ABSTRACT
Objective Isolation , purification and structure identification of phthalides from A pium graveolens L . Methods Silica gel column and preparative TLC chromatography were used to isolate and purify the phthalide compounds .The structures of those compounds were determined through spectral analysis .Results Three pure compounds were identified as 3-n-butylphthalide ,sedanenolide and sedanolide .Conclusion Three pure phthalide compounds can be used as analytical reference standard for quantitative analysis of A .graveolens .
ABSTRACT
Objective: To develop a quantitative analysis of multi-components with single marker (QAMS) and validate its accuracy and feasibility for simultaneously determining 3-n-butylphthalide (b), sedenenolide (sen), and sedanolide (san) in celery seed extracts (CSE). Methods: Three main effective components,3-n-butylphthalide, sedenenolide, and sedanolide, were selected as analytes for evaluating the quality of CSE. The relative correction factors (RCF, f) of 3-n-butylthalide to the other two thalides were calculated: fb/sen = 0. 226, fb/san = 0.702. The external standard method was used to determine the title compounds in 6 batches of CSE and the results were compared with those of QAMS. Results: The RSD of RCF calculated by different instruments was between 4.4%-6.7%. The RSD of RCF calculated by the same instrument with different chromatographic columns was between 2. 3%-3. 6%. The established RCF had a good reproducibility. The quantitative results of three thalides determined by external standard method and QAMS method were not significantly different. Conclusion: The present method can serve as a new mode to determine multi-components in CSE when standard substances are unavailable.