Determination of quercetin and kaempferol in Stamen Neulumbinis by HPLC / 中成药

Objective: To determine quercetin and kaempferol in stamen neulumbinis by HPLC. Methods : Shim-pack CLC-ODS column was used. Mobile phase consisted of methanol-0.025% phosphate. UV detector wavelength was set at 370nm. Results : The chromatographic peaks of quercetin and kaempferol and other components were completely separated. The average recovery was 96.8% and RSD was 1.97%. Conclusion : The experiment provided new markers and a reliable method for the quality control of Stamen Neulumbinis.