ABSTRACT
Objective To perform the separation and confirmation of mixed semen stains with immunological test method, and find a more effective method for the detection of mixed semen stains. Methods The semens of three volunteers were mixed. The mixed semen stains were processed and tested with prostate-specific antigen （PSA） colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection, respectively. Statistics of the results of STR were gathered and compared with those of a single semen stain. Results After PSA colloidal gold immunoassay strip method testing, the samples showed a purplish red line in the test area and the control area. The results obtained with the immunomagnetic beads method showed a more complete and effective short tandem repeat （STR） sequence. The mixed semen stains were processed with laser capture microdissection and low volume amplified. The results were summarized and superimposed to obtain a complete single typing, which matched the single semen stain typing, with a typing success rate of 84.00%. Single suspect Y-STR typing was obtained with the application of the method above in actual cases, which provided evidence basis for rapid solving of the case. Conclusion The combination of PSA colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection can be used to separate and confirm the mixed semen stains.
Subject(s)
Humans , Male , Coloring Agents , DNA Fingerprinting , Forensic Medicine , Immunologic Tests , Microsatellite Repeats , SemenABSTRACT
The authors have worked on a technique using pressure sensitive or sticky tape for determination of spermatozoa from a seminal stain. By simply pressing the scotch tape on the stain or gently touching a cotton swab stain to the sticky surface of the tape spermatozoa from the stain can be transferred to the sticky surface. Nest the sticky surface of the tape is covered with a microscopic slide. Third, one end of the tape is firmly fixcal with an adhesive paper band and set up when the slide is vertically out into a coping jar for staining. During the staining process the lower part of the tape may partially free itself from the slide. The modified haematoxylene eosin staining method is recommended. After staining, press the whole tape firmly on the slide in order to get rid of any small air bubbles underneath. Keep the slide dry and in warm place and examine directly under microscope for determination of spermatozoa. This slide does not necessary need any cover glass on it. Comparing yhis new technique with NH4OH soaking of the stain, this technique shows good reliability.
ABSTRACT
The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm.The results of ELISA method of 10 fresh seminal fluid samples and 15 seminal stains showed 100% positive rate. Even diluted the fresh seminal fluid to 10~6 times of seminal stains extract to 51?0~5 times,both were positive also.However,it was negtive for testing other specimens such as saliva stains,urine stains,colostrum stains,sweat stains, vaginal secretion stains and human seminal stains after vesoligation. The result certificate that using specific antibody to the sperm,the ELISA method is high sensitive and specific for the sperm antigen assay.
ABSTRACT
A simple rapid and sensitive Dot-ELISA method was establishhed for identifying the human seminal stains by using anti-human semen monoclonal antibody (A10C6)labeled with horseradish Peroxidase of grayish color revealed a positive reaction. The results showed that the extract of human semen was positive. No Cross-reaction was observed when human tissues body fluids and animal seminal stains were tested.