ABSTRACT
OBJECTIVE:To develop a method for the determination of midazolam in child plasma.METHODS:Plasma level of midazolam in twenty two ICU children with mechanical ventilation was detected by solid-phase extraction-HPLC after treatment with midazolam for 24h.The separation was performed on Zorbax Eclipse XDB C18 column with acetonitrile — 7.56mmol? L-1 ammonium sulfate(gradient elution) as mobile phase.The flow rate was 1.0mL? min-1,the column temperature was 25℃ and the wavelength of UV detector was 254nm.RESULTS:The calibration curves for midazolam had a good linearity over the range of 0.05～ 25.00mg? L-1(r=0.999 8).The average recovery for midazolam was 99.29%.Both the intra-day RSD and inter-day RSD were less than 4%.The limits of quantitation for midazolam was 0.01mg? L-1.CONCLUS-ION:The method is sensitive,accurate,simple and rapid,and applicable for clinical blood drug level monitoring of midazolam.
ABSTRACT
OBJECTIVE:To evaluate the importance of monitoring serum concentration of Sodium Valproate(VPA)in the treatment of epileptic children.METHODS:Serum VPA concentration was determined by Chemiluminescence Enzyme Immunoassay(CLEIA),and the correlation between serum drug concentration and the daily dosage(mg?kg-1?d-1),dosage form,individual differences,blood sampling time and the clinical efficacy was analyzed.RESULTS:The serum VPA concentration which was lower than 50 mg?L-1 was found in 35.45% of the cases,and that which was higher than 100 mg?L-1 was found in 8.99%.CONCLUSION:The serum VPA concentration which was not in the therapeutic window can be resulted from multiple factors,and the monitoring results of the serum concentration should be analyzed comprehensively and made good use of.