ABSTRACT
Objective: The effect of ethanol to material ratio on ethanol precipitation and sediment morphology of Shuanghuanglian preparation (SHLP) were researched, and the feasibility of comprehensive evaluation of ethanol precipitation sediment fractal dimension was discussed. Methods: For the SHLP ethanol precipitation process, HPLC and gravimetric analysis were used to investigate the effects of ethanol to material ratio on the overall retention of the index components and the liquid content of the filter cake. The direct observation, atomic force microscope observation and "area-perimeter" calculation method were used to investigate the influence of ethanol to material ratio on the apparent morphology, micro-morphology and fractal dimension of the precipitate. At the same time, Pearson correlation analysis method was used to investigate the correlation between the fractal dimension and the above evaluation index of ethanol precipitation effect. Results: With the increase of the ethanol to material ratio, the comprehensive retention of the index components and the fractal dimension of the ethanol precipitation precipitates was gradually increased, while the liquid content of the filter cake was gradually decreased. Therefore, the fractal dimension of sediment was positively correlated with the comprehensive retention rate of index components, but negatively correlated with the liquid content of the filter cake. Conclusion: The fractal dimension of ethanol precipitation sediment can be used as comprehensive evaluation index for reflecting the ethanol precipitation effect in the SHLP ethanol precipitation process, which can provide a reference for the visualized fine control research of the subsequent SHLP ethanol precipitation process.
ABSTRACT
We established a quality evaluation method for Shuanghuanglian preparations based on an effect-constituent index (ECI), which is guided by the clinical efficacy of Shuanghuanglian and a dose-efficacy correlation. An HPLC method was used to establish the quantitative fingerprint of Shuanghuanglian from different manufacturers and to determine the content of 10 fingerprint components, including baicalin, chlorogenic acid, forsythin, galuteolin, wogonin, forsythoside A, luteolin, caffeic acid, baicalein, and scutellarin. Using Staphylococcus aureus as biological model, the potency of Shuanghuanglian preparations was determined by antibiotic microbial assay. Using the method of PLC-DA, the efficacious antibacterial components were screened by "dose-efficacy" correlation analysis. According to the antibacterial potency and content of the antibacterial ingredients, combined with the method of the custom weight coefficient, ECI was calculated and verified. The results show that the antibacterial ECI can facilitate evaluation of the efficacy of Shuanghuanglian based on the composition of its contents, providing a new method for the quality control of traditional Chinese medicine.
ABSTRACT
OBJECTIVE: To study the feasibility of quantitative analysis of multi-components by single-marker (QAMS) in determination of flavones in Shuanghuanglian preparations by comparing the results assayed by QAMS and external standard method (ESM). METHODS: The analysis was performed on a Xtimate™ C18 column (4.6 mm × 250 mm, 5 μm)with methanol and water containing 0.2% phosphoric acid as the mobile phase in gradient elution. The flow rate was set at 1.0 mL · min-1. The UV detector wavelength was 274 nm, and the column temperature was 30°C. RESULTS: Within the linear ranges, the analysis results showed no significant difference between QAMS and ESM. CONCLUSION: The QAMS method is credible and will be a new quality evaluation pattern for the determination of multiple flavones in Shuanghuanglian preparations.
ABSTRACT
OBJECTIVE: To establish the item of phillyrin content determination in the quality standard of shuanghuanglian preparations. METHODS:The contents of phillyrin were determined by HPLC with ODS column and acetonitrile- water(25 : 75) as mobile phase.Detecting wavelength was 277nm. RESULTS: The calibration curve was linear in this experiment. The average recovery was 99. 1% with RSD of 0. 82% .CONCLUSION: This method is good in precision and satisfactory in isolation and repeatability and the result is accurate. It may be adopted in quality control of shuanghuanglian preparations.