Study on preparation of silver nanoparticles by biomass reduction of traditional Chinese medicinal materials and their antibacterial activity / 中草药

Objective: To use the extracts from traditional Chinese medicinal materials (TCMM) for reduction of Ag+ to obtain the corresponding silver nanomaterials and study its antibacterial activity. Methods: Nano-silver solution was prepared by Rhei Radix et Rhizoma and other 15 kinds of TCMM extracts, the size and distribution of silver nanoparticles were studied using HPPS, TEM, and UV. The antibacterial activities of silver nanoparticles were investigated by disk agar diffusion method and double dilution method. Results: Preparation rate and reaction rate of silver nanoparticles prepared with the water extract of Rhei Radix et Rhizoma, Polygoni Multiflori Radix Praeparata, Moslae Herba were ideal. Prepared silver nanoparticles with diameter about 100 nm, good uniformity, and good dispersibility have the obvious inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli. Among them, Polygoni Multiflori Radix Praeparata-silver nano has the strongest bacteriostatic effect. Conclusion: TCMM as a natural bio-reducing agents, can be used to prepare the silver nanoparticles.

Synthesis and characterization of silver nano particles from wrightia tinctoria.

In our present study the conventional (physical and chemical) method of nano particles synthesis was replaced with biological synthesis of silver nanoparticles by using the leaf extract of Wrightia tinctoria. New drug delivery system has been enhanced with noval techniques like nano particle synthesis and its role in drug delivery system, by exploiting the nanotechnology in particle synthesis. The aqueous extract of Wrightia tinctoria leaves was prepared and mixed with 1mM AgNO3 solution. After 48 hours the reduction of silver nitrate to silver nanoparticles (AgNPs) was confirmed by UV-visible spectrophotometer. The size of Silver nanoparticles were characterized by XRD and FTIR and the size were of 19 – 68nm. The biosynthesis of AgNPs using Wrightia tinctoria leaf extract is very simple and economic. This green chemistry approach is amenable to large scale commercial production. The use of environmentally benign and renewable plant material offers enormous benefits of eco-friendliness.