79 for 89.98 min, and at the temperature of 80°C. Under these conditions, the extraction rates of bergenin, quercetin-5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids were 97.15%, 98.13%, 98.37%, 97.82%, and 96.33%, respectively. Conclusion: The response surface analysis could optimize the extracting technology of active fraction from S. stolonifera with the anti-prostate cancer activity, which provides the basis for the comprehensive utilization and industrialization of S. stolonifera.