ABSTRACT
Objective:To investigate the expressions of liver receptor homolog 1 (LRH-1) and scavenger receptor B type Ⅰ (SRBI) gene in cholesterol gallstone (CGS) mice.Methods:Forty C57BL/6 mice,20 with cholesterol gallstone (GS)and 20 controls without gallstones (GSF) were enrolled in this study.mRNA and protein expression of LRH-1 and SRBI genes were determined by RT-PCR and Western blot.Results:Gallbladder walls of GS mice were thicker with increased stromal granulocyte infiltration.The expression levels of LRH-1 genes were significantly higher in GS mice than in controls(P＜0.01).The expression levels of SRBI genes were also significantly higher in GS mice than in controls(P＜0.01).Conclusion:The increased expression of LRH-1 and SRBI gene may be related to GS disease.
ABSTRACT
Objective:To investigate the expressions of liver receptor homolog 1 (LRH-1) and scavenger receptor B type Ⅰ (SRBI) gene in cholesterol gallstone (CGS) mice.Methods:Forty C57BL/6 mice,20 with cholesterol gallstone (GS)and 20 controls without gallstones (GSF) were enrolled in this study.mRNA and protein expression of LRH-1 and SRBI genes were determined by RT-PCR and Western blot.Results:Gallbladder walls of GS mice were thicker with increased stromal granulocyte infiltration.The expression levels of LRH-1 genes were significantly higher in GS mice than in controls(P＜0.01).The expression levels of SRBI genes were also significantly higher in GS mice than in controls(P＜0.01).Conclusion:The increased expression of LRH-1 and SRBI gene may be related to GS disease.
ABSTRACT
Scavenger receptor B type Ⅰ (SR-BI) is a HDL receptor which fivst identified at molecular level. It can mediate the metabolism of cholesterol and other lipids between HDL and cells, involving in intestinal absorption of cholesterol, and mediate outflow of cholesterol from peripheral tissues to promote the selective uptake of cholesterol by the liver and transfer in liver cells, and then secrete cholesterol into the bile through the bile duct side of the membrane. This process can affect cholesterol levels in bile. In this review,we discuss the process of SR-BI-mediated cholesterol metabolism.