ABSTRACT
Objective To prepare a mesoporous silica-coated polypyrrole nanoparticles loaded with honokiol (PPy@MSN-HK) and evaluate their in vitro release behavior. Methods In this study, PPy@MSN-HK was obtained in three steps: First, prepared polypyrrole nanoparticles; Second, coated mesoporous silica shell on its surface; Third, absorbed honokiol. The TEM, particle size, zeta potential, drug loading, infrared spectroscopy, in vitro photothermal properties, and in vitro release characteristics were chosen as indexes to investigate its potential as antitumor nanocarries. The release profiles were analyzed by simulating factor (f2), and the dissolution profiles were fitted by a variety of commonly used mathematical models. Results The results showed that the prepared nanoparticles had uniform particle size and uniform size distribution. The average particle size was (220.4 ± 4.2) nm, polydispersity coefficient was 0.042 ± 0.010, zeta potential was (-21.1 ± 0.8) mV, drug loading was (2.58 ± 0.53)%, and entrapment efficiency was (75.04 ± 0.95)%, respectively. The results of in vitro photothermal experiments showed that with the constant laser power density, the temperature change value of nanoparticles suspension increased with the increase of nanoparticles concentration. This showed that PPy@MSN have a good photothermal effect. In vitro release test revealed that the two release curves were not similar, and fitting best with Ritger-Peppas eqution and Logistic eqution respectively. Conclusion The water solution method could be used to prepare PPy@MSN, which may provide a promising drug delivery strategy for tumor treatment.
ABSTRACT
Purpose:To study the feasibility of GM CSF and the tumor antigen activated dendritic cells in treating bladder tumor in rats.Methods:The DCs isolated from the spleen of T739 mice which were stimulated in vitro by syngenic granulocyte macrophage colony stimulating factor (GM CSF) and irradiated inactivated BTT739 Bladder tumor cells(Group D),were transfused intravenously once a week when BTT739 tumor cells were inoculated in mouse. Group A was established as control,group B and C were injected with GM CSF activated DCs and inactivated tumor vaccine.Results:In group D, the CTL activity against the BTT739 tumor and cytokine IL 4 and IFN ? was significantly higher than those in other groups ( P