ABSTRACT
Objective To construct the recombinant protein AtlM ( rAtlM) of Staphylococcal au-reus through prokaryotic expression system and to investigate its antibacterial activity in vitro.Methods The specific primers were designed according to atlM gene sequence of Staphylococcal aureus recorded in Gen-Bank, and atlM gene was amplified by PCR from the Staphylococcal aureus strain (ATCC25923).The re-combinant plasmid pET-32а(+)/atlM was constructed and transformed into Transetta ( DE3 ) to express AtlM after induced by IPTG .The expressed protein AtlM was further analyzed by SDS-PAGE and purified by electroeluting of bag filter.The minimal inhibitory concentrations (MICs) of rAtlM to ATCC25923 and oxac-illin-resistant S.aureus strain were determined by the broth microdilution method .S.aureus ATCC25923 strain and oxacillin-resistant S.aureus strain (final concentration of 5×105 CFU/ml) were exposed to rAtlM (50 μg/ml) respectively to test its antibacterial activity in vitro.Results The recombinant protein AtlM was expressed and purified successfully with a relative molecular weight of 80 ×103 and a concentration of 1.25 mg/L.The MICs of rAtlM to ATCC25923 strain and oxacillin-resistant S.aureus strain were 8 μg/ml and 64 μg/ml, respectively.In vitro test showed that rAtlM had inhibitory effects on the growth of ATCC25923 strain and oxacillin-resistant S.aureus strain after 1 h of intervention (P=0.004 and P=0.026, respectively), which lasted to 5 h for ATCC25923 strain (P=0.012) and 3 h for oxacillin-resistant strain (P=0.001).Conclusion This study shows that rAtlM has a certain antibacterial effects on S.aureus ATCC25923 strain and oxacillin-resistant S.aureus strain, suggesting a possibility of serving as antimicrobial agent.
ABSTRACT
Introducción :las infecciones osteoarticulares (IO) presentan elevada morbilidad. Staphylococcus aureus (SA) es la etiología más frecuente. La emergencia de cepas meticilino resistente de origen comunitario (SAMR-AC) representa un problema. En 2008 se comunica la primera serie nacional sobre etiología de las IO en niños. Objetivo: describir formas de presentación, tratamiento y evolución de IO por SA en niños hospitalizados en el Hospital Pediátrico del Centro Hospitalario Pereira Rossell (HP-CHPR) entre 2009 y 2012. Material y métodos: se identificaron muestras bacteriológicas con sospecha de IO en el Laboratoriode Bacteriología del HP-CHPR. Se incluyeron punciones óseas y articulares. Se analizaron características epidemiológicas, exámenes complementarios, tratamientos y evolución. Resultados: 283 muestras bacteriológicas. En 92 (32,5%) se identificó germen, 53 (63%) SA. Se analizaron 45: 38 S. aureus meticilino sensible (SAMS)y 7 SAMR-AC. Edad media 7,7 años. 34 (75,5 %) en miembros inferiores. 12 (27%) leucocitosis >15. 000 elementos/mm3, 30(67%) proteína C reactiva(PCR)>20mg/dL. Tratamiento empírico inicial 27 niños clindamicina + gentamicina. Las infecciones por SAMR-AC fueron más graves: focos múltiples (n=1),trombosis venosa profunda (n=2), shock sépticos (n=2), drenaje quirúrgico (3,7/niño). Estadía hospitalaria mayor (media 19,5 versus 15,2 días),requirieron más cuidado intensivo (5,2 versus 0,13 días) y uno falleció. Discusión: el porcentaje de aislamientos fue similar al comunicado en la literatura. A pesar de que SA sigue siendo el más frecuente, en nuestra serie SAMR-AC ha disminuido pero continua siendo virulento. Se debe tener alta sospecha para diagnóstico temprano y tratamiento oportuno.
Introduction: osteoarticular infections (OAIs) have ahigh morbidity. Staphylococcus aureus (SA) is themost frequent etiology. The emergence ofcommunity-associated methicillin-resistant (CA-MRSA)strains represents a problem. In 2008, the first nationalseries on the etiology of OAIs in children iscommunicated.Objective: describe the clinical presentations,treatment and evolution of the OAIs for SA in childrenhospitalized in the Hospital Pediátrico [PediatricHospital] of the Centro Hospitalario Pereira Rossell[Pereira Rossell Hospital Center] (HP-CHPR) between2009 and 2012.Materials and methods: bacteriological samples withsuspect OAI were identified at the bacteriologyLaboratory of the HP-CHPR. Lumbar and intra-articularpunctures were included. Epidemiologicalcharacteristics, complementary tests, treatments andevolution were analyzed.Results: 283 bacteriological samples. In 92 (32,5%)the germ was identified, 53 (63%) SA. 45 wereanalyzed: 38 methicillin-sensitive S. aureus (MSSA),and 7 CA-MRSA. Average age: 7.7 years. 34 (75.5%) inlower limbs. 12 (27%) leukocytosis>15.000 cell/mm3,30 (67%) CRP >20 mg/dL. Initial empiric therapy 27children clindamycin + gentamicin. The CA-MRSAinfections were more serious: multiple foci (n=1),deep venous thrombosis (n=2), septic shocks (n=2),surgical drainage (3,7/child)...
Subject(s)
Humans , Adolescent , Infant , Child, Preschool , Child , Drug Resistance, Microbial , Staphylococcal Infections/diagnosis , Staphylococcal Infections/physiopathology , Staphylococcal Infections/therapy , Osteoarthritis/diagnosis , Osteoarthritis/etiology , Osteoarthritis/therapy , Staphylococcus aureus , Child, HospitalizedABSTRACT
Bacterial esophagitis is an uncommon disease and has not been well characterized. Bacterial infection of the esophagus is usually presented as a superimposed infection upon a preexisting viral or fungal esophagitis and most patients are immunocompromised hosts. A 67-year-old man was admitted for retrosternal pain and hematemesis, who had a past history of long-standing diabetes mellitus and end stage renal disease, also had a history of steroid medication. Extensive esophageal ulcerations of the mucosa were visualized by endoscopy. Staphylococcus aureus grew in blood culture. After the 2 weeks of antibiotics treatment, he was successfully recovered without any sequelae. Due to its rarity, this case is herein reported with a review of the corresponding literature.