ABSTRACT
Objective: To study the effect of different levels of Cu2+, Fe3+, Cr3+, and Ca2+ on mycelia biomass and the hypocrellin yield by submerged culture of Shiraia bambusicola. Methods: The fungis were cultured under 27°C and 130 r/min for 96 h with each level of metal ions. Results: The biomass and the hypocrellin yield were the highest with Cr3+ of 0.005%; Fe3+ of 0.003% and 0.005% contents were favorable to the biomass and the hypocrellin yield, respectively; Ca 2+ content of 0.03% was suitable for the mycelia ' growth and the hypocrellin yield benefited from the concentration of 0.005%; Cu2+ content of 0.03% was added, both the biomass and the hypocrellin yield reached the best. Conclusion: The highest mycelia dry weight and the hypocrellin yield could reach 15.28 g/L and 8.12 mg/g, respectively in addition of metal ion.
ABSTRACT
Objective To obtain anamorphosis of Shiraia bambusicola producing the component,hypocrellin A effectively.Methods A strain was isolated from the fruit of S.bambusicola by separating its hypha and was cultured by solid-state fermentation.One of the pure chemical compounds isolated from the stroma of wild S.bambusicola was studied on the basis of UV/VIS,IR,MS spectrometric analysis and physicochemical constants.Results The pigment is proved to be hypocrellin A.The isolate was determined as the stable anamorphosis strain 0258 of S.bambusicola.Conclusion A namorphosis strain of S.bambusicola is obtained and precisely appraised for the first time.This study will provide basis for the future massively industrialized hypocrellin production.
ABSTRACT
Objective To study the chemical constituents of the medicinal fungus Shiraia bambusicola.Methods Chromatography on silica column and preparative HPLC were used to purify the compounds;Spectroscopic methods including 1H-NMR,13CNMR,and MS were used to elucidate their structures.Results Nine compounds were isolated and identified as 3,6,8-trihydroxy-l-methylxanthone(1),3,8-dihydroxy-6-methoxy-1-methylxanthone(2),2,3,6,8-tetrahydroxy-l-methylxanthone(3),3,4,6,8-tetrahydroxy-l-methylxanthone(4),pregn5(10)-en-3?,17?,20?-triol(5),macrosphelide A(6),(+)-griseofulvin(7),griseophenone A(8),and 11,11′-dideoxyverticillin A(9).Conclusion Compounds 1-9 are obtained from the fungus S.bambusicola for the first time.