ABSTRACT
Objective:To establish ion chromatography(IC) method for determination of the content of sodium glycerophosphate and phosphate salts in concentrated divitamins and sodium phosphate syrup. Methods:An AG19 -HC (50 mm× 4 mm) guard col-umn and an IonPac AS18 -HC (250 mm × 4 mm) ion analysis column were used, potassium hydroxide solution was applied as the mobile phase with gradient elution,and a conductance detector was used with suppression conductometric detection. Results:Sodium glycerophosphate and phosphate could be separated well under the chromatographic conditions. The linear range was 1.054-84.35 μg ·ml-1(r=0.999 4)for sodium glycerophosphate and 0.316 8-25.35 μg·ml-1(r=0.999 8)for phosphate. The average recovery was 99.35% (RSD=1.16%,n=9) and 98.96%(RSD=2.61%,n=9), respectively.Conclusion: The method is simple, rapid and accurate,and can be used for the quality control of concentrated divitamins and sodium phosphate syrup.
ABSTRACT
OBJECTIVE:To prepare temperature sensitive hydrogel which is of strength.METHODS:The hygrogel was prepared using CS,PVA and GP as matrix with concentration of CS(A),weight ratio of CS to PVA(B) and pH value(C) as factors and with initial gelatination temperature,strength and dehydration as indexes.The formula was optimized by orthogonal experiment and verified.RESULTS:The optimal formula was as follows:A 20 mg?mL-1,B 1 :1,C 7.2.The prepared hydrogel was fluid at 4 ℃ or room temperature while gelatinized at 37 ℃ within 10 min with strength of about 1.4 kPa.Gelatination time reduced along with the increase of temperature.The pH value had hardly changed during degradation in vitro within 28 d.CONCLUSION:The CS/PVA/GP hydrogel is simple and practical in preparation technique and is temperature sensitive and of strength.