ABSTRACT
Objective To identify the genetype of the PS1/APP double transgenic mouse model,analyse the tissue histology and detect the formation of the senile plaque.Methods The specific primer was designed and the APP gene was amplified by PCR from the mouse genome DNA.The A? deposits,activation of microglia and astrocyte were observed by the congo red staining and immunohistologic methods.Results As compared to the negative control,the PS1/APP mouse displayed A? deposits restricted to cortical and hippocampal structures,the activated microglia and astrocyte surrounded the plaque,thus the classic senile plaque stucture was formed.Conclusion The double transgenic PS1/APP mouse produced by the two mice we bought from American Jackson Laboratory could simulate the specific pathogenesis of Alzheimer disease and be the efficient experimental animal model.
ABSTRACT
Aim The pharmacodynamics and mechanism of Kadsura heteroclita on the Alzheimers disease (AD) model mice were studied. Methods The Kunming mice were treated with D-galactose and AlCl 3(90 d)to make the AD animal model. The therapy groups were treated with HS 2 (po, 40 d) respectively since d51. The Morris water-maze test, the expressions of PS1, BACE, as well as the pathological observation of the hippocampus and cerebral cortex were carried out to evaluate the effect of HS 2 on the AD model mice. Resluts By oral administration with HS 2, the capacity of learning and memory of the AD model mice was improved. HS 2 can downregulate the expression of PS1, BACE and decrease the amount of senile plaque in their brains. Conclusion HS 2 can meliorate AD model mice's learning and memory deficiency as well as decrease the mRNA content of PS1, BACE and senile plaque in their brains.
ABSTRACT
Alzheimer's disease (AD) is the most common etiology of dementia, that has not been previously reported in Korea. We have experienced two cases of Alzheimer's disease, one occurred in a 53-year-old man with dementia and the other occurred in a 36-year-old woman with a family history of early onset dementia. A neocortical biopsy was done to rule out the cause of dementia and showed diffusely scattered numerous senile plaques and neurofibrillary tangles in cortex of both cases. Interestingly, GFAP, an immunohistochemical stain showed strong positivity in neuritic plaques and the surrounding fibrillary gathering.
Subject(s)
Adult , Female , Humans , Middle Aged , Alzheimer Disease , Biopsy , Dementia , Korea , Neurofibrillary Tangles , Plaque, AmyloidABSTRACT
Alzheimer's disease (AD) is the most common etiology of dementia, that has not been previously reported in Korea. We have experienced two cases of Alzheimer's disease, one occurred in a 53-year-old man with dementia and the other occurred in a 36-year-old woman with a family history of early onset dementia. A neocortical biopsy was done to rule out the cause of dementia and showed diffusely scattered numerous senile plaques and neurofibrillary tangles in cortex of both cases. Interestingly, GFAP, an immunohistochemical stain showed strong positivity in neuritic plaques and the surrounding fibrillary gathering.